Cloning, sequencing and characterization of a novel phosphatase gene, phoI, from soil bacterium Enterobacter sp. 4.

Abstract:

:A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% identity and 62.4% homology to the hel gene from Heamophilus influenzae, which encoded an outer membrane protein (P4). The optimum pH and temperature for phosphatase activity were pH 5.5 and 40 degrees C, respectively. Its specific activity on rho-nitrophenyl phosphatate was 70 U/mg at pH 5.5 and 40 degrees C. Enzyme activity was inhibited by Al3+, EDTA, and DTT, but fivefold activated by Cu2+ ion (350 U/mg). PhoI showed a strong synergistic effect when used with a purified E. coli phytase, AppA, to estimate combination effects.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Kang SH,Cho KK,Bok JD,Kim SC,Cho JS,Lee PC,Kang SK,Lee HG,Woo JH,Lee HJ,Lee SC,Choi YJ

doi

10.1007/s00284-005-4467-z

keywords:

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

243-8

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

52

pub_type

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