Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp. israelensis in Asticcacaulis excentricus.

Abstract:

:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry11Aa gene. The genes were introduced as an operon, but although mRNA was detected for both genes, no Cyt1Aa toxin was detected. Both proteins were expressed using a construct in which a promoter was inserted upstream of each gene. Recombinant A. excentricus expressing both toxins was found to be approximately twice as toxic to third instar larvae of Culex quinquefasciatus as transformants expressing just Cry11Aa.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Zheng D,Valdez-Cruz NA,Armengol G,Sevrez C,Munoz-Olaya JM,Yuan Z,Orduz S,Crickmore N

doi

10.1007/s00284-006-0352-7

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

58-62

issue

1

eissn

0343-8651

issn

1432-0991

journal_volume

54

pub_type

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