Periplasmic PQQ-dependent glucose oxidation in free-living and symbiotic rhizobia.

Abstract:

:The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and Sinorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacteroids. The presence of free PQQ in alfalfa root exudates has also been assayed. It was shown that apo-GDH or holoenzyme was actively synthesized by these rhizobia, with the concomitant production of gluconate from glucose, under certain environmental conditions. GDH activity was also detected in bacteroids from alfalfa root nodules inoculated with either S. meliloti RCR2011 or 102F34. It was also shown that free PQQ was present in root exudates of alfalfa, but its production is ascribed to the activity of Erwinia sp., a normal contaminant of these seeds.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Bernardelli CE,Luna MF,Galar ML,Boiardi JL

doi

10.1007/s002840010222

keywords:

subject

Has Abstract

pub_date

2001-05-01 00:00:00

pages

310-5

issue

5

eissn

0343-8651

issn

1432-0991

journal_volume

42

pub_type

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