Abstract:
:The interactions between the proteolytic X2L strain of Oenococcus oeni and the non-proteolytic 12p strain of Pediococcus pentosaceus were assayed. The characteristics of cell growth, protein degradation, and amino acid production of both strains were determined in pure and mixed cultures. O. oeni showed poor cell growth and greater ability in the release of amino acids to the extracellular medium, whereas P. pentosaceus showed a higher yield in cell production with a decrease in the amino acid concentration in the medium. P. pentosaceus especially consumed essential amino acids for growth, and O. oeni released several of the essential amino acids important for growth of P. pentosaceus. In the mixed culture, mutualism was observed. The higher activity of the proteolytic system of O. oeni in mixed culture produced an increase in cell growth and in the amount of essential amino acids released. These findings provide new knowledge about the metabolic interactions between lactic acid bacteria isolated from wine when proteins are degraded in mixed bacterial populations.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Fernández PA,de Nadra MCdoi
10.1007/s00284-005-0079-xkeywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
86-91issue
2eissn
0343-8651issn
1432-0991journal_volume
52pub_type
杂志文章abstract::The aim of this study is to investigate the combination of cefepime and sulbactam. Sulbactam, when administered , will effectively inhibit all Extended-spectrum beta lactamases (ESBLs) of the microorganism, while cefepime will inhibit the growth of the resistant microorganisms since it will not be hydrolyzed by OXA-48...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02094-0
更新日期:2020-09-01 00:00:00
abstract::Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleoti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1208-z
更新日期:2017-04-01 00:00:00
abstract::Brevibacterium halotolerans is currently classified as a member of the Brevibacterium genus, a genus that groups together many bacterial species of similar morphology but diverse biochemical and physiological features. Here we suggest, based on multiple gene sequencing and microbial and biochemical characterization of...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1143-4
更新日期:2017-01-01 00:00:00
abstract::The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected str...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1428-2
更新日期:2018-05-01 00:00:00
abstract::Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and ex...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0114-5
更新日期:2002-09-01 00:00:00
abstract::The complex gut microbiota plays a key role in host metabolism and health. However, the core microbial communities in the different aged Bactrian camels remain totally unclear. We used high-throughput 16S rRNA gene sequencing to examine the temporal variability of the fecal microbiota in Bactrian camels. At 2 months o...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01689-6
更新日期:2019-07-01 00:00:00
abstract::A systematic evaluation of the ability of different bacterial genera to transform 2,4,6-trinitrotoluene (TNT), and grow in its presence, was conducted. Aerobic Gram-negative organisms degraded TNT and evidenced net consumption of reduced metabolites when cultured in molasses medium. Some Gram-negative isolates transfo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900216
更新日期:1997-08-01 00:00:00
abstract::Campylobacter jejuni is a major food-borne pathogen that causes human gastroenteritis in many developed countries. In our study, we applied multilocus sequence typing (MLST) technology to 167 C. jejuni isolates from diverse sources in Eastern China to examine their genetic diversity. MLST defined 94 sequence types (ST...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0853-3
更新日期:2015-09-01 00:00:00
abstract::The low molecular weight (LMW) heat shock protein (HSP), HSP16.6, in the unicellular cyanobacterium, Synechocystis sp. PCC 6803, protects cells from elevated temperatures. A 95% reduction in the survival of mutant cells with an inactivated hsp16.6 was observed after exposure for 1 h at 47 degrees C. Wild-type cell sur...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910056
更新日期:2000-04-01 00:00:00
abstract::The dimorphic yeast Yarrowia lipolytica has become an emerging cell factory for recombinant proteins production. Expression vectors involving LIP2 promoter (pLIP2) have been developed and used successfully. However, the relationship between dimorphic transition (i.e., cell morphology) and pLIP2 regulation is still unc...
journal_title:Current microbiology
pub_type: 信件
doi:10.1007/s00284-017-1207-0
更新日期:2017-03-01 00:00:00
abstract::A bacterium capable of utilizing p-cresol as sole source of carbon and energy was isolated from soil and identified as a Bacillus species. The organism also utilized phenol, o-cresol, m-cresol, 4-hydroxybenzoic acid, and gentisic acid as growth substrates. The organism degraded p-cresol to 4-hydroxybenzoic acid, which...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0309-x
更新日期:2006-12-01 00:00:00
abstract::Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacter...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0627-z
更新日期:2007-06-01 00:00:00
abstract::The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9831-y
更新日期:2011-04-01 00:00:00
abstract::The Bacillus subtilis pur operon repressor (PurR) has a PRPP (5-phosphoribosyl 1-pyrophosphate) binding motif at residues 199-211. Two PurR PRPP binding region mutations (D203A and D204A) were constructed, and the effects on binding of repressor to the pur operon control site in vitro and on regulation of pur operon e...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010091
更新日期:2000-07-01 00:00:00
abstract::The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9107-y
更新日期:2008-05-01 00:00:00
abstract::Betel vine is an edible creeper used in folk medicine to aid digestion since time immemorial. It is an ideal candidate deemed for the bioprospection of endophytic microorganisms with valuable attributes. This study aimed at the characterization of potential bacteria from fermented betel leaves. We report the presence ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02270-2
更新日期:2021-01-01 00:00:00
abstract::Expression from the secA gene, encoding a key component of the general secretory pathway of Escherichia coli, is influenced by the secretion status of the cell, autogenous translational repression, and translational coupling to the upstream gene, X. SecA binds to its mRNA in a region overlapping its ribosome binding s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900413
更新日期:1999-02-01 00:00:00
abstract::All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02150-9
更新日期:2020-11-01 00:00:00
abstract::Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observe...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00294281
更新日期:1995-08-01 00:00:00
abstract::The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cu...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900258
更新日期:1997-11-01 00:00:00
abstract::Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted levansucrase (LsdA). A levanase-encoding gene ( lsdB), starting 51 bp downstream of the lsdA gene, was cloned from strain SRT4. The lsdB gene (1605 bp) encodes a protein (calculated molecular mass 58.4 kDa) containing a putative 36-amino-acid s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0044-2
更新日期:2002-07-01 00:00:00
abstract::In the present work, Aspergillus fumigatus is described as a higher producer of hydrolytic enzymes secreted in response to the presence of the Callosobruchus maculatus bruchid pest. This fungus was able to grow over cowpea weevil shells as a unique carbon source, secreting alkaline proteolytic and chitinolytic enzymes...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0192-x
更新日期:2006-06-01 00:00:00
abstract::Protease production by Streptomyces sp. 594 was obtained after submerged fermentation (SF) and solid-state fermentation (SSF) using feather meal (FM) and corn steep liquor (CSL) as sole sources of carbon and nitrogen. Enzyme productions were 13.4 U ml(-1) in SF and 21.5 U g(-1) in SSF; these values were approximately ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0163-x
更新日期:2006-10-01 00:00:00
abstract::Unicellular green algae, like Chlorella, offer a potentially useful system for the expression of heterologous proteins. However, the development of Chlorella as a bioreactor has been delayed owing to the lack of a stable transformation technique. Here we report on the use of micro-projectile bombardment to introduce t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900268
更新日期:1997-12-01 00:00:00
abstract::Biofouling and microbiologically influenced corrosion are processes of material deterioration that originate from the attachment of microorganisms as quickly as the material is immersed in a nonsterile environment. Stainless steels, despite their wide use in different industries and as appliances and implant materials...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0193-4
更新日期:2006-12-01 00:00:00
abstract::The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P29...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1402-z
更新日期:2018-04-01 00:00:00
abstract::Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8(T) under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemastertrade mark 2D Platinum software. The molecular masses of the majorit...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0221-4
更新日期:2007-01-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis (SE) infection in humans is often associated with the consumption of contaminated poultry products. Binding of the bacterium to the intestinal mucosa is a major pathogenic mechanism of Salmonella in poultry. Transposon mutagenesis identified flgC as a potential binding mutant of...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0521-z
更新日期:2014-05-01 00:00:00
abstract::A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01577445
更新日期:1994-11-01 00:00:00
abstract::Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli....
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9537-1
更新日期:2010-04-01 00:00:00