Proteomic analysis of protein expression in the induction of the viable but Nonculturable State of Vibrio harveyi SF1.

Abstract:

:Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cytosolic proteins were resolved by two-dimensional polyacrylamide gel electrophoresis using pH 4-7 linear gradients. Among these proteins, sixteen proteins which were strongly downregulated or upregulated in the VBNC cells were identified by MALDI-TOF-TOF mass spectrometry. The results indicated that the differentially expressed proteins were mainly focused on stress response proteins and key components of central and intermediary metabolism, like carbohydrate metabolism, transport, and translation. This study provided clues for understanding the mechanism of adaptation to the VBNC state.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Jia J,Li Z,Cao J,Jiang Y,Liang C,Liu M

doi

10.1007/s00284-013-0383-9

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

442-7

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

67

pub_type

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