Characteristics of the sigK Deletion Mutant from Bacillus thuringiensis var. israelensis Strain Bt-59.

Abstract:

:All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A sigK deletion mutant of B. thuringiensis var. israelensis strain Bt-59, Bt59(ΔsigK), was constructed by homologous recombination and characterized. The sigK deletion resulted in no mature spores and delayed mother cell lysis from T25 to T60, while the genetically complemented strain, Bt59(HFsigK), had mother cell lysis at T25. Compared to Bt-59, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated that the expression of Cry4Aa2/4Ba1 and Cyt1Aa1 proteins in Bt59(ΔsigK) increased approximately 1.67 and 1.21 times, respectively. However, there was no significant change in Cry11Aa1 protein expression between the two strains. Bioassay results showed that the sigK deletion mutation slightly reduced the insecticidal activity of Bt-59 against Culex pipiens pallens and did not obviously affect activity against Aedes albopictus.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Xu L,Han G,Fan X,Lv J,Zhang X,Peng Q,Zhang J,Xu J,Song F

doi

10.1007/s00284-020-02150-9

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

3422-3429

issue

11

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-020-02150-9

journal_volume

77

pub_type

杂志文章
  • Intracellular proteases of Bacillus thuringiensis subsp. kurstaki and a protease-deficient mutant Btk-q.

    abstract::The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3767-9

    authors: Reddy YC,Venkateswerlu G

    更新日期:2002-12-01 00:00:00

  • Species Diversity and Functional Prediction of Soil Bacterial Communities in Constructed Wetlands with Different Plant Conditions.

    abstract::The diversities of soil bacterial communities in the Phragmites australis, Phalaris arundinacea, and non-plantation constructed wetlands were compared and analyzed by through high-throughput Illumina sequencing. At the phylum level, the ten dominant bacterial phyla in the three types of constructed wetlands were the s...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01634-7

    authors: Fu W,Wang Y,Wei W,Li P

    更新日期:2019-03-01 00:00:00

  • Planifilum caeni sp. nov., a novel member of thermoactinomycete isolated from sludge compost.

    abstract::A Gram positive, thermophilic, and filamentous bacterium, designated strain GIESS004(T), was isolated from a sludge compost from Guangzhou, China. Growth occurred at 45-55 °C (optimum 50-53 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). The organism formed cream-yellow colonies with radial wrinkles. Aerial mycelium was not...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0693-6

    authors: Yu Z,Wu C,Yang GQ,Zhou SG

    更新日期:2015-01-01 00:00:00

  • Improved artificial saliva for studying the cariogenic effect of carbohydrates.

    abstract::Saliva is a complex fluid that possesses many important functions regarding oral health. Many in vitro studies require relatively large quantities of saliva. While natural saliva would be the material of choice, it is difficult to obtain in sufficient quantities and varies in composition. Substitutes mimicking the phy...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-9937-x

    authors: Björklund M,Ouwehand AC,Forssten SD

    更新日期:2011-07-01 00:00:00

  • Aureimonas leprariae sp. nov., Isolated from a Lepraria sp. Lichen.

    abstract::A Gram-negative, motile, aerobic and coccoid rod-shaped bacterium, designated strain YIM132180T, was isolated from a Lepraria sp. lichen collected from Pu'er, Yunnan Province, China. The strain grew at 15-35 °C (optimum, 25-28 °C), at 0-2% (w/v) NaCl (optimum, 0-1%) and at pH 6.0-9.0 (optimum, pH 7.0). The 16S rRNA ge...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01826-1

    authors: Zhang K,Jiang LQ,Wang LS,An DF,Lang L,Li GD,Wang XY,Shi SB,Li QY,Jiang CL,Jiang Y

    更新日期:2020-02-01 00:00:00

  • Stratified communities of active archaea in shallow sediments of the Pearl River Estuary, Southern China.

    abstract::Marine subsurface sediments represent a novel archaeal biosphere with unknown physiology. To get to know the composition and ecological roles of the archaeal communities within the sediments of the Pearl River Estuary, Southern China, the diversity and vertical distribution of active archaea in a sediment core were ch...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0320-y

    authors: Chen J,Wang F,Jiang L,Yin X,Xiao X

    更新日期:2013-07-01 00:00:00

  • AbrB and Spo0E control the proper timing of sporulation in Bacillus subtilis.

    abstract::We have shown previously that Spo0A approximately P-dependent sinIR operon expression was substantially down-regulated in abrB null mutant backgrounds. In this report, we show that loss of function mutations in abrB also cause phosphorelay gene expression to be down regulated. abrB null mutations caused diminished veg...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-003-4186-2

    authors: Shafikhani SH,Leighton T

    更新日期:2004-04-01 00:00:00

  • Abilities of Co-cultures of Brown-Rot Fungus Fomitopsis pinicola and Bacillus subtilis on Biodegradation of DDT.

    abstract::DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) is one of the pesticides that are hazardous for the environment and human health. Effective environmental-friendly treatment using co-cultures of fungi and bacteria is needed. In this study, the bacteria Bacillus subtilis at various volumes of 1, 3, 5, 7, and 10 mL (...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1286-y

    authors: Sariwati A,Purnomo AS,Kamei I

    更新日期:2017-09-01 00:00:00

  • Isolation and characterization of new cyclohexylacetic acid-degrading bacteria.

    abstract::Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9158-0

    authors: Iwaki H,Nakai E,Nakamura S,Hasegawa Y

    更新日期:2008-08-01 00:00:00

  • pH-dependent modulation of alkaline phosphatase activity in Serratia marcescens.

    abstract::Serratia marcescens is an opportunistic pathogen responsible for causing nosocomial infections, corneal ulcer, necrotizing fasciitis, cellulites, and brain abscess. Alkaline phosphatase (APase) is believed to play an important role in the survival of several intracellular pathogens and their adaptation. We have studie...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3740-7

    authors: Bhatti AR,Alvi A,Walia S,Chaudhry GR

    更新日期:2002-10-01 00:00:00

  • Increased diversity of predacious Bdellovibrio-like organisms (blos) as a function of eutrophication in Kumaon Lakes of India.

    abstract::Predation by Bdellovibrio-like organisms (BLOs) results in bacterial community succession in aquatic ecosystems. The effects of nutrient loading on the distribution and phylogeny of BLOs remain largely unknown. To this end, we present our findings on BLO diversity from four north-Indian lakes that are variable in thei...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9385-z

    authors: Chauhan A,Fortenberry GZ,Lewis DE,Williams HN

    更新日期:2009-07-01 00:00:00

  • Identification and Analysis of a Novel Gene Cluster Involves in Fe2+ Oxidation in Acidithiobacillus ferrooxidans ATCC 23270, a Typical Biomining Acidophile.

    abstract::Iron-oxidizing Acidithiobacillus spp. are applied worldwide in biomining industry to extract metals from sulfide minerals. They derive energy for survival through Fe2+ oxidation and generate Fe3+ for the dissolution of sulfide minerals. However, molecular mechanisms of their iron oxidation still remain elusive. A nove...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1453-9

    authors: Ai C,Liang Y,Miao B,Chen M,Zeng W,Qiu G

    更新日期:2018-07-01 00:00:00

  • Changes in bacterial communities accompanied by aggregation in a fed-batch composting reactor.

    abstract::The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9107-y

    authors: Watanabe K,Nagao N,Toda T,Kurosawa N

    更新日期:2008-05-01 00:00:00

  • Antibacterial effects of green tea polyphenols on clinical isolates of methicillin-resistant Staphylococcus aureus.

    abstract::The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patie...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9239-0

    authors: Cho YS,Schiller NL,Oh KH

    更新日期:2008-12-01 00:00:00

  • Investigation of the Cry4B-prohibitin interaction in Aedes aegypti cells.

    abstract::Bacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, and chikungunya. Previous work has shown that Cry4B binds to sever...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-012-0178-4

    authors: Kuadkitkan A,Smith DR,Berry C

    更新日期:2012-10-01 00:00:00

  • Impact of an herbicide combination of bromoxynil and prosulfuron on soil microorganisms.

    abstract::Soil microcosm experiments were used to investigate the effects on growth and activity of soil microorganisms of an herbicide combination (60% bromoxynil + 3% prosulfuron) frequently used to provide a broad spectrum control of weed species. Culturable aerobic bacteria, fungi, and actinomycetes, the fundamental groups ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0116-4

    authors: Pampulha ME,Oliveira A

    更新日期:2006-09-01 00:00:00

  • Regulation of alternative oxidase at the transcription stage in Aspergillus niger under the conditions of citric acid production.

    abstract::The citric acid-producing fungus Aspergillus niger WU-2223L possesses a cyanide-insensitive respiratory pathway catalyzed by alternative oxidase. The regulation of the alternative oxidase under the conditions of citric acid production was determined from the transcription level of the alternative oxidase gene (aox1). ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9369-z

    authors: Hattori T,Kino K,Kirimura K

    更新日期:2009-04-01 00:00:00

  • Characterization and transcription of the genes encoding enzymes involved in butyrate production in Butyrivibrio fibrisolvens.

    abstract::Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not pr...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3976-2

    authors: Asanuma N,Kawato M,Ohkawara S,Hino T

    更新日期:2003-09-01 00:00:00

  • Age-Related Changes in the Composition of Gut Bifidobacterium Species.

    abstract::Bifidobacteria are one of the major components in human microbiota that are suggested to function in maintaining human health. The colonization and cell number of Bifidobacterium species in human intestine vary with ageing. However, sequential changes of Bifidobacterium species ranging from newborns to centenarians re...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1272-4

    authors: Kato K,Odamaki T,Mitsuyama E,Sugahara H,Xiao JZ,Osawa R

    更新日期:2017-08-01 00:00:00

  • Antagonism Against Fish Pathogens by Cellular Components/Preparations of Bacillus coagulans (MTCC-9872) and It's In Vitro Probiotic Characterisation.

    abstract::Bacterial fish pathogens are pervasive in aquaculture. Control of bacterial fish pathogen is a difficult task among aquaculture practitioners. A large number of antibiotics are used for the control of prevalent bacterial pathogens in aquaculture. This may lead to drug resistance among pathogens and further treatment w...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1506-0

    authors: Midhun SJ,Neethu S,Vysakh A,Radhakrishnan EK,Jyothis M

    更新日期:2018-09-01 00:00:00

  • Isolation and Characterization of a Novel Bacteriophage Infecting Carbapenem-Resistant Klebsiella pneumoniae.

    abstract::A novel virulent phage, vB_KpnP_IME337, isolated from a hospital sewage in Beijing, China, that infects carbapenem-resistant Klebsiella pneumoniae KN2 capsular type was identified and characterized. Next-generation sequencing and genome analysis revealed that vB_KpnP_IME337 had a linear double-stranded genome with a l...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01849-8

    authors: Gao M,Wang C,Qiang X,Liu H,Li P,Pei G,Zhang X,Mi Z,Huang Y,Tong Y,Bai C

    更新日期:2020-05-01 00:00:00

  • Identification of Cross Reactive Antigens of C. botulinum Types A, B, E & F by Immunoproteomic Approach.

    abstract::Diseases triggered by microorganisms can be controlled by vaccines, which need neutralizing antigens. Hence, it is very crucial to identify extremely efficient immunogens for immune prevention. Botulism, a fatal neuroparalytic disease, is caused by botulinum neurotoxins produced by the anaerobic, Gram-positive spore-f...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1413-9

    authors: Sharma A,Ponmariappan S,Sarita R,Alam SI,Kamboj DV,Shukla S

    更新日期:2018-05-01 00:00:00

  • Cellular responses required for oxidative stress tolerance, colonization, and lesion formation by the necrotrophic fungus Alternaria alternata in citrus.

    abstract::The pathogenic capability of the tangerine pathotype of Alternaria alternata relies on the production of host-selective ACT toxin. Inoculation of A. alternata in leaves of the citrus quickly induced rapid lipid peroxidation, accumulation of hydrogen peroxide (H(2)O(2)), and cell death, indicative of host defensive res...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9795-y

    authors: Lin CH,Yang SL,Chung KR

    更新日期:2011-03-01 00:00:00

  • Correction to: Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers.

    abstract::The authors would like to correct the errors in the publication of the original article. ...

    journal_title:Current microbiology

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s00284-020-01947-y

    authors: Neveling DP,Ahire JJ,Laubscher W,Rautenbach M,Dicks LMT

    更新日期:2020-08-01 00:00:00

  • Cloning and expression of the insecticidal crystal protein gene Cry1Ca9 of Bacillus thuringiensis G10-01A from Taiwan granaries.

    abstract::A new cry gene (cry1Ca9) was cloned and sequenced from a Bacillus thuringiensis isolate native to Taiwan (G10-01A). The cry1C-type gene, designated cry1Ca9, consisted of an open reading frame of 3,567 bp, encoding a protein of 1,189 amino acid residues. The polypeptide has the deduced amino acid sequences predicting m...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3982-4

    authors: Kao SS,Hsieh FC,Tzeng CC,Tsai YS

    更新日期:2003-10-01 00:00:00

  • Diversity and symbiotic effectiveness of indigenous rhizobia-nodulating Adesmia bicolor in soils of Central Argentina.

    abstract::Native perennial legume Adesmia bicolor reveals characteristics that are key to securing persistence under grazing. Literature on the diversity and symbiotic effectiveness of indigenous rhizobia-nodulating A. bicolor in central Argentina is limited. The purpose of this study was therefore to determine phenotypic and g...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-012-0260-y

    authors: Bianco L,Angelini J,Fabra A,Malpassi R

    更新日期:2013-02-01 00:00:00

  • Complete Genome Sequences of Leclercia sp. W6 and W17 Isolated from a Gastric Cancer Patient.

    abstract::Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02075-3

    authors: Xu YY,Huang CJ,Xu L,Jiang XW,Xu XW,Xu XW

    更新日期:2020-10-01 00:00:00

  • Photonic Potential of Haloarchaeal Pigment Bacteriorhodopsin for Future Electronics: A Review.

    abstract::Haloarchaea are known for its adaptation in extreme saline environment. Halophilic archaea produces carotenoid pigments and proton pumps to protect them from extremes of salinity. Bacteriorhodopsin (bR) is a light-driven proton pump that resides in the membrane of haloarchaea Halobacterium salinarum. The photocycle of...

    journal_title:Current microbiology

    pub_type: 杂志文章,评审

    doi:10.1007/s00284-017-1271-5

    authors: Ashwini R,Vijayanand S,Hemapriya J

    更新日期:2017-08-01 00:00:00

  • The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis.

    abstract::Trioxacarcin A is a polyoxygenated, structurally complex antibiotic produced by Streptomyces spp., which possesses high anti-bacterial, anti-malaria, and anti-tumor activities. The trioxacarcin biosynthetic pathway involves type II polyketide synthases (PKSs) with L-isoleucine as a unique starter unit, as well as many...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0868-9

    authors: Yang K,Qi LH,Zhang M,Hou XF,Pan HX,Tang GL,Wang W,Yuan H

    更新日期:2015-10-01 00:00:00

  • Formic acid and acetic acid induce a programmed cell death in pathogenic Candida species.

    abstract::Cutaneous fungal infections are common and widespread. Antifungal agents used for the treatment of these infections often have undesirable side effects. Furthermore, increased resistance of the microorganisms to the antifungal drugs becomes the growing problem. Accordingly, the search for natural antifungal compounds ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0585-9

    authors: Lastauskienė E,Zinkevičienė A,Girkontaitė I,Kaunietis A,Kvedarienė V

    更新日期:2014-09-01 00:00:00