Abstract:
:By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidiosa genome that contains a 28-nucleotide insertion, and single base changes that distinguish CVC and grape X. fastidiosa strains. When using RAPD products to develop specific PCR primers, we found it most efficient to screen for size differences among RAPD products rather than presence/absence of a specific RAPD band.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Pooler MR,Hartung JSdoi
10.1007/BF00294703subject
Has Abstractpub_date
1995-12-01 00:00:00pages
377-81issue
6eissn
0343-8651issn
1432-0991journal_volume
31pub_type
杂志文章abstract::Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900462
更新日期:1999-11-01 00:00:00
abstract::The diversities of soil bacterial communities in the Phragmites australis, Phalaris arundinacea, and non-plantation constructed wetlands were compared and analyzed by through high-throughput Illumina sequencing. At the phylum level, the ten dominant bacterial phyla in the three types of constructed wetlands were the s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01634-7
更新日期:2019-03-01 00:00:00
abstract::Growth on aniline by three purple non-sulfur bacteria (Rhodospirillum rubrum ATCC 11170, Rhodobacter sphaeroides DSM 158, and Rubrivivax benzoatiliticus JA2) as nitrogen, or carbon source could not be demonstrated. However in its presence, production of indole derivatives was observed with all the strains tested. At l...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9609-2
更新日期:2010-10-01 00:00:00
abstract::Aminopeptidases (APN) may play a role in host colonization of M. gallinarum. Characterization of endogenous APN activity suggests that the leucine APN (LAP) of M. gallinarum is a metallo-aminopeptidase activated by Mn2+ and is present in the cytosol and possibly associated with the inner leaflet of the membrane. A 1.3...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4098-1
更新日期:2004-01-01 00:00:00
abstract::To optimize the cell lysis step for DNA extraction from activated sludge samples, two floc dispersion methods (sonication versus stirring with a cation exchange resin), and three cell lysis treatments (lysozyme + SDS, sonication in a water bath, and thermal shock) were tested. For dispersion, stirring with cation exch...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006809
更新日期:1999-06-01 00:00:00
abstract::The genus Acinetobacter has been recognized to take up exogenous DNA from the environment. In this study, we conducted natural transformation with a novel diesel-degrading Acinetobacter sp. strain, designated strain DR1, using the broad host range plasmid pRK415. Many factors, including temperature, quantities of DNA,...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9698-y
更新日期:2011-01-01 00:00:00
abstract::Osmotically sensitive mutants of Neurospora crassa are unable to grow on medium supplemented with 4% NaCl, have altered morphologies and cell-wall compositions, and are resistant to dicarboximide fungicides. Osmotic-1 (os-1) mutants have a unique characteristic of forming protoplasts that grow and divide in specialize...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900193
更新日期:1997-06-01 00:00:00
abstract::In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0317-6
更新日期:2013-06-01 00:00:00
abstract::Two strains of the well-known insect pathogen and biopesticide, Bacillus thuringiensis (Bt), belonging to subspecies alesti (strain Bt5) and kurstaki (strain Bt213), were chosen for genetic characterization. The two strains belong to different serotypes and are currently classified into different subspecies, although ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0055-z
更新日期:2002-02-01 00:00:00
abstract::A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0495-2
更新日期:2014-04-01 00:00:00
abstract::Iron-oxidizing Acidithiobacillus spp. are applied worldwide in biomining industry to extract metals from sulfide minerals. They derive energy for survival through Fe2+ oxidation and generate Fe3+ for the dissolution of sulfide minerals. However, molecular mechanisms of their iron oxidation still remain elusive. A nove...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1453-9
更新日期:2018-07-01 00:00:00
abstract::A high-virulence clone (HVC) was proposed as causing much of the morbidity and mortality when a collection of group B Streptococcus (GBS) isolates was examined by multi-locus enzyme electrophoresis. HVC isolates could be further distinguished by their inability to grow at 40 degrees C, and a temperature-sensitive aldo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3957-5
更新日期:2003-10-01 00:00:00
abstract::Wolbachia are strictly intracellular maternally inherited alpha-proteobacteria, largely widespread among arthropods and filariids (i.e., filarial nematodes). Wolbachia capacities to infect new host species have been greatly evidenced and the transfer of Wolbachia between arthropods and filariids has probably occurred ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0578-4
更新日期:2007-09-01 00:00:00
abstract::Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacter...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0627-z
更新日期:2007-06-01 00:00:00
abstract::Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9602-9
更新日期:2010-10-01 00:00:00
abstract::In laboratory cultures of ice nucleation-active (Ice+) Erwinia herbicola isolates, it has been difficult to achieve high-level expression of ice nuclei, especially nuclei active at temperatures warmer than -5 degrees C (i.e., type 1 ice nuclei). Here we demonstrate that starvation for phosphate and exposure to low tem...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900325
更新日期:1998-06-01 00:00:00
abstract::In this study, the rumen was assessed for its potential to detoxify RDX using molecular microbial ecology as well as analytical chemistry techniques. Results indicated significant loss (P < 0.05) of RDX in <8-h post incubation, and qualitative LC-MS/MS analysis showed evidence for the formation of 1-NO-RDX (M-O + HCOO...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0144-1
更新日期:2012-08-01 00:00:00
abstract::A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02210-0
更新日期:2020-11-01 00:00:00
abstract::It has been reported that treatment with methotrexate (MTX) induces intestinal bacterial translocation; however, the definitive evidence of intestinal bacterial translocation induced by MTX has been lacking. The aim of this study was to confirm the intestinal bacterial translocation caused by MTX and to evaluate the p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0177-9
更新日期:2006-01-01 00:00:00
abstract::The spread of antibiotic resistance is rapidly threatening the effectiveness of antibiotics in the clinical setting. Many infections are being caused by known and unknown pathogenic bacteria that are resistant to many or all antibiotics currently available. Empedobacter falsenii is a nosocomial pathogen that can cause...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1498-9
更新日期:2018-08-01 00:00:00
abstract::Bats can be divided into frugivory, nectarivory, insectivory, and sanguivory based on their diets, and are therefore ideal wild animal models to study the relationship between diets and intestinal microflora. Early studies of bat gut bacteria showed that the diversity and structure of intestinal bacterial communities ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1530-0
更新日期:2018-10-01 00:00:00
abstract::A novel bacterium, designated U33(T), was isolated from a soil sample collected in Mykhailyky, Poltavs'ka oblast, Ukraine. The bacterium was aerobic, Gram-positive, spore-forming, and consists of motile rods. The taxonomic position of strain U33(T) was studied by a polyphasic approach, and the results clearly showed t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0930-7
更新日期:2016-02-01 00:00:00
abstract::Cronobacter sakazakii is now recognized as an opportunistic pathogen and has been implicated in rare but severe cases of necrotizing enterocolitis, meningitis, and sepsis in neonates. The first step in bacterial pathogenesis requires that the organism adheres to host cells surfaces; therefore, agents that inhibit adhe...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0623-7
更新日期:2014-10-01 00:00:00
abstract::Streptococcus bovis is commonly present in the rumen, but strains of S. bovis have also occasionally been isolated from human blood or fecal samples. Studies were undertaken with 16s rRNA gene sequences and DNA hybridizations to define the genetic relationships between these two groups of strains. Ruminal strains were...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00314583
更新日期:1995-11-01 00:00:00
abstract::Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main fl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1094-9
更新日期:2016-10-01 00:00:00
abstract::A Gram-negative, yellow pigmented bacterium designated strain CA5(T) that reduced selenite to elemental red selenium was isolated from soil. 16S rRNA gene sequence alignment identified the isolate as a novel Pseudomonas species with P. argentinensis, P. flavescens, and P. straminea as its closest relatives. Sequence a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9745-8
更新日期:2011-02-01 00:00:00
abstract::Nine phage isolates infectious for Vibrio vulnificus and falling into four morphological groups were isolated from estuarine waters collected in Louisiana. Of the 60 V. vulnificus strains tested, 87% were susceptible to one or more of the isolates. With the exception of V. fluvialis, Vibrio species other than vulnific...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00369859
更新日期:1995-06-01 00:00:00
abstract::We tested the hypothesis that supplementation with protein improves fermentation parameters without damaging the rumen microbial populations of beef cattle grazing Urochloa brizantha cv. Marandu during the dry season. Four rumen-cannulated Nellore bulls (571 ± 31 kg of body weight) were used in a 4 × 4 Latin square de...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01631-w
更新日期:2019-03-01 00:00:00
abstract::In November 2007, 450 m(3) of treated wastewater leaked into the drinking water distribution system contaminating the drinking water of over 10,000 inhabitants of Nokia, Southern Finland. Nearly 1,000 people visited the health centre because of gastroenteritis during the following 5 weeks. A wide range of enteric path...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9832-x
更新日期:2011-04-01 00:00:00
abstract::A lytic Pseudomonas aeruginosa phage vB_PaeP_LP14 belonging to the family Podoviridae was isolated from infected mink. The microbiological characterization revealed that LP14 was stable at 40 to 50 °C and stable over a broad range of pH (5 to 12). The latent period was 5 min, and the burst size was 785 pfu/infected ce...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02011-5
更新日期:2020-09-01 00:00:00