Abstract:
:Nine structurally similar macrolide antibiotics were tested at a concentration of 0.5 microg/ml for their relative inhibitory effects on ribosome functions in Staphylococcus aureus cells. Eight of the compounds examined inhibited protein synthesis at this concentration. Seven of the nine compounds were also effective in blocking formation of the 50S ribosomal subunit. Roxithromycin and 14-hydroxy clarithromycin inhibited protein synthesis to a greater extent than they affected 50S subunit formation. Conversely, the compound 11, 12-carbonate-3 deoxy-clarithromycin affected 50S assembly more than translation. Only clarithromycin had any effect on 30S ribosomal subunit assembly. The decline in growth rate and cell number was proportional to the effect on ribosome formation or function by each compound. These inhibitory activities can be related to structural differences between these macrolide antibiotics.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Champney WS,Tober CL,Burdine Rdoi
10.1007/s002849900402subject
Has Abstractpub_date
1998-12-01 00:00:00pages
412-7issue
6eissn
0343-8651issn
1432-0991journal_volume
37pub_type
杂志文章abstract::Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleoti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1208-z
更新日期:2017-04-01 00:00:00
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journal_title:Current microbiology
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abstract::Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not pr...
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更新日期:2003-09-01 00:00:00
abstract::The dicarboxylic acids malate and fumarate increase ruminal pH, reduce methane production, increase propionate and total volatile fatty acid (VFA) production, and reduce lactic acid accumulation in a manner similar to ionophores. These acids stimulate the ruminal bacterium Selenomonas ruminantium to ferment lactate to...
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journal_title:Current microbiology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
abstract::We develop a method to capture marine bacterial strains at high efficiency to replace the conventional two-step collecting method. Lab-made, Fe3O4 magnetic beads were used to firstly verify the feasibility of capture in artificial seawater, using Bacillus velezensis. Almost 100% of the bacteria could be captured and s...
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journal_title:Current microbiology
pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
abstract::The Bacillus subtilis pur operon repressor (PurR) has a PRPP (5-phosphoribosyl 1-pyrophosphate) binding motif at residues 199-211. Two PurR PRPP binding region mutations (D203A and D204A) were constructed, and the effects on binding of repressor to the pur operon control site in vitro and on regulation of pur operon e...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010091
更新日期:2000-07-01 00:00:00
abstract::The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in th...
journal_title:Current microbiology
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
abstract::Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pl...
journal_title:Current microbiology
pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
abstract::Ruminal proteolysis and subsequent amino acid degradation represent considerable economic loss in ruminant production. The hyper ammonia-producing bacteria (HAB) are largely responsible for amino acid deamination in the rumen. HAB can be controlled with ionophores, but they are also susceptible to antimicrobial plant ...
journal_title:Current microbiology
pub_type: 杂志文章
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更新日期:2010-08-01 00:00:00
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更新日期:2011-08-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02013-3
更新日期:2020-09-01 00:00:00
abstract::Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus pr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3789-3
更新日期:2003-04-01 00:00:00
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更新日期:2003-10-01 00:00:00
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journal_title:Current microbiology
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更新日期:2020-05-01 00:00:00
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journal_title:Current microbiology
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更新日期:2006-02-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli (E. coli) O157:H7, a gram-negative bacteria identified as a foodborne pathogen causing severe disease is of great concern worldwide. The pathogenicity of E. coli O157:H7 is due to the presence of some virulence factors and its ability to form biofilm which resist antimicrobial compou...
journal_title:Current microbiology
pub_type: 杂志文章,评审
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更新日期:2017-12-01 00:00:00
abstract::Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900082
更新日期:1996-08-01 00:00:00
abstract::Exposure to ethanol is a stress condition that Salmonella typhimurium often encounters during its life cycle. Food, beverage, drugs, and cosmetics have a long history of using alcohols to control pathogens. Ethanol is also commonly used for disinfecting medical instruments. This study was conducted to evaluate the eth...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9314-6
更新日期:2009-03-01 00:00:00
abstract::A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterizatio...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900470
更新日期:1999-12-01 00:00:00
abstract::Fourteen forms of endopolygalacturonases were purified from the culture medium of Sclerotinia sclerotiorum with ion exchange chromatographies. Individual forms differ in their isoelectric point but exhibit similar molecular masses. On the basis of the cross-reactions to antibodies raised against a purified acidic endo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900107
更新日期:1996-10-01 00:00:00
abstract::A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02210-0
更新日期:2020-11-01 00:00:00
abstract::Lipids contribute significantly to the pathogenesis of fungal infectious diseases and an understanding of lipid metabolism occurring in fungal pathogens can help the development of more efficient antifungal therapeutic strategies. In this study, the effect of culture age on the distribution of fatty acids among differ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9052-1
更新日期:2008-02-01 00:00:00
abstract::Maize root colonization by two fluorescentPseudomonas strains M.3.1. and TR335, isolated respectively frommaize and tomato roots, were studied in hydroponic conditions. Each bacteriumwas inoculated separately, and three different colonization areas werestudied: nutrient solution, rhizoplane, and endorhizosphere. The t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900185
更新日期:1997-05-01 00:00:00