Abstract:
:In the present study, probiotic, safety and functional characteristics of eight indigenous bifidobacterial isolates were compared to identify suitable strains for functional food application. Among the isolates, six strains of Bifidobacterium longum and one each of Bifidobacterium breve and Bifidobacterium bifidum were identified by 16S rRNA, xfp and hsp60 gene sequencing. Diversity among these strains was established by RAPD and Rep-PCR. Genes associated with sortase-dependent pili (SDP) (credited for role in adhesion) and serpin (immunomodulation) which can serve as potential marker genes for rapid identification of probiotic Bifidobacterium, was also evaluated. All the isolates exhibited potential probiotic, functional (antimicrobial activity, antioxidant activity, phytase activity, milk fermentation ability) and safety attributes. However, among them, B. breve NCIM5671 exhibited, better tolerance to low pH, amylase activity and exopolysaccharide producing ability. B. bifidum NCIM5697 and B. longum NCIM5672 demonstrated higher adherence ability to Caco-2 cells. NCIM5697 also displayed exopolysaccharide producing ability while NCIM5672 showed strong antibacterial activity against pathogens tested. Further, with respect to presence of adhesion marker genes, disparity was observed among B. longum strains. B. longum NCIM5684 and B. longum NCIM5686 displayed presence of subunits of SDP reported to be present in B. breve. In addition, B. longum NCIM5686 also lacked SDP present in all other B. longum isolates. B. breve NCIM5671, B. longum NCIM5672 and B. bifidum NCIM5697 with appreciable traits qualifies as potential probiotic cultures. Further, the variations observed in molecular and functional characteristics of isolates signify genetic diversity among the cultures.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Achi SC,Halami PMdoi
10.1007/s00284-018-1615-9subject
Has Abstractpub_date
2019-03-01 00:00:00pages
304-311issue
3eissn
0343-8651issn
1432-0991pii
10.1007/s00284-018-1615-9journal_volume
76pub_type
杂志文章abstract::Marine subsurface sediments represent a novel archaeal biosphere with unknown physiology. To get to know the composition and ecological roles of the archaeal communities within the sediments of the Pearl River Estuary, Southern China, the diversity and vertical distribution of active archaea in a sediment core were ch...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0320-y
更新日期:2013-07-01 00:00:00
abstract::To date, a few works have performed a correlation of metabolic variables in bacteria; however specific correlations with these variables have not been reported. In this work, we included 36 human pathogenic bacteria and 18 non- or less-pathogenic-related bacteria and obtained all metabolic variables, including enzymes...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1013-0
更新日期:2016-06-01 00:00:00
abstract::A single strain, designated BF49(T), was isolated from a biofilm of a tufa deposit from the Westerhöfer rivulet, Lower Saxony, Germany. The G+C content of the genomic DNA of strain BF49(T) was 69 mol% and the predominant ubiquinone was Q-8. Major fatty acids were C(16:1)omega7c/15 iso 2OH and C(16:0). Comparative 16S ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9095-3
更新日期:2008-04-01 00:00:00
abstract::Several di-, tri-, and oligopeptides were incubated individually in vitro with rumen fluid from two sheep receiving a mixed grass hay/concentrate diet and with washed cells of Prevotella (formerly Bacteroides) ruminicola M384 and P. ruminicola B(1)4. The rates of breakdown of most peptides were similar in the rumen fl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01576265
更新日期:1993-06-01 00:00:00
abstract::Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900462
更新日期:1999-11-01 00:00:00
abstract::The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3767-9
更新日期:2002-12-01 00:00:00
abstract::In this article, we investigated the effect of exogenous calcium on improving viability of Debaryomyces hansenii and Pichia membranaefaciens under heat stress, and evaluated the role of calcium in reducing oxidant damage of proteins in the yeast cells. The results indicated that high concentration of exogenous calcium...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0133-4
更新日期:2012-08-01 00:00:00
abstract::The organization of the clusters of genes encoding proteins of the botulinum neurotoxin (BoNT) progenitor complex was elucidated in a strain of Clostridium botulinum producing type B and F neurotoxins. With PCR and sequencing strategies, the type B BoNT-gene cluster was found to be composed of genes encoding BoNT/B, n...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900384
更新日期:1998-11-01 00:00:00
abstract::Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900452
更新日期:1999-11-01 00:00:00
abstract::Two strains of Rhizobia isolated from nodules of Vicia faba var. major and one strain isolated from nodules of Cicer arietinum L. were characterized for salt resistance. The presence of 1 mM glycine betaine or choline in a minimal medium with added NaCl had a beneficial role on the growth of the three strains. Both mo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900163
更新日期:1997-03-01 00:00:00
abstract::We undertook the issue of the distribution of intracellular bacteria among Oribatida (Acari). Six genera of bacteria were detected by PCR and Sanger DNA sequencing: Wolbachia, Cardinium, Rickettsia, Spiroplasma, Arsenophonus, and Hamiltonella. Our research, for the first time, revealed the presence of Cardinium in Mic...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01717-5
更新日期:2019-09-01 00:00:00
abstract::Serratia marcescens is an opportunistic pathogen responsible for causing nosocomial infections, corneal ulcer, necrotizing fasciitis, cellulites, and brain abscess. Alkaline phosphatase (APase) is believed to play an important role in the survival of several intracellular pathogens and their adaptation. We have studie...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3740-7
更新日期:2002-10-01 00:00:00
abstract::Vegetative insecticidal protein (Vip3) from Bacillus thuringiensis shows high activity against lepidopteran insects. Cytolytic δ-endotoxin (Cyt) also has high toxicity to dipteran larvae and synergism with other crystal proteins (Cry), but synergism between Cyt and Vip3 proteins has not been tested. We analyzed for sy...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0070-7
更新日期:2012-04-01 00:00:00
abstract::The genus Acinetobacter has been recognized to take up exogenous DNA from the environment. In this study, we conducted natural transformation with a novel diesel-degrading Acinetobacter sp. strain, designated strain DR1, using the broad host range plasmid pRK415. Many factors, including temperature, quantities of DNA,...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9698-y
更新日期:2011-01-01 00:00:00
abstract::Antibiotics are generally applied for treatment or as subtherapeutic agents to overcome diseases caused by pathogenic bacteria including Escherichia coli, Salmonella and Enterococcus species in poultry. However, due to their possible adverse effects on animal health and to maintain food safety, probiotics, prebiotics,...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02091-3
更新日期:2020-09-01 00:00:00
abstract::Reindeer (Rangifer tarandus tarandus) may include large proportions of lichens in their winter diet. These dietary lichens are rich in phenolic secondary compounds, the most well-known being the antimicrobial usnic acid. Previous studies have shown that reindeer host rumen bacteria resistant to usnic acid and that usn...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0534-7
更新日期:2014-06-01 00:00:00
abstract::Pasteurella haemolytica was isolated from three of 18 grass samples and four of 18 water samples collected from two grazing fields occupied by sheep. This microorganism was also isolated from three of nine straw bedding samples collected from a pen housing ewes affected by mastitis caused by P. haemolytica. The same e...
journal_title:Current microbiology
pub_type: 新闻
doi:10.1007/s002849900261
更新日期:1997-11-01 00:00:00
abstract::An autolysis chitinase was purified from the cultural medium of the anaerobic fungus Piromyces communis OTS1 by ammonium sulfate precipitation, affinity chromatography with regenerated chitin, chromato-focusing, gel filtration, and chromato-focusing again. The optimal pH and temperature were 6.0 and 50 degrees C, resp...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900210
更新日期:1997-07-01 00:00:00
abstract::A new antagonistic Burkholderia strain, designated MP-1 and producing antifungal activities against various filamentous plant pathogenic fungi, was isolated from the rhizoshere in the Naju area. Cultural characteristic studies strongly suggested that this strain belongs to the genus Burkholderia. The nucleotide sequen...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0333-2
更新日期:2006-11-01 00:00:00
abstract::Biofilm-forming bacteria Pseudomonas aeruginosa is a common pathogen in mechanically ventilated newborns, which can cause life-threatening infections. Alginate of mucoid Pseudomonas aeruginosa biofilms is considered an important virulence factor which contributes to the resistance to antibiotics. Traditionally, ambrox...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9142-8
更新日期:2008-07-01 00:00:00
abstract::Magnetotactic bacteria (MTB) are capable of synthesizing nano-sized, intracellular membrane-bound magnetosomes. To learn more about the genetic factors involved in magnetosome formation, transposon mutagenesis was carried out by conjugation using a hyperactive mariner transposon to obtain nonmagnetic mutants of Magnet...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0131-6
更新日期:2012-07-01 00:00:00
abstract::A gram-negative bacterium, designated FJY1(T), was isolated from a soil sample obtained from a university campus in South Korea. Examination showed that FJY1(T) was red-pigmented, aerobic, motile, and composed of nonspore-forming rods. This strain grew in a temperature range of 15-37 °C and a pH range of 7-9. Based on...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1105-x
更新日期:2016-11-01 00:00:00
abstract::The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0295-z
更新日期:2007-03-01 00:00:00
abstract::Grasslands are suffering from long-term overgrazing because of the population inflation. Furthermore, nitrous oxide (N2O) is a major greenhouse gas that also depletes stratospheric ozone. However, the emission rate of grassland N2O and underlying mechanisms remained unclear under different grazing intensities. We cond...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01668-x
更新日期:2019-05-01 00:00:00
abstract::In laboratory cultures of ice nucleation-active (Ice+) Erwinia herbicola isolates, it has been difficult to achieve high-level expression of ice nuclei, especially nuclei active at temperatures warmer than -5 degrees C (i.e., type 1 ice nuclei). Here we demonstrate that starvation for phosphate and exposure to low tem...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900325
更新日期:1998-06-01 00:00:00
abstract::Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0009-z
更新日期:2011-12-01 00:00:00
abstract::The fungus Mycogone perniciosa is a major pathogen of the common button mushroom Agaricus bisporus. Analysis of genetic diversity in M. Perniciosa may assist in developing methods for prophylaxis and treatment of M. Perniciosa infections. For this, it is necessary to classify M. Perniciosa into relevant class groups q...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1020-1
更新日期:2016-07-01 00:00:00
abstract::Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9608-3
更新日期:2010-10-01 00:00:00
abstract::Campylobacter jejuni is a major food-borne pathogen that causes human gastroenteritis in many developed countries. In our study, we applied multilocus sequence typing (MLST) technology to 167 C. jejuni isolates from diverse sources in Eastern China to examine their genetic diversity. MLST defined 94 sequence types (ST...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0853-3
更新日期:2015-09-01 00:00:00
abstract::Triterpenoid saponin derivatives oleanolic acid (OA) and ursolic acid (UA), but not betulinic acid (BA), were previously found to have strong antimicrobial activity against Streptococcus mutans. OA and UA inhibited the transcription of genes related to peptidoglycan biosynthesis, thereby preventing bacterial growth. H...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1344-5
更新日期:2018-01-01 00:00:00