Two novel exopolysaccharides from Bacillus amyloliquefaciens C-1: antioxidation and effect on oxidative stress.

Abstract:

:Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and possessed a molecular weight of 79.6 kDa, while EPS-2 contained only glucose and mannose in a 3:1 ratio, with the molecular weights of 19.8 kDa. The antioxidant activity results showed that EPS-1 exhibited strong reducing power, superoxide radicals (O(2-)·), and hydroxyl free radicals (OH·) scavenging activities. For the H2O2-induced injury in HepG2 cells, EPS-1 significantly decreased the formation of reactive oxygen species, intracellular malondialdehyde levels, and restored intracellular superoxide dismutase activity. For EPS-2, there had no detectable antioxidant activities. And all these results collectively showed that as a natural antioxidant, only EPS-1 produced by C-1 had considerable potential to be used as medical compounds or functional additives.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Yang H,Deng J,Yuan Y,Fan D,Zhang Y,Zhang R,Han B

doi

10.1007/s00284-014-0717-2

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

298-306

issue

2

eissn

0343-8651

issn

1432-0991

journal_volume

70

pub_type

杂志文章
  • An effective method to extract DNA from environmental samples for polymerase chain reaction amplification and DNA fingerprint analysis.

    abstract::A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF01577445

    authors: Porteous LA,Armstrong JL,Seidler RJ,Watrud LS

    更新日期:1994-11-01 00:00:00

  • Investigation of the Cry4B-prohibitin interaction in Aedes aegypti cells.

    abstract::Bacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, and chikungunya. Previous work has shown that Cry4B binds to sever...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-012-0178-4

    authors: Kuadkitkan A,Smith DR,Berry C

    更新日期:2012-10-01 00:00:00

  • Molecular Phylogeny and Taxonomy of Yamadazyma dushanensis f.a., sp. nov., a Cellobiose-Fermenting Yeast Species from China.

    abstract::Three yeast strains of Yamadazyma dushanensis f.a., sp. nov. were isolated from rotten wood samples collected in the Dushan Forest Park, Nanyang, Henan Province, China. Sequence analyses of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that this new species...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0847-1

    authors: Wang Y,Ren YC,Li Y,Hui FL

    更新日期:2015-08-01 00:00:00

  • Whole-Genome Analysis of an Extensively Drug-Resistance Empedobacter falsenii Strain Reveals Distinct Features and the Presence of a Novel Metallo-ß-Lactamase (EBR-2).

    abstract::The spread of antibiotic resistance is rapidly threatening the effectiveness of antibiotics in the clinical setting. Many infections are being caused by known and unknown pathogenic bacteria that are resistant to many or all antibiotics currently available. Empedobacter falsenii is a nosocomial pathogen that can cause...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1498-9

    authors: Collins C,Almuzara M,Saigo M,Montaña S,Chiem K,Traglia G,Mussi MA,Tolmasky M,Iriarte A,Vay C,Ramirez MS

    更新日期:2018-08-01 00:00:00

  • Bacterial growth on coral mucus.

    abstract::Coral mucus-degrading bacteria were isolated by an enrichment culture procedure. The isolates were able to grow as pure cultures on 10% sterilized mucus in seawater, yielding 10(8) CFU/ml. The isolates, mostly Vibrio strains, were classified by classical and molecular methods. When carbon, nitrogen, and phosphorus com...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9100-5

    authors: Sharon G,Rosenberg E

    更新日期:2008-05-01 00:00:00

  • In Vitro Synergistic Effect and Mutant Prevention Concentrations of Cefepime Alone or in Combination with Sulbactam Against OXA-48-positive Klebsiella pneumoniae Isolates.

    abstract::The aim of this study is to investigate the combination of cefepime and sulbactam. Sulbactam, when administered , will effectively inhibit all Extended-spectrum beta lactamases (ESBLs) of the microorganism, while cefepime will inhibit the growth of the resistant microorganisms since it will not be hydrolyzed by OXA-48...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02094-0

    authors: Mataracı-Kara E,Yılmaz M,Özbek-Çelik B

    更新日期:2020-09-01 00:00:00

  • Escherichia coli O157 somatic antigen is present in an isolate of E. fergusonii.

    abstract::A bacterium that tested positive with antibodies specific for Escherichia coli O157 was isolated from beef during routine screening procedures. The bacterium was identified as E. fergusonii by biochemical testing and partial sequencing of 16S rRNA. The isolate was tested for the presence of genes encoding Shiga toxins...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-0447-6

    authors: Fegan N,Barlow RS,Gobius KS

    更新日期:2006-06-01 00:00:00

  • Identification and initial characterization of a putative Mycoplasma gallinarum leucine aminopeptidase gene.

    abstract::Aminopeptidases (APN) may play a role in host colonization of M. gallinarum. Characterization of endogenous APN activity suggests that the leucine APN (LAP) of M. gallinarum is a metallo-aminopeptidase activated by Mn2+ and is present in the cytosol and possibly associated with the inner leaflet of the membrane. A 1.3...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-003-4098-1

    authors: Wan X,Branton SL,Hanson LA,Pharr GT

    更新日期:2004-01-01 00:00:00

  • Effects of the dicarboxylic acids malate and fumarate on E. coli O157:H7 and Salmonella enterica typhimurium populations in pure culture and in mixed ruminal microorganism fermentations.

    abstract::The dicarboxylic acids malate and fumarate increase ruminal pH, reduce methane production, increase propionate and total volatile fatty acid (VFA) production, and reduce lactic acid accumulation in a manner similar to ionophores. These acids stimulate the ruminal bacterium Selenomonas ruminantium to ferment lactate to...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9351-1

    authors: Nisbet DJ,Callaway TR,Edrington TS,Anderson RC,Krueger N

    更新日期:2009-05-01 00:00:00

  • Mycobacterium tuberculosis expresses ftsE gene through multiple transcripts.

    abstract::Bacterial FtsE gene codes for the ATP-binding protein, FtsE, which in complex with the transmembrane protein, FtsX, participates in diverse cellular processes. Therefore, regulated expression of FtsE and FtsX might be critical to the human pathogen, Mycobacterium tuberculosis, under stress conditions. Although ftsX ge...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-9897-1

    authors: Roy S,Vijay S,Arumugam M,Anand D,Mir M,Ajitkumar P

    更新日期:2011-05-01 00:00:00

  • Presence of qacEΔ1 gene and susceptibility to a hospital biocide in clinical isolates of Pseudomonas aeruginosa resistant to antibiotics.

    abstract::Biocides play an important role in healthcare-associated infection control by either minimizing or preventing microorganism dissemination. This study evaluated the susceptibility of Pseudomonas aeruginosa clinical isolates to a quaternary ammonium (QAC) disinfectant and antibiotics, and verified the presence of qacEΔ1...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-9934-0

    authors: Romão C,Miranda CA,Silva J,Mandetta Clementino M,de Filippis I,Asensi M

    更新日期:2011-07-01 00:00:00

  • Use of Vine-Trimming Wastes as Carrier for Amycolatopsis sp. to Produce Vanillin, Vanillyl Alcohol, and Vanillic Acid.

    abstract::Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main fl...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-016-1094-9

    authors: Castañón-Rodríguez JF,Pérez-Rodríguez N,de Souza Oliveira RP,Aguilar-Uscanga MG,Domínguez JM

    更新日期:2016-10-01 00:00:00

  • Analysis of microbial population dynamics in a partial nitrifying SBR at ambient temperature.

    abstract::In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0317-6

    authors: Zeng T,Li D,Zeng H,Zhang Z,Liu L,Zhang X,Zhang J

    更新日期:2013-06-01 00:00:00

  • Bacillus thuringiensis protein Cry6B (BGSC ID 4D8) is toxic to larvae of Hypera postica.

    abstract::Insecticidal proteins produced by strains of Bacillus thuringenesis are specific toward target pests. One of the Bt proteins, Cry 1Ac has been used successfully for controlling crop predation by polyphagous pests Helicoverpa armigera. Structurally, Bt proteins consist of three domains; domain I and III are fairly homo...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9749-4

    authors: Sharma A,Kumar S,Bhatnagar RK

    更新日期:2011-02-01 00:00:00

  • Analysis of cell wall constituents of biocide-resistant isolates from dental-unit water line biofilms.

    abstract::In past years, the significance of microbial resistance to biocides has increased. Twenty biocide-resistant bacterial strains were isolated from dental-unit water line biofilm. All strains resisted high biocide concentrations (up to 100 microg ml(-1)): sodium dodecyl sulphate, hydrogen peroxide, sodium hypochlorite, p...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9200-2

    authors: Liaqat I,Sabri AN

    更新日期:2008-10-01 00:00:00

  • Cell division and trichome breakage inBeggiatoa.

    abstract::The process ofBeggiatoa trichome division was elucidated through phase-contrast microscopy and transmission and scanning electron microscopy. Trichome breakage and dispersion is accomplished by the formation of sacrificial cells (necridia) at various points within the trichome. Upon dying, the sacrificial cells lyse, ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF02601668

    authors: Strohl WR,Larkin JM

    更新日期:1978-01-01 00:00:00

  • Bacterial Diversity in Bohai Bay Solar Saltworks, China.

    abstract::The microbiota in solar salterns plays an important role in salt production quantitatively and qualitatively. Bohai Bay coast is the major sea salt producing area in China. However, few ecological characterization studies of the Bohai Bay salt ponds, particularly of their microbial diversity, have been conducted. This...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0916-5

    authors: Zhang J,Ma G,Deng Y,Dong J,Van Stappen G,Sui L

    更新日期:2016-01-01 00:00:00

  • The tracing of mycobacteria in drinking water supply systems by culture, conventional, and real time PCRs.

    abstract::Mycobacteria are widely present in diverse aquatic habitats, where they can survive for months or years while some species can even proliferate. The resistance of different mycobacterial species to disinfection methods like chlorination or ozonation could result in their presence in the final tap water of consumers. I...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0427-1

    authors: Klanicova B,Seda J,Slana I,Slany M,Pavlik I

    更新日期:2013-12-01 00:00:00

  • Comparative evaluation of five culture media with triplex PCR assay for detection of methicillin-resistant Staphylococcus aureus.

    abstract::The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is responsible for nosocomial and community-acquired infections. Hence, rapid and accurate laboratory diagnosis of MRSA is a vital constituent of control measures. The present study evaluated five different methods for the identification of MRSA. A to...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9567-8

    authors: Al-Talib H,Yean CY,Al-khateeb A,Singh KK,Hasan H,Al-Jashamy K,Ravichandran M

    更新日期:2010-07-01 00:00:00

  • P-27/HP endolysin as antibacterial agent for antibiotic resistant Staphylococcus aureus of human infections.

    abstract::Staphylococcus aureus causes a wide range of suppurative infections in humans and animals. Due to its high virulence, ability to adopt various environmental conditions, and acquired multiple drug resistance, treatment of such infections has become difficult. Therefore, there is an immense need to develop alternate dru...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-9939-8

    authors: Gupta R,Prasad Y

    更新日期:2011-07-01 00:00:00

  • Characterization of a New Ezakiella Isolated from the Human Vagina: Genome Sequence and Description of Ezakiella massiliensis sp. nov.

    abstract::The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P29...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1402-z

    authors: Diop K,Andrieu C,Michelle C,Armstrong N,Bittar F,Bretelle F,Fournier PE,Raoult D,Fenollar F

    更新日期:2018-04-01 00:00:00

  • Microbulbifer arenaceous sp. nov., a new endolithic bacterium isolated from the inside of red sandstone.

    abstract::An endolithic bacterium, strain RSBr-1, was isolated from the inside of a piece of red sandstone from coastal areas of Scotland. RSBr-1 was gram negative, oxidase and catalase positive, and cells were non-motile rods. Sodium was required for growth. The optimum sodium chloride concentration and pH for growth were 4% a...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-003-4006-8

    authors: Tanaka T,Yan L,Burgess JG

    更新日期:2003-11-01 00:00:00

  • Isolation and characterization of new cyclohexylacetic acid-degrading bacteria.

    abstract::Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9158-0

    authors: Iwaki H,Nakai E,Nakamura S,Hasegawa Y

    更新日期:2008-08-01 00:00:00

  • The luxS gene is involved in AI-2 production, pathogenicity, and some phenotypes in Erwinia amylovora.

    abstract::Erwinia amylovora causes fire blight of apple, pear, and other members of the Rosaceae family. The enzyme LuxS catalyzes the last step in the production of autoinducer-2 (AI-2), a molecule implicated with quorum sensing in many bacterial species. It is now well recognized that LuxS also plays a central role in sulfur ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9256-z

    authors: Gao Y,Song J,Hu B,Zhang L,Liu Q,Liu F

    更新日期:2009-01-01 00:00:00

  • NCW2, a Gene Involved in the Tolerance to Polyhexamethylene Biguanide (PHMB), May Help in the Organisation of β-1,3-Glucan Structure of Saccharomyces cerevisiae Cell Wall.

    abstract::In the present work, we provide biological evidences supporting the participation of NCW2 gene in the mechanism responsible for cell tolerance to polyhexamethylene biguanide (PHMB), an antifungal agent. The growth rate of yeast cells exposed to this agent was significantly reduced in ∆ncw2 strain and the mRNA levels o...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-016-1067-z

    authors: Elsztein C,de Lima RCP,de Barros Pita W,de Morais MA Jr

    更新日期:2016-09-01 00:00:00

  • Luteimonas weifangensis sp. nov., Isolated from Bensulfuron-Methyl Contaminated Watermelon Soil.

    abstract::A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02210-0

    authors: Zhang H,Zhao HZ,Liu J,Qin W,Huang X

    更新日期:2020-11-01 00:00:00

  • Sequencing, characterization, and gene expression analysis of the histidine decarboxylase gene cluster of Morganella morganii.

    abstract::The histidine decarboxylase gene cluster of Morganella morganii DSM30146(T) was sequenced, and four open reading frames, named hdcT1, hdc, hdcT2, and hisRS were identified. Two putative histidine/histamine antiporters (hdcT1 and hdcT2) were located upstream and downstream the hdc gene, codifying a pyridoxal-P dependen...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0490-7

    authors: Ferrario C,Borgo F,de Las Rivas B,Muñoz R,Ricci G,Fortina MG

    更新日期:2014-03-01 00:00:00

  • Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea.

    abstract::Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage tite...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0984-6

    authors: Li Y,Wang M,Liu Q,Song X,Wang D,Ma Y,Shao H,Jiang Y

    更新日期:2016-05-01 00:00:00

  • Detection of Diverse N-Acyl Homoserine Lactone Signalling Molecules Among Bacteria Associated with Rice Rhizosphere.

    abstract::Bacterial communities communicate, regulate and coordinate their cooperative activities and physiological process by releasing, sensing and responding to small diffusible signal molecules such as acyl homoserine lactones (AHLs), auto-inducing oligo-peptides (AIPs) and autoinducer-2, a process referred to as Quorum sen...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02183-0

    authors: Viswanath G,Sekar J,Ramalingam PV

    更新日期:2020-11-01 00:00:00

  • Lipase activity from strains of Pasteurella multocida.

    abstract::Thirteen clinical isolates of Pasteurella multocida from a variety of different animals and humans were examined for their ability to produce lipase. Lipase substrates used included Tween 20, Tween 40, Tween 80, and Tween 85. Lipase activity was detected in the filtrates of organisms grown to the exponential phase in ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849910061

    authors: Pratt J,Cooley JD,Purdy CW,Straus DC

    更新日期:2000-05-01 00:00:00