Abstract：
:Polyclonal antibodies against Escherichia coli and fluorescent, secondary, antibodies were immobilized on borosilicate glass fibers pre-treated with 3-glycidyloxypropyl trimethoxysilane (GPS). Light with an average wavelength of 627 nm, emitted by a diode placed at one end of the glass fiber, was detected by an ultrasensitive photodiode with peak sensitivity at 640 nm. Changes in fluorescence, caused by binding of E. coli to the antibodies, changed the net refractive index of the glass fiber and thus the internal reflection of light. These evanescent changes in photon energy were recorded by an ultrasensitive photodiode. Signals were amplified and changes in voltage recorded with a digital multimeter. A linear increase in voltage readings was recorded over 2 h when 3.0 × 107 CFU/ml and 2.77 × 109 CFU/ml E. coli were adhered to the antibodies. Voltage readings were recorded with E. coli cell numbers from 2 × 103 CFU/ml to 2 × 106 CFU/ml, but readings remained unchanged for 2 h, indicating that the limit of detection is 3.0 × 107 CFU/ml. This simple technology may be used to develop a low-cost, portable, fiber-optic biosensor to detect E. coli in infections and may have applications in the medical field. Research is in progress to optimize the sensitivity of the fiber-optic biosensor and determine its specificity.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Maas MB,Maybery GHC,Perold WJ,Neveling DP,Dicks LMTdoi
10.1007/s00284-017-1359-ysubject
Has Abstractpub_date
2018-02-01 00:00:00pages
150-155issue
2eissn
0343-8651issn
1432-0991pii
10.1007/s00284-017-1359-yjournal_volume
75pub_type
杂志文章abstract：:Microbial populations in indoor environments, where we live and eat, are important for public health. Various bacterial species reside in the kitchen, and refrigerators, the major means of food storage within kitchens, can be a direct source of food borne illness. Therefore, the monitoring of microbiota in the refrige...
journal_title：Current microbiology
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doi：10.1007/s00284-013-0401-y
更新日期：2013-11-01 00:00:00
abstract：:The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...
journal_title：Current microbiology
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更新日期：2010-12-01 00:00:00
abstract：:The ability of Burkholderia sp. VUN10013 to degrade anthracene in microcosms of two acidic Thai soils was studied. The addition of Burkholderia sp. VUN10013 (initial concentration of 10(5) cells g(-1) dry soil) to autoclaved soil collected from the Plew District, Chanthaburi Province, Thailand, supplemented with anthr...
journal_title：Current microbiology
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更新日期：2008-08-01 00:00:00
abstract：:Corynebacterial isolates, obtained by selective and non-selective media from conjunctival swabs, were speciated by using two recently developed systems. All 25 strains from 92 individuals, as well as 12 strains from a previous similar study, belonged to lipophilic species. The significance of this unusual finding is d...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010232
更新日期：2001-05-01 00:00:00
abstract：:Reindeer (Rangifer tarandus tarandus) may include large proportions of lichens in their winter diet. These dietary lichens are rich in phenolic secondary compounds, the most well-known being the antimicrobial usnic acid. Previous studies have shown that reindeer host rumen bacteria resistant to usnic acid and that usn...
journal_title：Current microbiology
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doi：10.1007/s00284-014-0534-7
更新日期：2014-06-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1094-9
更新日期：2016-10-01 00:00:00
abstract：:The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...
journal_title：Current microbiology
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doi：10.1007/s00284-001-0021-9
更新日期：2002-05-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-006-0600-x
更新日期：2007-08-01 00:00:00
abstract：:Several rhizobial strains possess the ability to modulate leguminous plants ethylene levels by producing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase. While the effect of ACC deaminase has been studied in several rhizobia belonging to the Alphaproteobacteria class, not much is understood about its impa...
journal_title：Current microbiology
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doi：10.1007/s00284-018-1474-4
更新日期：2018-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9937-x
更新日期：2011-07-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-009-9375-1
更新日期：2009-06-01 00:00:00
abstract：:Favre and coworkers (Favre et al., Biochimie 73:235-244, 1991) previously reported that the Bordetella pertussis recA gene present at high copy number could promote a low frequency of recombination, but not bacteriophage induction in Escherichia coli RecA- mutants. Reexamination of these phenotypes demonstrated that, ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900029
更新日期：1996-03-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-002-3886-3
更新日期：2003-04-01 00:00:00
abstract：:Pandemic influenza A (H1N1) 2009 virus was first detected in India in May 2009 which subsequently became endemic in many parts of the country. Influenza A viruses have the ability to evade the immune response through its ability of antigenic variations. The study aims to characterize influenza A (H1N1) pdm 09 viruses ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1262-6
更新日期：2017-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900299
更新日期：1998-04-01 00:00:00
abstract：:The Gram-stain-negative, rod-shaped, aerobic strain, designated YC1039T, was isolated from a seamount northern Mariana Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YC1039T was related to the genus Mesoflavibacter and had highest 16S rRNA gene sequence...
journal_title：Current microbiology
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doi：10.1007/s00284-018-1500-6
更新日期：2018-09-01 00:00:00
abstract：:In eukaryotes, loss of cytoplasmic ribosomal proteins (RPs) results in a reduced growth rate and other phenotypic defects. The ability to transition from a unicellular budding yeast to a filamentous form is very important for biofilm formation and virulence in Candida albicans. Our recent study found that loss of the ...
journal_title：Current microbiology
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doi：10.1007/s00284-016-1019-7
更新日期：2016-06-01 00:00:00
abstract：:Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D, and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were r...
journal_title：Current microbiology
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doi：10.1007/s002849900438
更新日期：1999-09-01 00:00:00
abstract：:This study investigated the antifungal effects of cement paste containing Paenibacillus polymyxa E681 against Aspergillus niger, a deleterious fungus commonly found in cement buildings and structures. To test the antifungal effects, cement paste containing P. polymyxa E681 was neutralized by CO2 gas, and the fungal gr...
journal_title：Current microbiology
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doi：10.1007/s00284-014-0604-x
更新日期：2014-10-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-008-9268-8
更新日期：2009-01-01 00:00:00
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更新日期：2019-03-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-010-9803-2
更新日期：2011-03-01 00:00:00
abstract：:The psychrotrophic bacterium Aeromonas hydrophila 7966 was subjected to cold shocks from 30 degrees C to 20 degrees C, 15 degrees C, 10 degrees C, or 5 degrees C, or were incubated at low temperature to determine its adaptative response. The cell protein patterns analyzed by two-dimensional electrophoresis revealed th...
journal_title：Current microbiology
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doi：10.1007/s00284-004-4277-8
更新日期：2004-08-01 00:00:00
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journal_title：Current microbiology
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更新日期：2005-05-01 00:00:00
abstract：:In the last few years, numerous cases of multidrug-resistant Achromobacter xylosoxidans infections have been documented in immunocompromised and cystic fibrosis patients. To gain insights into the molecular mechanisms and mobile elements related to multidrug resistance in this bacterium, we studied 24 non-epidemiologi...
journal_title：Current microbiology
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更新日期：2012-12-01 00:00:00
abstract：:Colonial aggregation of Microcystis plays a key role in bloom formation. Limited studies have been reported about effects of environmental factors on the aggregation of Microcystis. Calcium is an important chemical element in water system. In this study, we investigated the effects of a low- (0.015 g l⁻¹) and a high-c...
journal_title：Current microbiology
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doi：10.1007/s00284-010-9762-7
更新日期：2011-02-01 00:00:00
abstract：:A novel Gram-negative gamma-proteobacterium, non-sporulating motile, rod or coccus-shaped bacterium designated as strain PKS7T was isolated from a sediment sample collected from Chilika Lake, Odisha, India and characterized taxonomically using a polyphasic approach. The major quinone was Q8 and major cellular fatty ac...
journal_title：Current microbiology
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更新日期：2020-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4531-8
更新日期：2005-07-01 00:00:00
abstract：:Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900462
更新日期：1999-11-01 00:00:00
abstract：:Aminopeptidases (APN) may play a role in host colonization of M. gallinarum. Characterization of endogenous APN activity suggests that the leucine APN (LAP) of M. gallinarum is a metallo-aminopeptidase activated by Mn2+ and is present in the cytosol and possibly associated with the inner leaflet of the membrane. A 1.3...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4098-1
更新日期：2004-01-01 00:00:00
