Abstract：
:The genus Acinetobacter has been recognized to take up exogenous DNA from the environment. In this study, we conducted natural transformation with a novel diesel-degrading Acinetobacter sp. strain, designated strain DR1, using the broad host range plasmid pRK415. Many factors, including temperature, quantities of DNA, and aeration have proven critically important for efficient natural transformation. Interestingly, the Acinetobacter sp. strain DR1 (pRK415) differed both phenotypically and physiologically from the wild-type strain in several regards, including motility, biofilm formation ability, and responses to oxidative stress: the transformed cells were rendered more sensitive to hydrogen peroxide and cumene hydroperoxide, and their motilities and biofilm formation activity were also attenuated. Our data demonstrated that caution should be exercised when conducting genetic manipulation with plasmids, due to the possibility that phenotypic and physiological changes in the host might occur along with the uptake of plasmids.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Park J,Park Wdoi
10.1007/s00284-010-9698-ysubject
Has Abstractpub_date
2011-01-01 00:00:00pages
249-54issue
1eissn
0343-8651issn
1432-0991journal_volume
62pub_type
杂志文章abstract：:Ostricacin-1 and ostricacin-2 (Osp-1 and Osp-2) were beta-defensins antimicrobial peptides that were purified from ostrich leukocytes using a cation-exchange column and a semi-prep RP-HPLC column. Both ostricacins were subjected to increased concentrations of monovalent cations (K(+) and Na(+)) and divalent cations (C...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0554-z
更新日期：2007-07-01 00:00:00
abstract：:The duodenal ulcer promoting gene (dupA), located in the plasticity region of Helicobacter pylori (H. pylori), is predicted to form a type IV secretory system (T4SS) with vir genes around dupA. In the study, we investigated the association between the dupA cluster status and the virulence of H. pylori in a littoral re...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0812-z
更新日期：2015-07-01 00:00:00
abstract：:A 4.5-kilobase DNA fragment from Buchnera aphidicola, the endosymbiont of the aphid Schizaphis graminum, was cloned and sequenced. On the basis of homology to Escherichia coli, the following genes were found in the order listed: aroH-thrS-infC-rpmI-rplT. AroH corresponds to the E. coli tryptophan-inhibited 3-deoxy-D-a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00295507
更新日期：1995-05-01 00:00:00
abstract：:A novel Gram-negative gamma-proteobacterium, non-sporulating motile, rod or coccus-shaped bacterium designated as strain PKS7T was isolated from a sediment sample collected from Chilika Lake, Odisha, India and characterized taxonomically using a polyphasic approach. The major quinone was Q8 and major cellular fatty ac...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01802-9
更新日期：2020-01-01 00:00:00
abstract：:Fragments of Synechocystis-DNA driving salt-induced gene expression in E. coli were isolated with translational fusions to a 'lacZ gene. One fragment (fragment 19) showed a NaCl-dependent activation of betaGal expression with the maximum of a ninefold increase in enzyme activity. A similar induction was triggered by t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900347
更新日期：1998-08-01 00:00:00
abstract：:Invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. Candida albicans is the main pathological agent followed by Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis. These pathogens colonize different host tissues in humans as they are able t...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-014-0651-3
更新日期：2014-11-01 00:00:00
abstract：:Sheath rot disease of rice caused by Sarocladium oryzae (Sawada) (=Acrocylindrium oryzae, Sawada) has become an important production constraint in all rice-growing countries. Pathogenicity, phytotoxic metabolites, and random amplified polymorphic DNA (RAPD) markers were used to assess the level of genetic variability ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4509-6
更新日期：2005-06-01 00:00:00
abstract：:Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00294281
更新日期：1995-08-01 00:00:00
abstract：:The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were chara...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006784
更新日期：1999-03-01 00:00:00
abstract：:NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00298381
更新日期：1995-10-01 00:00:00
abstract：:Viral nervous necrosis infections are causing severe problems on aquaculture industry due to ecological and economic impacts. Their causal agent is nervous necrosis virus or nodavirus, which has been classified into four genotypes. Different genotypes correlate with differences in viral pathogenicity. Therefore, ratio...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0880-0
更新日期：2015-11-01 00:00:00
abstract：:A new antagonistic Burkholderia strain, designated MP-1 and producing antifungal activities against various filamentous plant pathogenic fungi, was isolated from the rhizoshere in the Naju area. Cultural characteristic studies strongly suggested that this strain belongs to the genus Burkholderia. The nucleotide sequen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0333-2
更新日期：2006-11-01 00:00:00
abstract：:A Gram positive, thermophilic, and filamentous bacterium, designated strain GIESS004(T), was isolated from a sludge compost from Guangzhou, China. Growth occurred at 45-55 °C (optimum 50-53 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). The organism formed cream-yellow colonies with radial wrinkles. Aerial mycelium was not...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0693-6
更新日期：2015-01-01 00:00:00
abstract：:An aerobic, gram-reaction-negative, rod-shaped bacterium, designated strain Z6(T), was isolated from sediment collected at Mohe Basin, China. And its taxonomic position was investigated by applying a polyphasic approach. Growth occurs at 10-45 °C (optimum, 30 °C), at pH 6.0-11.0 (optimum, pH 7.0) and in the presence o...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1074-0
更新日期：2016-09-01 00:00:00
abstract：:The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9977-2
更新日期：2011-09-01 00:00:00
abstract：:Minimal inhibitory concentrations (MICs) of five antibiotics namely amikacin, gentamicin, tetracycline, ciprofloxacin, and nitrofurantoin for pork isolates of Yersinia enterocolitica increased two- to eightfold after bacteria were grown in the presence of 5 mm arsenite. For Y. enterocolitica isolates obtained from was...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010277
更新日期：2001-08-01 00:00:00
abstract：:We undertook the issue of the distribution of intracellular bacteria among Oribatida (Acari). Six genera of bacteria were detected by PCR and Sanger DNA sequencing: Wolbachia, Cardinium, Rickettsia, Spiroplasma, Arsenophonus, and Hamiltonella. Our research, for the first time, revealed the presence of Cardinium in Mic...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01717-5
更新日期：2019-09-01 00:00:00
abstract：:Cronobacter sakazakii is now recognized as an opportunistic pathogen and has been implicated in rare but severe cases of necrotizing enterocolitis, meningitis, and sepsis in neonates. The first step in bacterial pathogenesis requires that the organism adheres to host cells surfaces; therefore, agents that inhibit adhe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0623-7
更新日期：2014-10-01 00:00:00
abstract：:Bacillus sp. B25 is an effective biocontrol agent against the maize pathogenic fungus Fusarium verticillioides (Fv). Previous in vitro assays have shown that B25 has protease, glucanase, and chitinase activities and siderophores production; however, specific mechanisms by which B25 controls Fv are still unknown. To de...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1372-1
更新日期：2018-03-01 00:00:00
abstract：:High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation ti...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0006-8
更新日期：2002-04-01 00:00:00
abstract：:The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crys...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0085-2
更新日期：2007-09-01 00:00:00
abstract：:The neurotoxin gene of non-proteolytic Clostridium botulinum type B (strain Eklund 17B) was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated with primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 3' end of the gene was obtained by using primer...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01569055
更新日期：1994-02-01 00:00:00
abstract：:Betel vine is an edible creeper used in folk medicine to aid digestion since time immemorial. It is an ideal candidate deemed for the bioprospection of endophytic microorganisms with valuable attributes. This study aimed at the characterization of potential bacteria from fermented betel leaves. We report the presence ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02270-2
更新日期：2021-01-01 00:00:00
abstract：:Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0009-z
更新日期：2011-12-01 00:00:00
abstract：:A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1553-6
更新日期：2018-11-01 00:00:00
abstract：:Thiothrix spp., sulfide-oxidizing filamentous bacteria, were found to be a principal bacterial component of aquatic biofilms causing biofouling in selected municipal water storage tanks, private wells, and drip irrigation systems in Florida. Treatments of up to 200 ppm chlorine in the affected systems could not preven...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900233
更新日期：1997-09-01 00:00:00
abstract：:Helicobacter pylori infection increases the risk of cardiovascular diseases besides leading to duodenal and gastric peptic ulcerations. H. pylori cysteine-rich protein B (HcpB) is a disulfide-rich repeat protein that belongs to the family of Sel1-like repeat proteins. HcpB contains four pairs of anti-parallel alpha he...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9803-2
更新日期：2011-03-01 00:00:00
abstract：:The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0295-z
更新日期：2007-03-01 00:00:00
abstract：:From an undisturbed natural forest soil in Ecuador, three fungal strains of the genus Aspergillus were isolated. Based on molecular and morphological features they are described as three new species, named A. quitensis, A. amazonicus, and A. ecuadorensis. ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9178-9
更新日期：2008-09-01 00:00:00
abstract：:The production and its potential use of a novel trihydroxy unsaturated fatty acid, 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD), were investigated. TOD was formed by Pseudomonas aeruginosa PR3 (NRRL B-18602) in a culture supplied with exogenous ricinoleic acid. The yield of TOD production was always higher in a ric...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010287
更新日期：2001-09-01 00:00:00
