Abstract:
:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry11Aa gene. The genes were introduced as an operon, but although mRNA was detected for both genes, no Cyt1Aa toxin was detected. Both proteins were expressed using a construct in which a promoter was inserted upstream of each gene. Recombinant A. excentricus expressing both toxins was found to be approximately twice as toxic to third instar larvae of Culex quinquefasciatus as transformants expressing just Cry11Aa.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Zheng D,Valdez-Cruz NA,Armengol G,Sevrez C,Munoz-Olaya JM,Yuan Z,Orduz S,Crickmore Ndoi
10.1007/s00284-006-0352-7subject
Has Abstractpub_date
2007-01-01 00:00:00pages
58-62issue
1eissn
0343-8651issn
1432-0991journal_volume
54pub_type
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