Abstract：
:The cyclomaltodextrinase gene from Bacillus subtilis high-temperature growth transformant H-17 was cloned on separate PstI, BamHI, and EcoRI fragments into the plasmid vector pUC18, but was expressed in an inactive form in the host, Escherichia coli DH5 alpha. High level constitutive expression of the gene product was also detrimental to the E. coli host, which led to structural instability of the recombinant plasmid. The cyclomaltodextrinase gene was cloned on a 3-kb EcoRI fragment into the plasmid vector pPL708, and the fragment was structurally maintained in the host B. subtilis YB886. The cloned gene product was synthesized in an enzymatically active form in the B. subtilis host; however, expression was at a low level. Subcloning of the 3-kb EcoRI fragment into pUC18 and transformation into E. coli XL1-Blue (F' lacIq) indicated that the cyclomaltodextrinase gene was cloned with its own promoter, since expression of the gene occurred in the absence of IPTG. Subcloning of the cyclomaltodextrinase gene downstream from the Bacillus temperature phage SPO2 promoter of pPL708 may increase expression of this gene.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Krohn BM,Lindsay JAdoi
10.1007/BF01577379subject
Has Abstractpub_date
1993-04-01 00:00:00pages
217-22issue
4eissn
0343-8651issn
1432-0991journal_volume
26pub_type
杂志文章abstract：:A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010186
更新日期：2001-02-01 00:00:00
abstract：:Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9347-x
更新日期：2009-04-01 00:00:00
abstract：:Low-substrate regulated microaerophilic behavior (LSRMB) was observed in 10-54% of the bacteria isolated from several fresh-water lakes or ponds, subsurface soils, activated sludge, and Antarctic dry valley soils. Five Pseudomonas and two Bacillus type species showed LSRMB. A subsurface Pseudomonas jessenii strain was...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010097
更新日期：2000-08-01 00:00:00
abstract：:Lipids contribute significantly to the pathogenesis of fungal infectious diseases and an understanding of lipid metabolism occurring in fungal pathogens can help the development of more efficient antifungal therapeutic strategies. In this study, the effect of culture age on the distribution of fatty acids among differ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9052-1
更新日期：2008-02-01 00:00:00
abstract：:Bt cotton was the first genetically modified crop approved for use in India. However, only a few studies have been conducted to assess the feasibility of its commercial application. Bt cotton is genetically modified to express a proteinaceous endotoxin (Cry) encoded by cry gene of Bacillus thuringiensis that has speci...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9585-6
更新日期：2010-08-01 00:00:00
abstract：:Legionella and amebae populations in 16 cooling towers were challenged with three commercially available biocide formulations. The active agents were: a chlorinated phenolic thioether (CPTE), bromo-nitro-propane-diol (BNPD), and bromo-chloro-dimethylhydantoin (BCD, in briquette form). The towers were dosed with these ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00296203
更新日期：1995-03-01 00:00:00
abstract：:Pseudomonas fluorescens is a physiologically diverse species of bacteria present in many habitats, which possesses multifunctional traits that provide it with the capability to exhibit biological control activities, promote plant health or cause plant disease. Here, we present the draft genome sequence of the kiwifrui...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01655-2
更新日期：2019-05-01 00:00:00
abstract：:We report on the first characterization of the culturable heterotrophic bacteria of the scleractinian Madracis decactis. In addition, we characterized the culturable bacteria associated with the fireworm Hermodice carunculata, observed predating partially bleached coral colonies. Our study was carried out in the remot...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0435-1
更新日期：2014-01-01 00:00:00
abstract：:Sheath rot disease of rice caused by Sarocladium oryzae (Sawada) (=Acrocylindrium oryzae, Sawada) has become an important production constraint in all rice-growing countries. Pathogenicity, phytotoxic metabolites, and random amplified polymorphic DNA (RAPD) markers were used to assess the level of genetic variability ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4509-6
更新日期：2005-06-01 00:00:00
abstract：:Although extensive information is available on the effect ultraviolet (UV) radiation has on Gram-negative marine bacteria, there is a scarcity of data concerning UV radiation and Gram-positive marine bacteria. The focus of this paper is on Microbacterium maritypicum, with the Gram-negative Vibrio natriegens being used...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0349-2
更新日期：2007-07-01 00:00:00
abstract：:A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02210-0
更新日期：2020-11-01 00:00:00
abstract：:We report the in vitro phytopathogen suppression activity of siderophoregenic preparations of Alcaligenes feacalis vis-à-vis the oraganochlorine fungicide, bavistin. Siderophore-rich culture broth, siderophore-rich supernatant, and purified siderophore preparation exerted antifungal activity against Aspergillus niger ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9264-z
更新日期：2009-01-01 00:00:00
abstract：:We studied the usefulness of flow cytometry for detection of penicillin resistance in E. faecalis and S. aureus by direct binding of commercially available fluorescent penicillin, Bocillin FL, to cells obtained from culture. There were significantly lower percentages of fluorescent cells and median and mean fluorescen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9179-8
更新日期：2008-08-01 00:00:00
abstract：:A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% iden...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4467-z
更新日期：2006-04-01 00:00:00
abstract：:To date, a few works have performed a correlation of metabolic variables in bacteria; however specific correlations with these variables have not been reported. In this work, we included 36 human pathogenic bacteria and 18 non- or less-pathogenic-related bacteria and obtained all metabolic variables, including enzymes...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1013-0
更新日期：2016-06-01 00:00:00
abstract：:Campylobacter coli cells are characterized by a comma, or spiral shape, and a single polar flagellum. Here we report stable spontaneous changes in morphology to an aflagellated straight rod. The ability to colonize the chick cecum was lost. The atypical cells were compared with the original C. coli 67 culture from whi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4527-4
更新日期：2005-09-01 00:00:00
abstract：:NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00298381
更新日期：1995-10-01 00:00:00
abstract：:Lactobacilli are members of the normal mucosal microflora of most animals. Many isolates of Lactobacillus spp. are adherent to epithelial cells. In this study, using Lactobacillus acidophilus and L. agilis, we detected adherence in a pattern that suggested that the bacteria were binding to extracellular matrix protein...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010107
更新日期：2000-08-01 00:00:00
abstract：:His(354) and His(358), two highly conserved histidines in Xenopus laevis (6-4) photolyase [equivalent to His(401) and His(405), in Dunaliella salina (6-4) photolyase], are critical for photoreactivation. They act as a base and an acid, respectively. However, the remaining high repair activity when the pH value is high...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9687-1
更新日期：2011-01-01 00:00:00
abstract：:The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0295-z
更新日期：2007-03-01 00:00:00
abstract：:In Neurospora crassa, multinucleate macroconidia are used for genetic transformation. The barrier for such a transformation can be either at the cell membrane level or at the nuclear membrane level. For assessment of these possibilities, a forced heterokaryon (containing two genetically marked nuclei and auxotrophic f...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0004-x
更新日期：2002-05-01 00:00:00
abstract：:PHA is one of the leading commercially important bio-polyesteric compounds piled up as an intracellular lipid-based energy storage compound by numerous microorganisms. An indigenous Gram-positive bacterium isolated from fire ant (Solenopsis invicta) has known to potentially accumulate PHA. Various nutritional elements...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02221-x
更新日期：2020-12-01 00:00:00
abstract：:The production and its potential use of a novel trihydroxy unsaturated fatty acid, 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD), were investigated. TOD was formed by Pseudomonas aeruginosa PR3 (NRRL B-18602) in a culture supplied with exogenous ricinoleic acid. The yield of TOD production was always higher in a ric...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010287
更新日期：2001-09-01 00:00:00
abstract：:Production of carbon dioxide, as one of the ultimate products of fungal metabolism, can be used to quantify and measure their metabolic rate under different conditions, thus aiding in finding the optimal substrate and environment for cultivation of wood-destroying fungi. This study is focused on species Pleurotus ostr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02033-z
更新日期：2020-09-01 00:00:00
abstract：:The yeast Candida glycerinogenes produces a high yield of glycerol only in response to a medium-osmotic stress, but little is known about the relationship between osmoadaptation and glycerol metabolism. The CgTPI gene encoding triose phosphate isomerase of C. glycerinogenes was cloned and sequenced, and its functional...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0070-9
更新日期：2007-08-01 00:00:00
abstract：:Next Generation Sequencing (NGS), using the Illumina® metabarcoding system, showed differences between biofilm communities on three degraded siliceous stone church façades in central Rio de Janeiro. Two church biofilms (on granite and augen gneiss) were dominated by Actinobacteria; the third (granite), surrounded by t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1257-3
更新日期：2017-07-01 00:00:00
abstract：:To investigate the correlation between mutations in promoter, attenuator, and the AmpC enzyme overproduction in Escherichia coli. ampC Promoters from 4 Escherichia coli clinical isolates were cloned upstream to the chloramphenicol acetyltransferase (CAT) gene in pCAT3 reporter plasmid. Promoter strengths were measured...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9426-7
更新日期：2009-09-01 00:00:00
abstract：:Stenotrophomonas maltophilia is an emerging opportunistic pathogen linked not only to bacteremia, sepsis, and pneumonia but also to severe chronic enteritis. Persons with the impaired immune system are prone to be infected by S. maltophilia since its pathogenicity seems to be more associated with the host immune syste...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1559-0
更新日期：2018-12-01 00:00:00
abstract：:Methanogenesis from furfural by defined mixed cultures was studied. Under sulfate-reducing conditions, a Desulfovibrio strain was used as the furfural-degrading species producing acetic acid. This sulfate-reducing bacterium (SRB) Desulfovibrio strain B is an incomplete oxidizer, unable to carry out the terminal oxidat...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0010-z
更新日期：2002-06-01 00:00:00
abstract：:This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9038-z
更新日期：2008-01-01 00:00:00
