Abstract:
:To determine the effect of organics (yeast extract) on microbial community during chalcopyrite bioleaching at different temperature, real-time polymerase chain reaction (PCR) was employed to analyze community dynamics of major bacteria applied in bioleaching. The results showed that yeast extract exerted great impact on microbial community, and therefore influencing bioleaching rate. To be specific, yeast extract was adverse to this bioleaching process at 30°C due to decreased proportion of important chemolithotrophs such as Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. However, yeast extract could promote bioleaching rate at 40°C on account of the increased number and enhanced work of Ferroplasma thermophilum, a kind of facultative bacteria. Similarly, bioleaching rate was enhanced under the effect of yeast extract at 50°C owing to the work of Acidianus brierleyi. At 60°C, bioleaching rate was close to 100% and temperature was the dominant factor determining bioleaching rate. Interestingly, the existence of yeast extract greatly enhanced the relative competitiveness of Ferroplasma thermophilum in this complex bioleaching microbial community.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Li Q,Tian Y,Fu X,Yin H,Zhou Z,Liang Y,Qiu G,Liu J,Liu H,Liang Y,Shen L,Cong J,Liu Xdoi
10.1007/s00284-011-9960-ysubject
Has Abstractpub_date
2011-08-01 00:00:00pages
164-72issue
2eissn
0343-8651issn
1432-0991journal_volume
63pub_type
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