Analysis of microbial population dynamics in a partial nitrifying SBR at ambient temperature.

Abstract:

:In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study microbial population dynamics. Partial nitrification was effectively achieved in response to shifts of influent ammonium concentrations. DGGE results showed that higher ammonia concentration referred to lower ammonia-oxidizing bacteria (AOB) diversity in the SBR. Phylogenetic analysis revealed that all the predominant AOB was affiliated with Nitrosomonas genus. FISH analysis illustrated AOB was the predominant nitrifying bacteria of microbial compositions when SBR achieved partial nitrification (PN) at ambient temperature.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Zeng T,Li D,Zeng H,Zhang Z,Liu L,Zhang X,Zhang J

doi

10.1007/s00284-013-0317-6

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

614-20

issue

6

eissn

0343-8651

issn

1432-0991

journal_volume

66

pub_type

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