Abstract：
:Fragments of Synechocystis-DNA driving salt-induced gene expression in E. coli were isolated with translational fusions to a 'lacZ gene. One fragment (fragment 19) showed a NaCl-dependent activation of betaGal expression with the maximum of a ninefold increase in enzyme activity. A similar induction was triggered by the nonionic osmolyte sucrose, indicating an osmotically dependent activation. On the contrary, transcriptional activity of the DNA fragment 19 was only slightly enhanced under salt stress conditions, suggesting a posttranscriptional mechanism of induction. Primer extension assay was performed to identify the transcription initiation site. Upstream regions share weak homology to the "-10" hexamer consensus of E. coli sigma70 promoters. The most thermodynamically stable secondary structure for the nontranslated part of the mRNA indicated that potential translation initiation sites might be blocked, leading to a low basal translation, whereas osmotic stress-induced changes of mRNA structure could be involved to increase translation. In order to analyze the function of fragment 19 in Synechocystis, promoter-probe plasmids were constructed allowing the stable integration of transcriptional and translational reporter gene fusions into the cyanobacterial chromosome. Quantitative assessment of reporter gene expression revealed a weak constitutive promoter activity of fragment 19 in Synechocystis. Sequence analysis showed that fragment 19 comprises 223 bp of the ORF sll0747 of the Synechocystis genome.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Milkowski C,Quinones A,Hagemann Mdoi
10.1007/s002849900347subject
Has Abstractpub_date
1998-08-01 00:00:00pages
108-16issue
2eissn
0343-8651issn
1432-0991journal_volume
37pub_type
杂志文章abstract：:An endolithic bacterium, strain RSBr-1, was isolated from the inside of a piece of red sandstone from coastal areas of Scotland. RSBr-1 was gram negative, oxidase and catalase positive, and cells were non-motile rods. Sodium was required for growth. The optimum sodium chloride concentration and pH for growth were 4% a...
journal_title：Current microbiology
pub_type： 杂志文章
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journal_title：Current microbiology
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abstract：:Panax notoginseng has long been used as a Chinese herb with high medicinal value. The endophytic bacteria in this medicinal plant have multiple biological functions. High-throughput sequencing is a rapidly evolving technique that helps profile the endophytic bacterial community structure of medicinal plants. However, ...
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journal_title：Current microbiology
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doi：10.1007/s00284-005-4531-8
更新日期：2005-07-01 00:00:00
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journal_title：Current microbiology
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF02602456
更新日期：1980-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9347-x
更新日期：2009-04-01 00:00:00
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更新日期：2008-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：1994-11-01 00:00:00
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journal_title：Current microbiology
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更新日期：2015-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Current microbiology
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更新日期：2007-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900193
更新日期：1997-06-01 00:00:00
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journal_title：Current microbiology
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更新日期：2003-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02033-z
更新日期：2020-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010180
更新日期：2001-01-01 00:00:00
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更新日期：2003-03-01 00:00:00
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journal_title：Current microbiology
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更新日期：2001-06-01 00:00:00
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更新日期：2001-09-01 00:00:00
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更新日期：2019-12-01 00:00:00
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更新日期：2015-11-01 00:00:00
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journal_title：Current microbiology
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更新日期：2020-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900319
更新日期：1998-06-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2011-12-01 00:00:00
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journal_title：Current microbiology
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更新日期：2001-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：1997-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900384
更新日期：1998-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0707-4
更新日期：2015-02-01 00:00:00
