Abstract：
:The mutation of Agrobacterium radiobacter cells exposed to chrysotile asbestos was examined by the random amplified polymorphic DNA (RAPD) method. Approximately 1.4 kbp of DNA in A. radiobacter, which was not amplified strongly in the cells that were not exposed to asbestos, was amplified in the cells that were exposed to asbestos. Mutation in genomic DNA of A. radiobacter was found to be induced by asbestos. Specific DNA that was amplified by asbestos present in PCR products and that which exists latently in genomic DNA were cloned, and these sequences were then determined and compared. It was shown that one of the mutations by the asbestos in the A. radiobacter occurred only in the primer annealed region and was a point mutation or deletion.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Yoshida N,Naka T,Sengoku T,Ogawa Kdoi
10.1007/pl00021058keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
398-402issue
6eissn
0343-8651issn
1432-0991journal_volume
42pub_type
杂志文章abstract：:Nine structurally similar macrolide antibiotics were tested at a concentration of 0.5 microg/ml for their relative inhibitory effects on ribosome functions in Staphylococcus aureus cells. Eight of the compounds examined inhibited protein synthesis at this concentration. Seven of the nine compounds were also effective ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900402
更新日期：1998-12-01 00:00:00
abstract：:The pathogenic capability of the tangerine pathotype of Alternaria alternata relies on the production of host-selective ACT toxin. Inoculation of A. alternata in leaves of the citrus quickly induced rapid lipid peroxidation, accumulation of hydrogen peroxide (H(2)O(2)), and cell death, indicative of host defensive res...
journal_title：Current microbiology
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doi：10.1007/s00284-010-9795-y
更新日期：2011-03-01 00:00:00
abstract：:Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein wa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0570-3
更新日期：2014-08-01 00:00:00
abstract：:A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01577445
更新日期：1994-11-01 00:00:00
abstract：:Variable-number-of-tandem-repeats (VNTR) markers are increasingly being used in population genetic studies of bacteria. They were recently developed for Pasteuria ramosa, an endobacterium that infects Daphnia species. In the present study, we genotyped P. ramosa in 18 infected hosts from the United Kingdom, Belgium, a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9104-1
更新日期：2008-05-01 00:00:00
abstract：:The archaeal diversity in salt pan sediment from Mumbai, India, was investigated by 16S rDNA-dependent molecular phylogeny. Small-subunit rRNA (16S rDNA) from salt pan sediment metagenome were amplified by polymerase chain reaction (PCR) using primers specific to the domain archaea. Thirty-two unique phylotypes were o...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9167-z
更新日期：2008-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840110218
更新日期：2001-04-01 00:00:00
abstract：:Although neutral mine drainage is the less frequent subject of the interest than acid mine drainage, it can have adverse environmental effects caused mainly by precipitation of dissolved Fe. The aim of the study was to characterize the composition of bacterial population in environment with high concentration of iron ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1472-6
更新日期：2018-08-01 00:00:00
abstract：:Because of heavy use of antimicrobials, antimicrobial resistance in bacteria has become of great concern. The effect of some widely used food additives such as sucralose on bacteria in the gut and the environment has also drawn increasing attention. In this study, we investigated the interaction between antimicrobials...
journal_title：Current microbiology
pub_type： 信件
doi：10.1007/s00284-017-1255-5
更新日期：2017-07-01 00:00:00
abstract：:A novel facultative anaerobic, Gram-stain-positive coccus, strain JS71T, was isolated from the human subgingival dental plaque of a periodontitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence had high sim...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01778-6
更新日期：2019-12-01 00:00:00
abstract：:This study investigated the antifungal effects of cement paste containing Paenibacillus polymyxa E681 against Aspergillus niger, a deleterious fungus commonly found in cement buildings and structures. To test the antifungal effects, cement paste containing P. polymyxa E681 was neutralized by CO2 gas, and the fungal gr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0604-x
更新日期：2014-10-01 00:00:00
abstract：:A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogeneti...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9469-9
更新日期：2009-11-01 00:00:00
abstract：:Lectins LI and LII, localized on the surface of the nitrogen-fixing soil bacterium Bacillus polymyxa 1460, were shown to possess proteolytic activity. A relationship was found between the proteolytic and hemagglutinating activities of the lectins. Blocking of hemagglutinating activity with specific carbohydrate hapten...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010128
更新日期：2000-10-01 00:00:00
abstract：:A Gram-negative, non-motile, rod-shaped, aerobic bacterial strain, designated S7-3-3T, was isolated from apple orchard soil in Gyeongsangnam-do province, South Korea, and was characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S7-3-3T belong...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1434-z
更新日期：2018-06-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900127
更新日期：1996-12-01 00:00:00
abstract：:An alkaline phosphatase (phoA) gene from Zymomonas mobilis was isolated in Escherichia coli CC118 by use of the plasmid Bluescript KS+. The origin of the 6.4-kb DNA fragment in pZAP1 from the chromosome of Z. mobilis was confirmed by Southern blotting and hybridization studies. The Z. mobilis phoA gene was localized a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01575864
更新日期：1992-11-01 00:00:00
abstract：:Several rhizobial strains possess the ability to modulate leguminous plants ethylene levels by producing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase. While the effect of ACC deaminase has been studied in several rhizobia belonging to the Alphaproteobacteria class, not much is understood about its impa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1474-4
更新日期：2018-08-01 00:00:00
abstract：:Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9158-0
更新日期：2008-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900462
更新日期：1999-11-01 00:00:00
abstract：:A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9T, was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9T belonge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1246-6
更新日期：2017-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0205-5
更新日期：2012-11-01 00:00:00
abstract：:In the present study, probiotic, safety and functional characteristics of eight indigenous bifidobacterial isolates were compared to identify suitable strains for functional food application. Among the isolates, six strains of Bifidobacterium longum and one each of Bifidobacterium breve and Bifidobacterium bifidum wer...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1615-9
更新日期：2019-03-01 00:00:00
abstract：:Strain NHI-24(T) was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24(T) is closely related to members of the genus Niabella: N. dril...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0565-0
更新日期：2014-08-01 00:00:00
abstract：:Rhizobium sp. SIN-1, a nitrogen-fixing symbiont of Sesbania aculeata and other tropical legumes, carries two copies of nodD, both on a sym plasmid. We have isolated these two nodD genes by screening a genomic library of Rhizobium sp. SIN-1 with a nodD probe from Sinorhizobium meliloti. Nucleotide sequence and the dedu...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3772-z
更新日期：2002-11-01 00:00:00
abstract：:Saccharopolyspora erythraea, a mycelium-forming actinomycete, produces a clinically important antibiotic erythromycin. Extensive investigations have provided insights into erythromycin biosynthesis in S. erythraea, but knowledge of its morphogenesis remains limited. By gene inactivation and complementation strategies,...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0410-x
更新日期：2013-12-01 00:00:00
abstract：:The regulation of the recA gene expression in the obligately anaerobic rumen bacterium Prevotella ruminicola was investigated by monitoring the recA-specific transcript level. P. ruminicola recA forms a monocistronic unit, but no SOS-box sequences resembling those of Escherichia coli or Bacillus subtilis can be identi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900306
更新日期：1998-05-01 00:00:00
abstract：:Leucoagaricus gongylophorus, the fungus cultured by the leaf-cutting ant Atta sexdens, produces polysaccharidases that degrade leaf components by generating nutrients believed to be essential for ant nutrition. We evaluated pectinase, amylase, xylanase, and cellulase production by L. gongylophorus in laboratory cultur...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0431-1
更新日期：2006-07-01 00:00:00
abstract：:In this study, fatty-acid content and patterns were analyzed in order to distinguish Nostoc flagelliforme, an edible terrestrial cyanobacterium, from other Nostoc species and representatives typical of its close neighbors (genera Anabaena, Microcystis, and Synechococcus). According to the Kenyon-Murata classification ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4059-8
更新日期：2003-12-01 00:00:00
abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) displays important functional diversity in mammalian and plants. So far, however, studies on GAPDH have not included the halotolerant, unicellular green alga Dunaliella salina. In the present study, a GAPDH cDNA was cloned and sequenced from D. salina. It was 1394 bp lo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9333-3
更新日期：2009-05-01 00:00:00
abstract：:Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006756
更新日期：1998-03-01 00:00:00
