Abstract:
:In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study microbial population dynamics. Partial nitrification was effectively achieved in response to shifts of influent ammonium concentrations. DGGE results showed that higher ammonia concentration referred to lower ammonia-oxidizing bacteria (AOB) diversity in the SBR. Phylogenetic analysis revealed that all the predominant AOB was affiliated with Nitrosomonas genus. FISH analysis illustrated AOB was the predominant nitrifying bacteria of microbial compositions when SBR achieved partial nitrification (PN) at ambient temperature.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Zeng T,Li D,Zeng H,Zhang Z,Liu L,Zhang X,Zhang Jdoi
10.1007/s00284-013-0317-6subject
Has Abstractpub_date
2013-06-01 00:00:00pages
614-20issue
6eissn
0343-8651issn
1432-0991journal_volume
66pub_type
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