Abstract：
:Flagella occur on many prokaryotes, which primarily propel cells to move from detrimental to favorable environments. A variety of species-specific flagellation patterns have been identified. Although it is presumed that for each of these flagellated microorganisms, an evolutionarily fixed flagellation pattern is favored under the normal living conditions, direct evidence is lacking. Here, we use Shewanella oneidensis, a rod-shaped Gram-negative bacterium with a monotrichous polar flagellum (MR-1, the wild-type), as a research model. The investigation has been enabled by multiple mutants with diverse flagellation patterns that had been generated by removing FlhF and FlhG proteins that control flagellar location and number, respectively. Growth assays, as a measure of fitness, revealed that the wild-type strain predominated in spreading on swim plates and in pellicles which form at the air-liquid interface. However, under the pellicles where oxygen is limited, both aflagellated and monotrichous lateral strains showed similar increase in fitness, whereas strains with multiple flagella were less competitive. Moreover, under shaking culturing conditions, the aflagellated strain outcompeted all other strains, including the wild-type, suggesting that cells devoid of flagella would be more likely enriched upon agitation. Overall, these data support the presumption that the monotrichous polar flagellum, as evolutionarily fixed in the wild-type strain, is optimal for the growth fitness of S. oneidensis over any other mutants under most test conditions. However, upon specific changes of environmental conditions, another form could come to predominate. These findings provide insight into the impacts of flagellation patterns and function on bacterial adaptation to differing environments.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Yang RS,Chen YTdoi
10.1007/s00284-020-01999-0subject
Has Abstractpub_date
2020-08-01 00:00:00pages
1790-1799issue
8eissn
0343-8651issn
1432-0991pii
10.1007/s00284-020-01999-0journal_volume
77pub_type
杂志文章abstract：:Corynebacterium glutamicum, a Gram-positive soil bacterium belonging to the mycolic acids-containing actinomycetes, is able to use the lignin degradation products ferulate, vanillate, and protocatechuate as sole carbon sources. The gene cluster responsible for vanillate catabolism was identified and characterized. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4531-8
更新日期：2005-07-01 00:00:00
abstract：:The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. or...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1350-7
更新日期：2018-01-01 00:00:00
abstract：:(13)CNMR and (1)HNMR studies revealed that aluminum citrate (Al-citrate) was metabolized intracellularly and that oxalic acid was an important product in the Al-stressed cells. This dicarboxylic acid was produced via the oxidation of glyoxylate, a precursor generated through the cleavage of isocitrate. In the control ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3944-x
更新日期：2003-07-01 00:00:00
abstract：:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1217-y
更新日期：2017-04-01 00:00:00
abstract：:Minimal inhibitory concentrations (MICs) of five antibiotics namely amikacin, gentamicin, tetracycline, ciprofloxacin, and nitrofurantoin for pork isolates of Yersinia enterocolitica increased two- to eightfold after bacteria were grown in the presence of 5 mm arsenite. For Y. enterocolitica isolates obtained from was...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010277
更新日期：2001-08-01 00:00:00
abstract：:Antibiotics are generally applied for treatment or as subtherapeutic agents to overcome diseases caused by pathogenic bacteria including Escherichia coli, Salmonella and Enterococcus species in poultry. However, due to their possible adverse effects on animal health and to maintain food safety, probiotics, prebiotics,...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02091-3
更新日期：2020-09-01 00:00:00
abstract：:Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein wa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0570-3
更新日期：2014-08-01 00:00:00
abstract：:In Neurospora crassa, multinucleate macroconidia are used for genetic transformation. The barrier for such a transformation can be either at the cell membrane level or at the nuclear membrane level. For assessment of these possibilities, a forced heterokaryon (containing two genetically marked nuclei and auxotrophic f...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0004-x
更新日期：2002-05-01 00:00:00
abstract：:Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9158-0
更新日期：2008-08-01 00:00:00
abstract：:We report the in vitro phytopathogen suppression activity of siderophoregenic preparations of Alcaligenes feacalis vis-à-vis the oraganochlorine fungicide, bavistin. Siderophore-rich culture broth, siderophore-rich supernatant, and purified siderophore preparation exerted antifungal activity against Aspergillus niger ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9264-z
更新日期：2009-01-01 00:00:00
abstract：:This paper presents the results of studies on the activity of extra-cellular enzymes in soil-willow vegetation filter soil which is used in the post-treatment of household sewage in an onsite wastewater treatment system located in central Poland. Wastewater is discharged from the detached house by gravity into the ons...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0230-4
更新日期：2012-12-01 00:00:00
abstract：:Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage tite...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0984-6
更新日期：2016-05-01 00:00:00
abstract：:The 5' untranslated region and the orf1 sequence from the cry2Aa1 operon from Bacillus thuringiensis subsp. kurstaki NRD-12 were sequenced and compared to that from strain HD-1. The start codon described in HD-1 does not yield in NRD-12 a protein of the expected size of 20 kDa, but a 10-amino acid peptide. A second, h...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006828
更新日期：1999-07-01 00:00:00
abstract：:Culture-based analysis was employed in parallel with PCR amplification of 16S rDNA, coupled with denaturing gradient gel electrophoresis (DGGE), to profile bacterial species associated with different developmental stages of the pine false webworm (PFW), Acantholyda erythrocephala, a sawfly pest responsible for inciden...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9243-4
更新日期：2008-12-01 00:00:00
abstract：:NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00298381
更新日期：1995-10-01 00:00:00
abstract：:Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9386-y
更新日期：2009-06-01 00:00:00
abstract：:A 11.2-kb fragment containing the ectABC genes of the biosynthetic pathway of ectoine from the Gram-positive, moderately halophilic bacterium Halobacillus dabanensis D-8(T) was obtained by inverse polymerase chain reaction. Subsequently, the entire ectABC cluster was cloned and analyzed. It revealed that the intergeni...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0396-0
更新日期：2006-09-01 00:00:00
abstract：:Saliva is a complex fluid that possesses many important functions regarding oral health. Many in vitro studies require relatively large quantities of saliva. While natural saliva would be the material of choice, it is difficult to obtain in sufficient quantities and varies in composition. Substitutes mimicking the phy...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9937-x
更新日期：2011-07-01 00:00:00
abstract：:A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1553-6
更新日期：2018-11-01 00:00:00
abstract：:Probiotics are well known for their wide range of beneficial activities. However, recent use of probiotic Bifidobacterium, Enterococcus, and Lactobacillus spp. has been plagued by certain disadvantages such as complex growth requirements, high maintenance cost, susceptibility to the gastrointestinal environment, patho...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01647-2
更新日期：2019-04-01 00:00:00
abstract：:Two strains of the well-known insect pathogen and biopesticide, Bacillus thuringiensis (Bt), belonging to subspecies alesti (strain Bt5) and kurstaki (strain Bt213), were chosen for genetic characterization. The two strains belong to different serotypes and are currently classified into different subspecies, although ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0055-z
更新日期：2002-02-01 00:00:00
abstract：:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0352-7
更新日期：2007-01-01 00:00:00
abstract：:The relationship between 16S rRNA gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus Saprospira was determined. Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified 16S rRNA genes of these isolates indicated f...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4358-8
更新日期：2004-10-01 00:00:00
abstract：:A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0-8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular pola...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01823-4
更新日期：2020-02-01 00:00:00
abstract：:Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900082
更新日期：1996-08-01 00:00:00
abstract：:In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biof...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1080-2
更新日期：2016-10-01 00:00:00
abstract：:Genetic studies of Ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. A tightly regulated gene-expression system was developed for O. anthropi using the lacI(q) gene and a re-engineered coliphage T5...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9532-6
更新日期：2010-04-01 00:00:00
abstract：:Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus pr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3789-3
更新日期：2003-04-01 00:00:00
abstract：:Biofouling leads to water quality deterioration and higher maintenance cost for cleaning of membranes. The present study has demonstrated the application of a biomolecule (vanillin) in scrubbing and destabilizing biofilms of drinking water reverse osmosis (RO) membrane module in lab scale reactor set-up. Reverse osmos...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02003-5
更新日期：2020-09-01 00:00:00
abstract：:This report presents results from a study on the bacterial diversity of cigarette brands collected from military personnel during the U.S. Army's investigation of a series of cases of acute eosinophilic pneumonitis in military personnel deployed in Operation Iraqi Freedom. Eight species of Bacillus, including five new...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4491-z
更新日期：2005-07-01 00:00:00
