The Effect of the Simultaneous Addition of Molybdenum and Tungsten to the Culture Medium on the Formate Dehydrogenase Activity from Methylobacterium sp. RXM

Abstract:

:Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM and for molybdenum concentration between 0.6 and 0.9 µM. The negative polynomial parameter (beta2) for tungsten compared with the positive polynomial parameter (beta1) for molybdenum on the FDH activity suggested that the latter metal exerts a stronger influence on the enzyme stimulation than the tungsten metal. A negative interaction between both metals was found, suggesting that tungsten and molybdenum shared an antagonistic effect on the enzyme activity.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Gírio FM,Roseiro JC,Silva AI

doi

10.1007/s002849900319

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

337-40

issue

6

eissn

0343-8651

issn

1432-0991

pii

CM2733

journal_volume

36

pub_type

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