Abstract：
:In the present work, Aspergillus fumigatus is described as a higher producer of hydrolytic enzymes secreted in response to the presence of the Callosobruchus maculatus bruchid pest. This fungus was able to grow over cowpea weevil shells as a unique carbon source, secreting alkaline proteolytic and chitinolytic enzymes. Enzyme secretion in A. fumigatus was induced by both C. maculatus exoskeleton as well as commercial chitin, and alkaline proteolytic and chitinolytic activities were detected after 48 hours of growth. Furthermore, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed the production of specific proteins. Among them, two extracellular alkaline proteinases from culture enriched with C. maculatus exoskeleton were purified after chromatographic procedures using ion exchange and affinity columns. These proteins, named AP15 and AP30, had apparent molecular masses of 15,500 and 30,000 Da, respectively, as estimated by SDS-PAGE electrophoresis and mass spectrometry. AP30 was classified as a serine proteinase because it was inhibited by 5 mM: phenylmethylsulfonyl fluoride (100%) and 50 microM leupeptin (67.94%).
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Pereira JL,Franco OL,Noronha EFdoi
10.1007/s00284-005-0192-xsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
430-4issue
6eissn
0343-8651issn
1432-0991journal_volume
52pub_type
杂志文章abstract：:A genetic fingerprinting analysis of Caribbean isolates of M. avium complex (MAC) from AIDS patients by a Southern blotting technique after Pstl digestion with nonradioactive DNA probes coding for single-copy sequences DT1 and DT6 was performed. In parallel, a selective amplification of a 187-bp fragment within the DT...
journal_title：Current microbiology
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journal_title：Current microbiology
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journal_title：Current microbiology
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journal_title：Current microbiology
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journal_title：Current microbiology
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journal_title：Current microbiology
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更新日期：2013-10-01 00:00:00
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journal_title：Current microbiology
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更新日期：2008-03-01 00:00:00
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journal_title：Current microbiology
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更新日期：2020-11-01 00:00:00
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journal_title：Current microbiology
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更新日期：2017-04-01 00:00:00
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journal_title：Current microbiology
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journal_title：Current microbiology
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更新日期：2013-12-01 00:00:00
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journal_title：Current microbiology
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更新日期：2001-02-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章,评审
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更新日期：2014-11-01 00:00:00
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journal_title：Current microbiology
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更新日期：2013-09-01 00:00:00
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journal_title：Current microbiology
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journal_title：Current microbiology
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journal_title：Current microbiology
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更新日期：1998-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0031-7
更新日期：2002-02-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0578-4
更新日期：2007-09-01 00:00:00
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journal_title：Current microbiology
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更新日期：2011-02-01 00:00:00
