Abstract:
:Pseudomonas putida E41 isolated from root interior of Sida hermaphrodita (grown on a field contaminated with heavy metals) showed high biosurfactant activity. In this paper, we describe data from mass spectrometry and genome analysis, to improve our understanding on the phenotypic properties of the strain. Supernatant derived from P. putida E41 liquid culture exhibited a strong decrease in the surface tension accompanied by the ability for emulsion stabilization. We identified extracellular lipopeptides, putisolvin I and II expression but did not detect rhamnolipids. Their presence was confirmed by matrix-assisted laser desorption and ionization (MALDI) TOF/TOF technique. Moreover, ten phospholipids (mainly phosphatidylethanolamines PE 33:1 and PE 32:1) which were excreted by vesicles were also detected. In contrast the bacterial cell pellet was dominated by phosphatidylglycerols (PGs), which were almost absent in the supernatant. It seems that the composition of extracellular (secreted to the environment) and cellular lipids in this strain differs. Long-read sequencing and complete genome reconstruction allowed the identification of a complete putisolvin biosynthesis pathway. In the genome of P. putida E41 were also found all genes involved in glycerophospholipid biosynthesis, and they are likely responsible for the production of detected phospholipids. Overall this is the first report describing the expression of extracellular lipopeptides (identified as putisolvins) and phospholipids by a P. putida strain, which might be explained by the need to adapt to the highly contaminated environment.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Bernat P,Nesme J,Paraszkiewicz K,Schloter M,Płaza Gdoi
10.1007/s00284-019-01757-xsubject
Has Abstractpub_date
2019-11-01 00:00:00pages
1320-1329issue
11eissn
0343-8651issn
1432-0991pii
10.1007/s00284-019-01757-xjournal_volume
76pub_type
杂志文章abstract::A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010186
更新日期:2001-02-01 00:00:00
abstract::Pneumatophores are aerial roots developing from the main roots of mangrove plants away from the gravity. The below ground pneumatophore-associated soil prokaryotic community of Avicennia marina was studied by amplicon pyrosequencing (39,378 reads) during monsoon and summer seasons. Apart from the most dominant phylum ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0920-9
更新日期:2016-01-01 00:00:00
abstract::An in vitro study was conducted to examine the metabolism of histidine (His) by mixed rumen bacteria (B), mixed rumen protozoa (P), and a combination of the two (BP). Rumen microorganisms were collected from fistulated goats fed with lucerne cubes (Medicago sativa) and a concentrate mixture twice a day. Microbial susp...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010170
更新日期:2001-01-01 00:00:00
abstract::Invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. Candida albicans is the main pathological agent followed by Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis. These pathogens colonize different host tissues in humans as they are able t...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-014-0651-3
更新日期:2014-11-01 00:00:00
abstract::The diversities of soil bacterial communities in the Phragmites australis, Phalaris arundinacea, and non-plantation constructed wetlands were compared and analyzed by through high-throughput Illumina sequencing. At the phylum level, the ten dominant bacterial phyla in the three types of constructed wetlands were the s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01634-7
更新日期:2019-03-01 00:00:00
abstract::Protease production by Streptomyces sp. 594 was obtained after submerged fermentation (SF) and solid-state fermentation (SSF) using feather meal (FM) and corn steep liquor (CSL) as sole sources of carbon and nitrogen. Enzyme productions were 13.4 U ml(-1) in SF and 21.5 U g(-1) in SSF; these values were approximately ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0163-x
更新日期:2006-10-01 00:00:00
abstract::Potassium ion pool was studied in glycolyzing Enterococcus hirae, grown at high or low alkaline pH (pH 9.5 and 8.0, respectively). Energy-dependent increase of K+ pool was lower for the wild-type cells, grown at pH 9.5, than that for the cells grown at pH 8.0. It was inhibited by N,N'-dicyclohexylcarbodiimide (DCCD). ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900289
更新日期:1998-02-01 00:00:00
abstract::The citric acid-producing fungus Aspergillus niger WU-2223L possesses a cyanide-insensitive respiratory pathway catalyzed by alternative oxidase. The regulation of the alternative oxidase under the conditions of citric acid production was determined from the transcription level of the alternative oxidase gene (aox1). ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9369-z
更新日期:2009-04-01 00:00:00
abstract::The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in th...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010199
更新日期:2001-03-01 00:00:00
abstract::Salinity, a serious environmental pressure on crop production, might be counteracted by free-living and symbiotic inoculants entailing positive synergistic effects. Enhancement in nutrient uptake and/or production of antioxidants under the stress condition, can improve plant growth and yield. In this study, inoculatio...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02034-y
更新日期:2020-09-01 00:00:00
abstract::In November 2007, 450 m(3) of treated wastewater leaked into the drinking water distribution system contaminating the drinking water of over 10,000 inhabitants of Nokia, Southern Finland. Nearly 1,000 people visited the health centre because of gastroenteritis during the following 5 weeks. A wide range of enteric path...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9832-x
更新日期:2011-04-01 00:00:00
abstract::Immobilized cells of Enterobacter agglomerans, able to reduce azo dyes enzymatically, were used as a biocatalyst for the decolorization of synthetic medium containing the toxic azo dye methyl red (MR). This bacterial strain exhibits high ability to completely decolorize 100 mg/L of MR after only 6 h of incubation unde...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4143-0
更新日期:2004-02-01 00:00:00
abstract::The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01898-4
更新日期:2020-06-01 00:00:00
abstract::The establishment of human flora-associated animal models allows the in vivo manipulation of host, microbial, and environmental parameters to influence the gut microbial community. However, it is difficult to simulate infant gut microbiota in germ-free animals because of the variation and dynamic state of infant micro...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0360-3
更新日期:2013-09-01 00:00:00
abstract::Cosubstrates fermentation is such an effective strategy for increasing subject metabolic products that it could be available and studied in propionic acid production, using glycerol and glucose as carbon resources. The effects of glycerol, glucose, and their mixtures on the propionic acid production by Propionibacteri...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9683-5
更新日期:2011-01-01 00:00:00
abstract::Lake Elmenteita is one of the alkaline saline lakes within the Kenyan Rift valley. The lake is situated on the floor of the Kenyan Rift Valley at 1,776 m above sea level and has no direct outlet. The microbial diversity of the lake was investigated using a culture-independent approach. Five different sampling points w...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9692-4
更新日期:2011-01-01 00:00:00
abstract::Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14-60 days a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0237-x
更新日期:2013-02-01 00:00:00
abstract::Growth on aniline by three purple non-sulfur bacteria (Rhodospirillum rubrum ATCC 11170, Rhodobacter sphaeroides DSM 158, and Rubrivivax benzoatiliticus JA2) as nitrogen, or carbon source could not be demonstrated. However in its presence, production of indole derivatives was observed with all the strains tested. At l...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9609-2
更新日期:2010-10-01 00:00:00
abstract::Acinetobacter baumannii is an emerging opportunistic pathogen that primarily infects critically ill patients in nosocomial settings and there is a need for identifying new alternative therapeutic agents against these organisms. Ceragenins are non-peptide, membrane-active agents that mimic the antimicrobial properties ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01949-w
更新日期:2020-08-01 00:00:00
abstract::Endophytes are beneficial microbes that reside intercellularly inside the plants. Interaction of endophytes with the host plants and their function within their host are important to address ecological relevance of endophyte. Four endophytic bacteria OS-9, OS-10, OS-11, and OS-12 were isolated from healthy leaves of O...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9520-x
更新日期:2010-03-01 00:00:00
abstract::The production of linolenic acid in mycelial lipids reached 0.31 mg/ml of culture broth when Mortierella isabellina was cultivated in a medium consisting of 2% octadecanol, 1% yeast extract, and 25 mmol/L of Mg2+ at 23 degrees C for 5 days. Cultivation conditions were studied, and the results showed that (i) a suitabl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0031-7
更新日期:2002-02-01 00:00:00
abstract::Enterotoxigenic Escherichia coli causes diarrhea by producing several virulence factors including heat-labile enterotoxin (LT). LT is maximally expressed at 37 degrees C. The histone-like nucleoid structuring protein (H-NS) appears to inhibit LT expression by binding to a downstream regulatory element (DRE) at low tem...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4282-y
更新日期:2004-11-01 00:00:00
abstract::A rhizobacterium with high antifungal activity was isolated from a potato field at Inneruulalik, South Greenland. Phylogenetic analysis based on multi locus sequence typing showed that the bacterium was affiliated with strains of Pseudomonas fluorescens. The bacterium, denoted as Pseudomonas fluorescens In5, inhibited...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9846-4
更新日期:2011-04-01 00:00:00
abstract::A total of 168 Campylobacter strains (154 C. jejuni and 14 C. coli) isolated from human clinical samples and chicken meat were typed using Penner serotyping, randomly amplified polymorphic DNA (RAPD), and pulsed-field gel electrophoresis (PFGE) with four restriction enzymes (Sac II, Sal I, Sma I, Kpn I). The 168 strai...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-4037-6
更新日期:2003-11-01 00:00:00
abstract::The cyclomaltodextrinase gene from Bacillus subtilis high-temperature growth transformant H-17 was cloned on separate PstI, BamHI, and EcoRI fragments into the plasmid vector pUC18, but was expressed in an inactive form in the host, Escherichia coli DH5 alpha. High level constitutive expression of the gene product was...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01577379
更新日期:1993-04-01 00:00:00
abstract::Bacteria were isolated from rabbit faeces using equine caecal fluid as a growth medium. Two new isolates of the genus Streptococcus are described in terms of their biochemical properties. One of these has a 16S rRNA gene with 97.7%, and the other 98.5%, identity to Streptococcus thoraltensis. While S. thoraltensis has...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9619-0
更新日期:2010-10-01 00:00:00
abstract::The adhesive property to the intestinal mucin of Bifidobacterium lactis LKM512, B. longum, B. breve, B. bifidum, B. adolescentis, B. infantis, Bacteroides vulgatus, Bacteroides distasonis, Eubacterium aerofaciens, Clostridium perfringens, Escherichia coli, and Lactobacillus acidophilus were examined. Adhesive rate of ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0087-4
更新日期:2002-03-01 00:00:00
abstract::Bacillus sp. TYF-LIM-B05, which is isolated from spoilage vinegar, is resistant to high temperature, high concentrated alcohol, acid, and salt, and can produce ethanol from mono-, di-, polysaccharide, and complex biomass as the sole carbon source. Thus, this strain is a potential candidate for consolidated bioprocessi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01833-2
更新日期:2020-03-01 00:00:00
abstract::Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultur...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1284-0
更新日期:2017-09-01 00:00:00
abstract::Nine structurally similar macrolide antibiotics were tested at a concentration of 0.5 microg/ml for their relative inhibitory effects on ribosome functions in Staphylococcus aureus cells. Eight of the compounds examined inhibited protein synthesis at this concentration. Seven of the nine compounds were also effective ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900402
更新日期:1998-12-01 00:00:00