Abstract：
:In Neurospora crassa, multinucleate macroconidia are used for genetic transformation. The barrier for such a transformation can be either at the cell membrane level or at the nuclear membrane level. For assessment of these possibilities, a forced heterokaryon (containing two genetically marked nuclei and auxotrophic for histidine) of Neurospora crassa was transformed with a plasmid containing his-3+ gene. The transformants, which could grow without histidine supplementation, were then resolved into component homokaryons to determine into which nucleus or nuclei the plasmid had entered. Our results suggest that the barrier for transformation in Neurospora crassa is at the nuclear level, not at the cell membrane level. In a heterokaryon containing two genetically distinct nuclei, plasmid DNA integrated into only one of the nuclear types at any instance, but never into both nuclear types. Thus, in Neurospora crassa, the competent nucleus is essential for the transformation event to take place, and at a given time only one type of nucleus is competent to take up the exogenous DNA. Genomic Southern analysis showed that the transformants harbor both ectopic and homologous integrations of the plasmid DNA. The type and number of integrations were reflected at the post-translational level, since the specific activity of histidinol dehydrogenase (the translation product of his-3+ gene) was variable among several transformants and always less than the level of the wild type.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Dev K,Maheshwari Rdoi
10.1007/s00284-001-0004-xkeywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
309-13issue
5eissn
0343-8651issn
1432-0991journal_volume
44pub_type
杂志文章abstract：:Physiological alterations and regulation of heterocyst and nitrogenase formation have been studied in Het(-) Fix(-) mutant strain of diazotrophic cyanobacterium Anabaena variabilis. Het(-) Fix(-) mutant strain of A. variabilis has been isolated by N-methyl-N'-nitro-N"-nitrosoguanidine (NTG) mutagenesis and was screene...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3756-z
更新日期：2002-11-01 00:00:00
abstract：:During periods of drainage, both water stress and oxygen can cause damage to indigenous methanogens. In the present study, we evaluated the tolerance of seven methanogenic strains (Methanobrevibacter arboriphilicus, Methanobacterium formicicum, Methanococcus vannielii, Methanospirillum hungatei, Methanoculleus olentan...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9059-7
更新日期：2008-03-01 00:00:00
abstract：:Root colonization and diversity of arbuscular mycorrhizal fungi (AMF) were analyzed in plants growing in fly ash pond. Eight species could be separated morphologically, while phylogenetic analyses after PCR amplification of the ITS region followed by RFLP and sequencing revealed seven different AM fungal sequence type...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9974-5
更新日期：2011-09-01 00:00:00
abstract：:The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9643-0
更新日期：2010-12-01 00:00:00
abstract：:With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900272
更新日期：1998-01-01 00:00:00
abstract：:This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9038-z
更新日期：2008-01-01 00:00:00
abstract：:Marine subsurface sediments represent a novel archaeal biosphere with unknown physiology. To get to know the composition and ecological roles of the archaeal communities within the sediments of the Pearl River Estuary, Southern China, the diversity and vertical distribution of active archaea in a sediment core were ch...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0320-y
更新日期：2013-07-01 00:00:00
abstract：:Broad variation among anoxygenic reaction centers makes it essential to consider a wide variety when considering the origins of photosynthesis. The photosynthetic core antenna domain in the gene pshA from Heliophilum fasciatum was sequenced doubling the number of core sequences available from heliobacteria. The sequen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4221-3
更新日期：2004-06-01 00:00:00
abstract：:Genetic engineering of Lactococcus lactis to produce a heterologous protein may cause potential risks to the environment despite the industrial usefulness of engineered strains. To reduce the risks, we generated three auxotrophic recombinant L. lactis subsp. lactis IL1403 strains expressing a heterologous protein, Bmp...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9572-y
更新日期：2010-07-01 00:00:00
abstract：:The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and Sinorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacte...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010222
更新日期：2001-05-01 00:00:00
abstract：:Lactic-acid-producing bacteria (LABs) are known to have immunomodulating activity. In the current study, various LABs were tested for their immunity-enhancing activity, especially the phagocytic activity of leukocytes. Viable but not heat-killed cells of Weissella kimchii strain PL9001, Lactobacillus fermentum strain ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4377-5
更新日期：2005-01-01 00:00:00
abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0095-4
更新日期：2002-08-01 00:00:00
abstract：:Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and ex...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0114-5
更新日期：2002-09-01 00:00:00
abstract：:Invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. Candida albicans is the main pathological agent followed by Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis. These pathogens colonize different host tissues in humans as they are able t...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-014-0651-3
更新日期：2014-11-01 00:00:00
abstract：:A single strain, designated BF49(T), was isolated from a biofilm of a tufa deposit from the Westerhöfer rivulet, Lower Saxony, Germany. The G+C content of the genomic DNA of strain BF49(T) was 69 mol% and the predominant ubiquinone was Q-8. Major fatty acids were C(16:1)omega7c/15 iso 2OH and C(16:0). Comparative 16S ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9095-3
更新日期：2008-04-01 00:00:00
abstract：:A high-virulence clone (HVC) was proposed as causing much of the morbidity and mortality when a collection of group B Streptococcus (GBS) isolates was examined by multi-locus enzyme electrophoresis. HVC isolates could be further distinguished by their inability to grow at 40 degrees C, and a temperature-sensitive aldo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3957-5
更新日期：2003-10-01 00:00:00
abstract：:A novel actinomycete, designated strain LAM7112T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The new isolate was found to be able to grow at 20-45 °C (optimum: 35 °C), pH 5.0-10.0 (optimum: 7.0) and in the presence of 0-10.0% (optimum: 3.0%) (w/v) NaCl. The ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02192-z
更新日期：2020-11-01 00:00:00
abstract：:The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0021-9
更新日期：2002-05-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake in the provided KEMB accession number. ...
journal_title：Current microbiology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00284-018-1592-z
更新日期：2019-01-01 00:00:00
abstract：:An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02130-z
更新日期：2020-10-01 00:00:00
abstract：:A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4-37 °C (optimum: 30 °C) and pH 4-9 (optimum: pH 6), as well as in the presence of 0-6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-sh...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-020-02296-6
更新日期：2021-01-02 00:00:00
abstract：:In the present work, Aspergillus fumigatus is described as a higher producer of hydrolytic enzymes secreted in response to the presence of the Callosobruchus maculatus bruchid pest. This fungus was able to grow over cowpea weevil shells as a unique carbon source, secreting alkaline proteolytic and chitinolytic enzymes...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0192-x
更新日期：2006-06-01 00:00:00
abstract：:Serratia marcescens is an opportunistic pathogen responsible for causing nosocomial infections, corneal ulcer, necrotizing fasciitis, cellulites, and brain abscess. Alkaline phosphatase (APase) is believed to play an important role in the survival of several intracellular pathogens and their adaptation. We have studie...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3740-7
更新日期：2002-10-01 00:00:00
abstract：:The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were chara...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006784
更新日期：1999-03-01 00:00:00
abstract：:Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) an...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01922-7
更新日期：2020-07-01 00:00:00
abstract：:Nine structurally similar macrolide antibiotics were tested at a concentration of 0.5 microg/ml for their relative inhibitory effects on ribosome functions in Staphylococcus aureus cells. Eight of the compounds examined inhibited protein synthesis at this concentration. Seven of the nine compounds were also effective ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900402
更新日期：1998-12-01 00:00:00
abstract：:An isolate of Lactococcus lactis from fermented milk was found to produce a bacteriocin peptide. The isolate could grow in a medium with an initial pH of 11.0, in which it produced the bacteriocin extracellularly at the highest level. The level of the bacteriocin in the medium increased in parallel to the bacterial gr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4545-2
更新日期：2005-09-01 00:00:00
abstract：:Spontaneous white mutants from sectors of Streptomyces hygroscopicus 111-81 were isolated. The comparison of morphological, cultural, and biochemical properties of the mutants and ancestor showed the differences in colors of aerial, substrate mycelia, and sporulation. Changes in resistance to antibiotics and sensitivi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9165-1
更新日期：2008-08-01 00:00:00
abstract：:A Gram positive, thermophilic, and filamentous bacterium, designated strain GIESS004(T), was isolated from a sludge compost from Guangzhou, China. Growth occurred at 45-55 °C (optimum 50-53 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). The organism formed cream-yellow colonies with radial wrinkles. Aerial mycelium was not...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0693-6
更新日期：2015-01-01 00:00:00
abstract：:Subsurface Wastewater Infiltration System (SWIS) is a sewage ecological treatment technology with low investment, energy consumption, and operating cost. SWIS soil contains a large variety of microorganisms. The metabolic process and production of microorganisms are an important basis for qualitatively describing the ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01830-5
更新日期：2020-02-01 00:00:00
