Abstract:
:The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and Sinorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacteroids. The presence of free PQQ in alfalfa root exudates has also been assayed. It was shown that apo-GDH or holoenzyme was actively synthesized by these rhizobia, with the concomitant production of gluconate from glucose, under certain environmental conditions. GDH activity was also detected in bacteroids from alfalfa root nodules inoculated with either S. meliloti RCR2011 or 102F34. It was also shown that free PQQ was present in root exudates of alfalfa, but its production is ascribed to the activity of Erwinia sp., a normal contaminant of these seeds.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Bernardelli CE,Luna MF,Galar ML,Boiardi JLdoi
10.1007/s002840010222keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
310-5issue
5eissn
0343-8651issn
1432-0991journal_volume
42pub_type
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