Abstract：
:The neurotoxin gene of non-proteolytic Clostridium botulinum type B (strain Eklund 17B) was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated with primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 3' end of the gene was obtained by using primers designed to the determined sequence of non-proteolytic BoNT/B and a published downstream region of BoNT/B gene from a proteolytic strain. Translation of the nucleotide sequence derived from cloned PCR fragments demonstrated the toxin gene encodes a protein of 1291 amino acid residues. Comparative alignment of the derived BoNT/B sequence with those of other published botulinal neurotoxins revealed highest sequence relatedness with BoNT/B of proteolytic C. botulinum. The sequence identity between non-proteolytic and proteolytic BoNT/B was 97.7% for the light chain (corresponding to 10 amino acid changes) and 90.2% for the heavy chain (corresponding to 81 amino acid changes), with most differences occurring at the C-terminal end. A genealogical tree constructed from all known botulinal neurotoxin sequences revealed marked topological differences with a phylogenetic tree of C. botulinum types based upon small-subunit (16S) ribosomal RNA sequences.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Hutson RA,Collins MD,East AK,Thompson DEdoi
10.1007/BF01569055subject
Has Abstractpub_date
1994-02-01 00:00:00pages
101-10issue
2eissn
0343-8651issn
1432-0991journal_volume
28pub_type
杂志文章abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0095-4
更新日期：2002-08-01 00:00:00
abstract：:To determine the effect of organics (yeast extract) on microbial community during chalcopyrite bioleaching at different temperature, real-time polymerase chain reaction (PCR) was employed to analyze community dynamics of major bacteria applied in bioleaching. The results showed that yeast extract exerted great impact ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9960-y
更新日期：2011-08-01 00:00:00
abstract：:High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation ti...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0006-8
更新日期：2002-04-01 00:00:00
abstract：:Six cyanobacterial isolates recovered from Polynesian microbial mats, called "kopara," were cultured using laboratory-closed photobioreactors and were shown to produce exopolymers as released and capsular exopolysaccharides (EPS). These polymers have been chemically characterized using colorimetric and elemental assay...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0069-z
更新日期：2005-12-01 00:00:00
abstract：:The Gram-stain-negative, rod-shaped, aerobic strain, designated YC1039T, was isolated from a seamount northern Mariana Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YC1039T was related to the genus Mesoflavibacter and had highest 16S rRNA gene sequence...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1500-6
更新日期：2018-09-01 00:00:00
abstract：:Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not pr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3976-2
更新日期：2003-09-01 00:00:00
abstract：:In this study, the effect of rhamnolipid biosurfactant produced by Pseudomonas fluorescens on bacterial strains, laboratory strains, and isolates from industrial wastewater was investigated. It was shown that biosurfactant, depending on the concentration, has a neutral or detrimental effect on the growth and protein r...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9725-z
更新日期：2011-02-01 00:00:00
abstract：:Favre and coworkers (Favre et al., Biochimie 73:235-244, 1991) previously reported that the Bordetella pertussis recA gene present at high copy number could promote a low frequency of recombination, but not bacteriophage induction in Escherichia coli RecA- mutants. Reexamination of these phenotypes demonstrated that, ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900029
更新日期：1996-03-01 00:00:00
abstract：:Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02075-3
更新日期：2020-10-01 00:00:00
abstract：:The natural biodiversity that is found in tropical areas offers countless biotechnological opportunities; especially if we take in account that many biomolecules from several microorganisms have supported for many years, different industrial applications in areas such as pharmacology, agro-industry, bioprocess, enviro...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0023-1
更新日期：2011-12-01 00:00:00
abstract：:To well understand the community structure and composition of mesophilic microorganisms in anaerobic system fed with PTA wastewater, an up-flow anaerobic fixed bed reactor was continuously run at 33 and 37 °C for 75 and 60 days, respectively. Both fluorescence in situ hybridization analysis and 454-pyrosequencing were...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0884-9
更新日期：2015-11-01 00:00:00
abstract：:We have shown previously that Spo0A approximately P-dependent sinIR operon expression was substantially down-regulated in abrB null mutant backgrounds. In this report, we show that loss of function mutations in abrB also cause phosphorelay gene expression to be down regulated. abrB null mutations caused diminished veg...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4186-2
更新日期：2004-04-01 00:00:00
abstract：:Pandemic influenza A (H1N1) 2009 virus was first detected in India in May 2009 which subsequently became endemic in many parts of the country. Influenza A viruses have the ability to evade the immune response through its ability of antigenic variations. The study aims to characterize influenza A (H1N1) pdm 09 viruses ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1262-6
更新日期：2017-08-01 00:00:00
abstract：:The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0243-z
更新日期：2013-01-01 00:00:00
abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3886-3
更新日期：2003-04-01 00:00:00
abstract：:Cosubstrates fermentation is such an effective strategy for increasing subject metabolic products that it could be available and studied in propionic acid production, using glycerol and glucose as carbon resources. The effects of glycerol, glucose, and their mixtures on the propionic acid production by Propionibacteri...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9683-5
更新日期：2011-01-01 00:00:00
abstract：:The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3767-9
更新日期：2002-12-01 00:00:00
abstract：:The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01898-4
更新日期：2020-06-01 00:00:00
abstract：:A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% iden...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4467-z
更新日期：2006-04-01 00:00:00
abstract：:A modification is described of the plate method for the detection of microorganisms with overall cellulolytic activity, including those like Cytophaga, in which the activity is cell bound. Within a few days of incubation colonies of cellulose-degrading bacteria formed holes in discs of lens paper placed on freshly ino...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910027
更新日期：2000-02-01 00:00:00
abstract：:Pseudomonas putida E41 isolated from root interior of Sida hermaphrodita (grown on a field contaminated with heavy metals) showed high biosurfactant activity. In this paper, we describe data from mass spectrometry and genome analysis, to improve our understanding on the phenotypic properties of the strain. Supernatant...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01757-x
更新日期：2019-11-01 00:00:00
abstract：:The invertase of Lactobacillus reuteri CRL 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kDa. The enzyme was stable below 45 degrees C over a wide pH range (4.5-7.0) with maximum activity at pH 6.0 and 37 degrees C. The invertase activity was significantly inhibited by bivalent meta...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910036
更新日期：2000-03-01 00:00:00
abstract：:Probiotics are well known for their wide range of beneficial activities. However, recent use of probiotic Bifidobacterium, Enterococcus, and Lactobacillus spp. has been plagued by certain disadvantages such as complex growth requirements, high maintenance cost, susceptibility to the gastrointestinal environment, patho...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01647-2
更新日期：2019-04-01 00:00:00
abstract：:The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cu...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900258
更新日期：1997-11-01 00:00:00
abstract：:Pantoea beijingensis, a gram-negative pathogenic bacterium, causes soft rot disease in the fungus Pleurotus eryngii in China. However, the taxonomic classification of this pathogen is controversial due to close relationships between bacteria of the genera Pantoea and Erwinia. This study aimed to resolve the identity o...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02253-3
更新日期：2021-01-01 00:00:00
abstract：:A bacterium that tested positive with antibodies specific for Escherichia coli O157 was isolated from beef during routine screening procedures. The bacterium was identified as E. fergusonii by biochemical testing and partial sequencing of 16S rRNA. The isolate was tested for the presence of genes encoding Shiga toxins...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0447-6
更新日期：2006-06-01 00:00:00
abstract：:Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900462
更新日期：1999-11-01 00:00:00
abstract：:Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9602-9
更新日期：2010-10-01 00:00:00
abstract：:The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9107-y
更新日期：2008-05-01 00:00:00
abstract：:Bacterial communities communicate, regulate and coordinate their cooperative activities and physiological process by releasing, sensing and responding to small diffusible signal molecules such as acyl homoserine lactones (AHLs), auto-inducing oligo-peptides (AIPs) and autoinducer-2, a process referred to as Quorum sen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02183-0
更新日期：2020-11-01 00:00:00
