Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis.

Abstract:

:By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidiosa genome that contains a 28-nucleotide insertion, and single base changes that distinguish CVC and grape X. fastidiosa strains. When using RAPD products to develop specific PCR primers, we found it most efficient to screen for size differences among RAPD products rather than presence/absence of a specific RAPD band.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Pooler MR,Hartung JS

doi

10.1007/BF00294703

subject

Has Abstract

pub_date

1995-12-01 00:00:00

pages

377-81

issue

6

eissn

0343-8651

issn

1432-0991

journal_volume

31

pub_type

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