Genotypic characterization and phylogenetic relations of Pseudomonas sp. (Formerly P. stutzeri) OX1.

Abstract:

:Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment length polymorphism (MERFLP) analysis suggested that Pseudomonas sp. OX1 could not be classified as P. stutzeri. Phylogenetic analyses based on 16S rRNA and gyrB genes further confirmed that this strain belongs to the Pseudomonas (sensu stricto) genus, but not to the stutzeri species. The data obtained demonstrated that Pseudomonas sp. OX1 belongs to intrageneric cluster II and is related to the P. fluorescens-P. syringae complex.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Radice F,Orlandi V,Massa V,Cavalca L,Demarta A,Wood TK,Barbieri P

doi

10.1007/s00284-005-0355-9

keywords:

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

395-9

issue

5

eissn

0343-8651

issn

1432-0991

journal_volume

52

pub_type

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