Abstract：
:A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0-8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular polar lipids were phosphatidylethanolamine and unidentified amino- and polar-lipids. The predominant cellular fatty acids were C18:0, iso-C14:0 and iso-C15:0. Phylogenetic analysis of 16S rRNA gene sequence revealed the nearest neighbours of strain YsT to be members of the Ohtaekwangia and Chryseolinea genera with 91.2-92.1% sequence similarity. The percentages of conserved proteins (POCP) between the genomes of YsT and related strains were less than 50%. Phenotypic analyses suggested that YsT could not metabolize glucose and related sugars, which was discriminative from its phylogenetic relatives. We, therefore, propose a novel species in a new genus, Chryseotalea sanaruensis gen. nov., sp. nov. in the family Cytophagaceae (= JCM 30318T = LMG 30359T), based on cell size, the predominant cellular fatty acid composition, and the DNA GC content (38.9 mol%).
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Maejima Y,Iino T,Muraguchi Y,Fukuda K,Ohkuma M,Suzuki T,Moriuchi R,Dohra H,Kimbara K,Shintani Mdoi
10.1007/s00284-019-01823-4subject
Has Abstractpub_date
2020-02-01 00:00:00pages
306-312issue
2eissn
0343-8651issn
1432-0991pii
10.1007/s00284-019-01823-4journal_volume
77pub_type
杂志文章abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) displays important functional diversity in mammalian and plants. So far, however, studies on GAPDH have not included the halotolerant, unicellular green alga Dunaliella salina. In the present study, a GAPDH cDNA was cloned and sequenced from D. salina. It was 1394 bp lo...
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