Abstract:
:A 11.2-kb fragment containing the ectABC genes of the biosynthetic pathway of ectoine from the Gram-positive, moderately halophilic bacterium Halobacillus dabanensis D-8(T) was obtained by inverse polymerase chain reaction. Subsequently, the entire ectABC cluster was cloned and analyzed. It revealed that the intergenic regions of the ectABC genes from H. dabanensis D-8(T) are more tightly spaced than those of Chromohalobacter salexigens, Halomonas elongata, Marinococcus halophilus, and Salibacillus pasteurii. The amino-acid sequence deduced from ectABC was highly homologous that from Virgibacillus pantethenticus (EctA 52%, EctB 60%, EctC 67%, respectively). The ectABC genes were cloned in the expression plasmid pMXB10 resulting in pMXB10ectABC. The ectoine was detected from cell extract in Escherishia coli ER2566 containing pMXB10ectABC using (13)C nuclear magnetic resonance spectroscopy.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Zhao B,Lu W,Yang L,Zhang B,Wang L,Yang SSdoi
10.1007/s00284-005-0396-0subject
Has Abstractpub_date
2006-09-01 00:00:00pages
183-8issue
3eissn
0343-8651issn
1432-0991journal_volume
53pub_type
杂志文章abstract::Vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. A gene (hlyIII) encoding a hemolysin was cloned and sequenced from V. vulnificus. Nucleotide sequence analysis predicted an open reading frame of 642 bp encodin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4288-5
更新日期:2004-09-01 00:00:00
abstract::Candida galli strain PGO6 isolated from oil-contaminated water is the first isolated yeast strain which is capable to form vanillin and vanillic acid during isoeugenol biotransformation. The products were confirmed by thin-layer chromatography (TLC), changes in the UV absorption pattern and high-performance liquid chr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9815-y
更新日期:2011-03-01 00:00:00
abstract::The influence of Bacillus thuringiensis subsp. kurstaki HD-1 spores upon the toxicity of purified Cry1Ab and Cry1C crystal proteins toward susceptible and BT-resistant Indianmeal moth (IMM, Plodia interpunctella) larvae was investigated. With susceptible larvae, HD-1 spores were toxic in the absence of crystal protein...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900074
更新日期:1996-07-01 00:00:00
abstract::Two strains of a novel basidiomycetous yeast species were isolated from the gut of wood-boring larvae collected in the Baotianman Nature Reserve, the central China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) regions showed that these yeasts belong ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0203-7
更新日期:2012-11-01 00:00:00
abstract::Thirty-one Listeria strains were tested for sensitivity to four class IIa bacteriocins, namely, enterocin A, mesentericin Y105, divercin V41, and pediocin AcH, and to nisin A. Class IIa bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, wherea...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010081
更新日期:2000-07-01 00:00:00
abstract::To investigate whether Bombyx mori immunized with Bacillus subtilis spore displaying GP64 escape from the B. mori nucleopolyhedrovirus (BmNPV) attack, a recombinant integrative plasmid named pJS700-GP64 was constructed, which carries a recombinant cotC-Gp64 gene under the control of the cotC promoter. In this study, p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9867-7
更新日期:2011-05-01 00:00:00
abstract::Genetic engineering of microbes for commercial metabolite production traditionally has sought to alter the levels and/or intrinsic activities of key enzymes in relevant biosynthetic pathway(s). Microorganisms exploit similar strategies for flux control, but also coordinate flux through sets of related pathways by usin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010255
更新日期:2001-07-01 00:00:00
abstract::The establishment of human flora-associated animal models allows the in vivo manipulation of host, microbial, and environmental parameters to influence the gut microbial community. However, it is difficult to simulate infant gut microbiota in germ-free animals because of the variation and dynamic state of infant micro...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0360-3
更新日期:2013-09-01 00:00:00
abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) displays important functional diversity in mammalian and plants. So far, however, studies on GAPDH have not included the halotolerant, unicellular green alga Dunaliella salina. In the present study, a GAPDH cDNA was cloned and sequenced from D. salina. It was 1394 bp lo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9333-3
更新日期:2009-05-01 00:00:00
abstract::On the basis of the pulsed field gel electrophoresis(PFGE) technique, molecular karyotypes in Amoeboaphelidiumprotococcarum (the endotrophic parasite of chlorophycean algae, whichcombines protozoon- and fungus-type characters) were determined. Molecularkaryotypes in the strains X1, X5, and X31, which differ in the hos...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900155
更新日期:1997-02-01 00:00:00
abstract::The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were chara...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006784
更新日期:1999-03-01 00:00:00
abstract::Enterovirus 71 (EV71) is the main pathogen of the hand, foot, and mouth disease. It was firstly isolated from sputum specimens of infants with central nervous system diseases in California in 1969, and has been repeatedly reported in various parts of the world, especially in the Asia-Pacific region. EV71 3C protein is...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-020-02082-4
更新日期:2020-09-01 00:00:00
abstract::Recently, we showed that the southern green stink bug (SGSB), Nezara viridula (L.), can transmit Pantoea agglomerans (Ewing and Fife), an opportunistic bacterium, into green cotton bolls resulting in plant disease. Here, we hypothesized that our established model could be used to determine if the SGSB was a general, n...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9452-5
更新日期:2009-10-01 00:00:00
abstract::A novel leucine aminopeptidase was purified from a Bacillus thuringiensis israelensis (Bti) culture. The purification stages included heating the concentrated supernatant to 65 degrees C for 90 min, anion-exchange chromatography by DEAE cellulose, and hydrophobic chromatography by phenyl Sepharose. The specific activi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9004-9
更新日期:2007-11-01 00:00:00
abstract::By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00294703
更新日期:1995-12-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis (SE) infection in humans is often associated with the consumption of contaminated poultry products. Binding of the bacterium to the intestinal mucosa is a major pathogenic mechanism of Salmonella in poultry. Transposon mutagenesis identified flgC as a potential binding mutant of...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0521-z
更新日期:2014-05-01 00:00:00
abstract::The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected str...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1428-2
更新日期:2018-05-01 00:00:00
abstract::A single strain, designated BF49(T), was isolated from a biofilm of a tufa deposit from the Westerhöfer rivulet, Lower Saxony, Germany. The G+C content of the genomic DNA of strain BF49(T) was 69 mol% and the predominant ubiquinone was Q-8. Major fatty acids were C(16:1)omega7c/15 iso 2OH and C(16:0). Comparative 16S ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9095-3
更新日期:2008-04-01 00:00:00
abstract::Pasteurella haemolytica (Ph) is the most important cause of the bovine acute fibrinohemorrhagic pneumonia that occurs in market stressed calves after shipment to feedyards. Recent characterization of neuraminidase production by these organisms has shown that all 16 serotypes produce an immunologically similar form of ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900372
更新日期:1998-10-01 00:00:00
abstract::In the present work, Aspergillus fumigatus is described as a higher producer of hydrolytic enzymes secreted in response to the presence of the Callosobruchus maculatus bruchid pest. This fungus was able to grow over cowpea weevil shells as a unique carbon source, secreting alkaline proteolytic and chitinolytic enzymes...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0192-x
更新日期:2006-06-01 00:00:00
abstract::The dinI homolog of S. marcescens was cloned from a plasmid library by virtue of its ability to inhibit nuclease expression from the S. marcescens nucA gene integrated in the genome of E. coli. The S. marcescens DinI protein is 68% identical to DinI of E. coli. It has a similar effect on other SOS regulated genes and ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0072-y
更新日期:2002-01-01 00:00:00
abstract::The diversities of soil bacterial communities in the Phragmites australis, Phalaris arundinacea, and non-plantation constructed wetlands were compared and analyzed by through high-throughput Illumina sequencing. At the phylum level, the ten dominant bacterial phyla in the three types of constructed wetlands were the s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01634-7
更新日期:2019-03-01 00:00:00
abstract::A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% iden...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4467-z
更新日期:2006-04-01 00:00:00
abstract::The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patie...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9239-0
更新日期:2008-12-01 00:00:00
abstract::Bacteria were isolated from rabbit faeces using equine caecal fluid as a growth medium. Two new isolates of the genus Streptococcus are described in terms of their biochemical properties. One of these has a 16S rRNA gene with 97.7%, and the other 98.5%, identity to Streptococcus thoraltensis. While S. thoraltensis has...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9619-0
更新日期:2010-10-01 00:00:00
abstract::Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900421
更新日期:1999-08-01 00:00:00
abstract::The aim of the research was (i) to evaluate white and cream strains of A. bisporus yields when challenged or not with the pathogen L. fungicola, (ii) to identify the more aggressive pathogen isolates, and (iii) to develop a diagrammatic scale of spot symptoms on infected mushrooms. The experiment was carried out using...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02232-8
更新日期:2020-12-01 00:00:00
abstract::Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9158-0
更新日期:2008-08-01 00:00:00
abstract::Lipids contribute significantly to the pathogenesis of fungal infectious diseases and an understanding of lipid metabolism occurring in fungal pathogens can help the development of more efficient antifungal therapeutic strategies. In this study, the effect of culture age on the distribution of fatty acids among differ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9052-1
更新日期:2008-02-01 00:00:00
abstract::Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900462
更新日期:1999-11-01 00:00:00