Display of Bombyx mori nucleopolyhedrovirus GP64 on the Bacillus subtilis spore coat.

Abstract:

:To investigate whether Bombyx mori immunized with Bacillus subtilis spore displaying GP64 escape from the B. mori nucleopolyhedrovirus (BmNPV) attack, a recombinant integrative plasmid named pJS700-GP64 was constructed, which carries a recombinant cotC-Gp64 gene under the control of the cotC promoter. In this study, pJS700-GP64 was transformed into B. subtilis 168 (trp(-)) competent cells, an amylase (amyE) inactivated mutant was selected, and was confirmed to be a double cross-over integrant, cotC-Gp64 fragment of which was integrated into B. subtilis chromosome. Gp64 was expressed on the spore surface and recognized by Gp64-specific antibody. Results of B. mori when challenged with BmNPV indicated that B. mori vaccinated with the recombinant spores possessed resistance to the invasion of BmNPV at some degree.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Li G,Tang Q,Chen H,Yao Q,Ning D,Chen K

doi

10.1007/s00284-011-9867-7

subject

Has Abstract

pub_date

2011-05-01 00:00:00

pages

1368-73

issue

5

eissn

0343-8651

issn

1432-0991

journal_volume

62

pub_type

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