Abstract:
:Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cytosolic proteins were resolved by two-dimensional polyacrylamide gel electrophoresis using pH 4-7 linear gradients. Among these proteins, sixteen proteins which were strongly downregulated or upregulated in the VBNC cells were identified by MALDI-TOF-TOF mass spectrometry. The results indicated that the differentially expressed proteins were mainly focused on stress response proteins and key components of central and intermediary metabolism, like carbohydrate metabolism, transport, and translation. This study provided clues for understanding the mechanism of adaptation to the VBNC state.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Jia J,Li Z,Cao J,Jiang Y,Liang C,Liu Mdoi
10.1007/s00284-013-0383-9subject
Has Abstractpub_date
2013-10-01 00:00:00pages
442-7issue
4eissn
0343-8651issn
1432-0991journal_volume
67pub_type
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