Abstract:
:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a protein of 366 amino acids (40,687 Da) with a pI of 9.17. The EstA signal peptide was 31 amino acids long, and the mature esterase sequence is 335 amino acids long (37.5 kDa). The conserved catalytic serine residue of EstA is in position 210. The EstA sequence was similar to that of the carboxylesterase from Acinetobacter calcoaceticus (75% identity, 85% similarity), Archaeoglobus fulgidus (37% identity, 59% similarity), and Mycobacterium tuberculosis (35% identity, 51% similarity). These enzymes contained the conserved motif G-X(1)-S-X(2)-G carrying the active-site serine of hydrolytic enzyme. The EstA activity in A. lwoffii I6C-1 remains constant throughout the stationary phase, and the activity in E. coil BL21 (DE3) with pEM1 was similar to A. lwoffii I6C-1.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Kim HE,Lee IS,Kim JH,Hahn KW,Park UJ,Han HS,Park KRdoi
10.1007/s00284-002-3886-3keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
291-5issue
4eissn
0343-8651issn
1432-0991journal_volume
46pub_type
杂志文章abstract::Bacteria were isolated from rabbit faeces using equine caecal fluid as a growth medium. Two new isolates of the genus Streptococcus are described in terms of their biochemical properties. One of these has a 16S rRNA gene with 97.7%, and the other 98.5%, identity to Streptococcus thoraltensis. While S. thoraltensis has...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9619-0
更新日期:2010-10-01 00:00:00
abstract::Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1225-y
更新日期:2017-05-01 00:00:00
abstract::Mycobacteria are widely present in diverse aquatic habitats, where they can survive for months or years while some species can even proliferate. The resistance of different mycobacterial species to disinfection methods like chlorination or ozonation could result in their presence in the final tap water of consumers. I...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0427-1
更新日期:2013-12-01 00:00:00
abstract::We report on a novel bacterium, isolated during a screen for environmental isolates of Bacillus thuringiensis, that possesses a novel filamentous structure. Nucleotide sequence from the isolate's 16S rRNA gene places the bacterium unambiguously within the Bacillus thuringiensis/Bacillus cereus group. Phase-contrast an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4103-8
更新日期:2003-10-01 00:00:00
abstract::Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9158-0
更新日期:2008-08-01 00:00:00
abstract::The influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from Lactobacillus fermentum CRL 251 was studied. Stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; sc...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900118
更新日期:1996-11-01 00:00:00
abstract::Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900082
更新日期:1996-08-01 00:00:00
abstract::A 4.5-kilobase DNA fragment from Buchnera aphidicola, the endosymbiont of the aphid Schizaphis graminum, was cloned and sequenced. On the basis of homology to Escherichia coli, the following genes were found in the order listed: aroH-thrS-infC-rpmI-rplT. AroH corresponds to the E. coli tryptophan-inhibited 3-deoxy-D-a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00295507
更新日期:1995-05-01 00:00:00
abstract::A screen of thermophilic lactic acid bacteria identified Streptococcus thermophilus strain ST110 as the putative producer of a bacteriocin with high level of activity against pediococci. Thermophilin 110 was isolated from culture supernatant after 16 h of growth and partially purified by a chloroform extraction proced...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4540-7
更新日期:2005-09-01 00:00:00
abstract::An in vitro study was conducted to examine the metabolism of histidine (His) by mixed rumen bacteria (B), mixed rumen protozoa (P), and a combination of the two (BP). Rumen microorganisms were collected from fistulated goats fed with lucerne cubes (Medicago sativa) and a concentrate mixture twice a day. Microbial susp...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010170
更新日期:2001-01-01 00:00:00
abstract::Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsie...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9262-1
更新日期:2009-01-01 00:00:00
abstract::An aerobic, gram-reaction-negative, rod-shaped bacterium, designated strain Z6(T), was isolated from sediment collected at Mohe Basin, China. And its taxonomic position was investigated by applying a polyphasic approach. Growth occurs at 10-45 °C (optimum, 30 °C), at pH 6.0-11.0 (optimum, pH 7.0) and in the presence o...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1074-0
更新日期:2016-09-01 00:00:00
abstract::The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9643-0
更新日期:2010-12-01 00:00:00
abstract::Nine phage isolates infectious for Vibrio vulnificus and falling into four morphological groups were isolated from estuarine waters collected in Louisiana. Of the 60 V. vulnificus strains tested, 87% were susceptible to one or more of the isolates. With the exception of V. fluvialis, Vibrio species other than vulnific...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00369859
更新日期:1995-06-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis (SE) infection in humans is often associated with the consumption of contaminated poultry products. Binding of the bacterium to the intestinal mucosa is a major pathogenic mechanism of Salmonella in poultry. Transposon mutagenesis identified flgC as a potential binding mutant of...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0521-z
更新日期:2014-05-01 00:00:00
abstract::Saccharopolyspora erythraea, a mycelium-forming actinomycete, produces a clinically important antibiotic erythromycin. Extensive investigations have provided insights into erythromycin biosynthesis in S. erythraea, but knowledge of its morphogenesis remains limited. By gene inactivation and complementation strategies,...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0410-x
更新日期:2013-12-01 00:00:00
abstract::Several di-, tri-, and oligopeptides were incubated individually in vitro with rumen fluid from two sheep receiving a mixed grass hay/concentrate diet and with washed cells of Prevotella (formerly Bacteroides) ruminicola M384 and P. ruminicola B(1)4. The rates of breakdown of most peptides were similar in the rumen fl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01576265
更新日期:1993-06-01 00:00:00
abstract::Campylobacter coli cells are characterized by a comma, or spiral shape, and a single polar flagellum. Here we report stable spontaneous changes in morphology to an aflagellated straight rod. The ability to colonize the chick cecum was lost. The atypical cells were compared with the original C. coli 67 culture from whi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4527-4
更新日期:2005-09-01 00:00:00
abstract::To investigate the correlation between mutations in promoter, attenuator, and the AmpC enzyme overproduction in Escherichia coli. ampC Promoters from 4 Escherichia coli clinical isolates were cloned upstream to the chloramphenicol acetyltransferase (CAT) gene in pCAT3 reporter plasmid. Promoter strengths were measured...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9426-7
更新日期:2009-09-01 00:00:00
abstract::The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cu...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900258
更新日期:1997-11-01 00:00:00
abstract::With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900272
更新日期:1998-01-01 00:00:00
abstract::This report presents results from a study on the bacterial diversity of cigarette brands collected from military personnel during the U.S. Army's investigation of a series of cases of acute eosinophilic pneumonitis in military personnel deployed in Operation Iraqi Freedom. Eight species of Bacillus, including five new...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4491-z
更新日期:2005-07-01 00:00:00
abstract::Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900319
更新日期:1998-06-01 00:00:00
abstract::Multi-drug resistance and transfer of mobile genetic elements among enteric pathogens is being reported to have increased rapidly. Commensal Escherichia coli was previously known to acquire mobile genetic elements from other genus/species. E. coli is also capable of disseminating these elements containing antimicrobia...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01676-x
更新日期:2019-06-01 00:00:00
abstract::A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogeneti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9469-9
更新日期:2009-11-01 00:00:00
abstract::The diversities of soil bacterial communities in the Phragmites australis, Phalaris arundinacea, and non-plantation constructed wetlands were compared and analyzed by through high-throughput Illumina sequencing. At the phylum level, the ten dominant bacterial phyla in the three types of constructed wetlands were the s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01634-7
更新日期:2019-03-01 00:00:00
abstract::The goal of this study was to evaluate how two new hydrolysates from poultry by-products act on ten lactobacilli growth kinetics when supplemented to the growth medium. These effects were compared with ones induced by two most common commercial hydrolysates, i.e., tryptone and peptone. Growth medium, supplemented with...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0445-z
更新日期:2014-01-01 00:00:00
abstract::Bacillus sp. TYF-LIM-B05, which is isolated from spoilage vinegar, is resistant to high temperature, high concentrated alcohol, acid, and salt, and can produce ethanol from mono-, di-, polysaccharide, and complex biomass as the sole carbon source. Thus, this strain is a potential candidate for consolidated bioprocessi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01833-2
更新日期:2020-03-01 00:00:00
abstract::Together with the worldwide Washoku (traditional Japanese foods and drinks) boom, interest in sake, a traditional Japanese alcoholic drink, is increasing around the world. There are few scientific analyses and studies on the production of sake or the final product itself. We show the diversity of bacterial contaminant...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-019-01718-4
更新日期:2020-04-01 00:00:00
abstract::Vegetative insecticidal protein (Vip3) from Bacillus thuringiensis shows high activity against lepidopteran insects. Cytolytic δ-endotoxin (Cyt) also has high toxicity to dipteran larvae and synergism with other crystal proteins (Cry), but synergism between Cyt and Vip3 proteins has not been tested. We analyzed for sy...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0070-7
更新日期:2012-04-01 00:00:00