Isolation and Characterization of a Novel Bacteriophage Infecting Carbapenem-Resistant Klebsiella pneumoniae.

abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...

journal_title：Current microbiology

authors： Kim HE,Lee IS,Kim JH,Hahn KW,Park UJ,Han HS,Park KR

更新日期：2003-04-01 00:00:00

abstract：:The outer membrane protein of Photobacterium damsela (OMP-PD) and the gene encoding for this porin protein were isolated and characterized. The deduced amino acid sequence of the OMP-PD monomer has 338 amino acids and a calculated molecular weight of 36,951 Da. This sequence includes a 22-amino acid signal peptide at ...

journal_title：Current microbiology

authors： Gribun A,Katcoff DJ,Hershkovits G,Pechatnikov I,Nitzan Y

更新日期：2004-03-01 00:00:00

abstract：:Oxylipin metabolism represents one of the important hormonal and defensive mechanisms employed by plants, algae, or animals. It begins mostly with the reaction of lipoxygenases (LOXs), which catalyze the oxygenation of polyunsaturated fatty acids to form the corresponding hydroperoxides. At present, little information...

journal_title：Current microbiology

authors： Koeduka T,Kajiwara T,Matsui K

更新日期：2007-04-01 00:00:00

abstract：:Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleoti...

journal_title：Current microbiology

authors： Yokoyama E,Hirai S,Ishige T,Murakami S

更新日期：2017-04-01 00:00:00

abstract：:Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and construct...

journal_title：Current microbiology

authors： Lian C,Zhou Y,Feng F,Chen L,Tang Q,Yao Q,Chen K

更新日期：2014-04-01 00:00:00

abstract：:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...

journal_title：Current microbiology

authors： Zheng D,Valdez-Cruz NA,Armengol G,Sevrez C,Munoz-Olaya JM,Yuan Z,Orduz S,Crickmore N

更新日期：2007-01-01 00:00:00

abstract：:The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...

journal_title：Current microbiology

authors： Garcia M,Procópio L

更新日期：2020-06-01 00:00:00

abstract：:AVPDS (Animal Viruses Probe dataset) is a dataset of virus-specific and conserve oligonucleotides for identification and diagnosis of viruses infecting animals. The current dataset contain 20,619 virus specific probes for 833 viruses and their subtypes and 3,988 conserved probes for 146 viral genera. Dataset of virus ...

journal_title：Current microbiology

authors： Yadav BS,Pokhriyal M,Vasishtha DP,Sharma B

更新日期：2014-03-01 00:00:00

abstract：:Thirty-one Listeria strains were tested for sensitivity to four class IIa bacteriocins, namely, enterocin A, mesentericin Y105, divercin V41, and pediocin AcH, and to nisin A. Class IIa bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, wherea...

journal_title：Current microbiology

authors： Ennahar S,Deschamps N,Richard J

更新日期：2000-07-01 00:00:00

abstract：:Zygomycetes such as Cunninghamella elegans seem to be promising biosorbents for pollutants removal from wastewaters because of their particular cell wall characteristics. In this article the effect of ten culture media on C. elegans biomass composition was investigated by means of Fourier transform infra red spectrosc...

journal_title：Current microbiology

authors： Tigini V,Prigione V,Donelli I,Freddi G,Varese GC

更新日期：2012-01-01 00:00:00

abstract：:A common activity in the global search for useful Cry toxins is the microscopic screening of bacterial colonies for the presence of Bacillus thuringiensis. High-throughput screens require that aliquots from large numbers of colonies be arrayed on a microscopic slide. However, precisely placing a small amount of bacter...

journal_title：Current microbiology

authors： Ammons D,Rampersad J

更新日期：2015-09-01 00:00:00

abstract：:Bacteria acquire new DNA in a process known as horizontal gene transfer (HGT). To investigate the evolutionary impact of this transfer of DNA, various methods have been developed to detect past HGT events. For example, codon usage-based methods detect the presence of transferred genes by identifying atypical patterns ...

journal_title：Current microbiology

authors： Friedman R,Ely B

更新日期：2012-11-01 00:00:00

abstract：:The aim of this study is to investigate the combination of cefepime and sulbactam. Sulbactam, when administered , will effectively inhibit all Extended-spectrum beta lactamases (ESBLs) of the microorganism, while cefepime will inhibit the growth of the resistant microorganisms since it will not be hydrolyzed by OXA-48...

journal_title：Current microbiology

authors： Mataracı-Kara E,Yılmaz M,Özbek-Çelik B

更新日期：2020-09-01 00:00:00

abstract：:Microbial populations in indoor environments, where we live and eat, are important for public health. Various bacterial species reside in the kitchen, and refrigerators, the major means of food storage within kitchens, can be a direct source of food borne illness. Therefore, the monitoring of microbiota in the refrige...

journal_title：Current microbiology

authors： Jeon YS,Chun J,Kim BS

更新日期：2013-11-01 00:00:00

abstract：:Rhizobium sp. SIN-1, a nitrogen-fixing symbiont of Sesbania aculeata and other tropical legumes, carries two copies of nodD, both on a sym plasmid. We have isolated these two nodD genes by screening a genomic library of Rhizobium sp. SIN-1 with a nodD probe from Sinorhizobium meliloti. Nucleotide sequence and the dedu...

journal_title：Current microbiology

authors： Rana D,Krishnan HB

更新日期：2002-11-01 00:00:00

abstract：:The production and its potential use of a novel trihydroxy unsaturated fatty acid, 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD), were investigated. TOD was formed by Pseudomonas aeruginosa PR3 (NRRL B-18602) in a culture supplied with exogenous ricinoleic acid. The yield of TOD production was always higher in a ric...

journal_title：Current microbiology

authors： Kuo TM,Kim H,Hou CT

更新日期：2001-09-01 00:00:00

abstract：:Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsie...

journal_title：Current microbiology

authors： Lakshmi CV,Kumar M,Khanna S

更新日期：2009-01-01 00:00:00

abstract：:Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observe...

journal_title：Current microbiology

authors： Novotná J,Li XM,Novotná J,Vohradský J,Weiser J

更新日期：1995-08-01 00:00:00

abstract：:The psychrotrophic bacterium Aeromonas hydrophila 7966 was subjected to cold shocks from 30 degrees C to 20 degrees C, 15 degrees C, 10 degrees C, or 5 degrees C, or were incubated at low temperature to determine its adaptative response. The cell protein patterns analyzed by two-dimensional electrophoresis revealed th...

journal_title：Current microbiology

authors： Imbert M,Gancel F

更新日期：2004-08-01 00:00:00

abstract：:On the basis of the pulsed field gel electrophoresis(PFGE) technique, molecular karyotypes in Amoeboaphelidiumprotococcarum (the endotrophic parasite of chlorophycean algae, whichcombines protozoon- and fungus-type characters) were determined. Molecularkaryotypes in the strains X1, X5, and X31, which differ in the hos...

journal_title：Current microbiology

authors： Pinevich A,Gromov B,Mamkaeva K,Nasonova E

更新日期：1997-02-01 00:00:00

abstract：:The DNA sequence was determined for a region upstream of the puf operon of Rhodospirillum rubrum. A partial ORF was identified. The DNA sequence and the inferred amino acid sequences were aligned with those of bchA of Rhodobacter capsulatus and other phototrophic bacteria. Based on this alignment and genetic evidence,...

journal_title：Current microbiology

authors： Lee IY,Collins ML

更新日期：1993-08-01 00:00:00

abstract：:Native perennial legume Adesmia bicolor reveals characteristics that are key to securing persistence under grazing. Literature on the diversity and symbiotic effectiveness of indigenous rhizobia-nodulating A. bicolor in central Argentina is limited. The purpose of this study was therefore to determine phenotypic and g...

journal_title：Current microbiology

authors： Bianco L,Angelini J,Fabra A,Malpassi R

更新日期：2013-02-01 00:00:00

abstract：:Cutaneous fungal infections are common and widespread. Antifungal agents used for the treatment of these infections often have undesirable side effects. Furthermore, increased resistance of the microorganisms to the antifungal drugs becomes the growing problem. Accordingly, the search for natural antifungal compounds ...

journal_title：Current microbiology

authors： Lastauskienė E,Zinkevičienė A,Girkontaitė I,Kaunietis A,Kvedarienė V

更新日期：2014-09-01 00:00:00

abstract：:Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and posse...

journal_title：Current microbiology

authors： Yang H,Deng J,Yuan Y,Fan D,Zhang Y,Zhang R,Han B

更新日期：2015-02-01 00:00:00

abstract：:Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D, and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were r...

journal_title：Current microbiology

authors： Monnerat R,Masson L,Brousseau R,Pusztai-Carey M,Bordat D,Frutos R

更新日期：1999-09-01 00:00:00

abstract：:To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and...

journal_title：Current microbiology

authors： Liu H,Bao X

更新日期：2009-03-01 00:00:00

abstract：:Termites are global pests and can cause serious damage to buildings, crops, and plantation forests. The symbiotic intestinal flora plays an important role in the digestion of cellulose and nitrogen in the life of termites. Termites and their symbiotic microbes in the gut form a synergistic system. These organism work ...

journal_title：Current microbiology

authors： Zhou J,Duan J,Gao M,Wang Y,Wang X,Zhao K

更新日期：2019-06-01 00:00:00

abstract：:Corrinoids in several diverse species of methanogens were quantified by a bioassay utilizingEscherichia coli 113-3, a corrinoid auxotroph. All five species examined contained >0.65 nmol corrinoid/mg dry cells when grown on H2/CO2 as carbon and energy source. The highest corrinoid levels (4.1 nmol/mg cells) were found ...

journal_title：Current microbiology

authors： Krzycki J,Zeikus JG

更新日期：1980-07-01 00:00:00

abstract：:The first cloning and characterization of the gene katA, encoding the major catalase (KatA), from Xanthomonas is reported. A reverse genetic approach using a synthesized katA-specific DNA probe to screen a X. campestris pv. phaseoli genomic library was employed. A positively hybridizing clone designated pKat29 that co...

journal_title：Current microbiology

authors： Chauvatcharin N,Vattanaviboon P,Switala J,Loewen PC,Mongkolsuk S

更新日期：2003-02-01 00:00:00

abstract：:An aerobic, gram-reaction-negative, rod-shaped bacterium, designated strain Z6(T), was isolated from sediment collected at Mohe Basin, China. And its taxonomic position was investigated by applying a polyphasic approach. Growth occurs at 10-45 °C (optimum, 30 °C), at pH 6.0-11.0 (optimum, pH 7.0) and in the presence o...

journal_title：Current microbiology

authors： Han L,Teng YW,Feng QQ,Zhang RG,Zhao XM,Lv J,Xie B

更新日期：2016-09-01 00:00:00