Abstract：
:Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultural and morphological characteristics, which are time-consuming and inaccurate. In this study, a semi-nested PCR method was developed using primers based on internal transcribed spacer region that is specific to A. solani. 20 isolates including 6 Alternaria species and 10 other species of common potato pathogens were used to examine the specificity of the primers. The primer set ptAsQ-F/ptAs-R was highly specific to A. solani, as a product of 251 bp was amplified only from A. solani isolates and no amplification signal was observed from other tested species. The sensitivity of this method determined using A. solani genomic DNA was 10 fg. This PCR assay was also successfully employed to detect A. solani in soil with the detection sensitivity of one conidia spore in 0.5 g of soil. To the best of our knowledge, this is the first report of molecular detection of A. solani in soil, which provides a useful tool for early and rapid detection of early blight in soil before next growing season.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Gu Q,Yang ZH,Zhao DM,Zhang D,Wang Q,Ma LS,Zhu JHdoi
10.1007/s00284-017-1284-0subject
Has Abstractpub_date
2017-09-01 00:00:00pages
1083-1088issue
9eissn
0343-8651issn
1432-0991pii
10.1007/s00284-017-1284-0journal_volume
74pub_type
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