Abstract：
:To investigate the correlation between mutations in promoter, attenuator, and the AmpC enzyme overproduction in Escherichia coli. ampC Promoters from 4 Escherichia coli clinical isolates were cloned upstream to the chloramphenicol acetyltransferase (CAT) gene in pCAT3 reporter plasmid. Promoter strengths were measured by chloramphenicol MIC and gene sequencing was done on the cloned ampC promoter and attenuator. The strength of promoters from AmpC hyperproducers were 8- to 64-fold higher than those from a low-level AmpC producers. In one of the high-strength promoters, the mutations were located at positions -32, +22, +26, +32 (attenuator), -76, and +79. In another promoter, the mutations were located at positions -88, -82, -18, -1, and +58. In the third promoter, mutations were found at positions -1, +58, -80, -73, -28, and +82. Mutations in Escherichia coli promoter and attenuator sequences promoted Chloramphenicol MICs, which may be the primary causal mechanism for resistance to beta-lactams antibiotics.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Yu W,Bing L,Zhenhua Ldoi
10.1007/s00284-009-9426-7subject
Has Abstractpub_date
2009-09-01 00:00:00pages
244-7issue
3eissn
0343-8651issn
1432-0991journal_volume
59pub_type
杂志文章abstract：:Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9602-9
更新日期：2010-10-01 00:00:00
abstract：:A Gram-negative, yellow pigmented bacterium designated strain CA5(T) that reduced selenite to elemental red selenium was isolated from soil. 16S rRNA gene sequence alignment identified the isolate as a novel Pseudomonas species with P. argentinensis, P. flavescens, and P. straminea as its closest relatives. Sequence a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9745-8
更新日期：2011-02-01 00:00:00
abstract：:In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biof...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1080-2
更新日期：2016-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1530-0
更新日期：2018-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910036
更新日期：2000-03-01 00:00:00
abstract：:A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01577445
更新日期：1994-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02232-8
更新日期：2020-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910016
更新日期：2000-02-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010287
更新日期：2001-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1105-x
更新日期：2016-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01570863
更新日期：1993-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0021-9
更新日期：2002-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-014-0651-3
更新日期：2014-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9692-4
更新日期：2011-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9786-z
更新日期：2011-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00298381
更新日期：1995-10-01 00:00:00
abstract：:Methanogenesis from furfural by defined mixed cultures was studied. Under sulfate-reducing conditions, a Desulfovibrio strain was used as the furfural-degrading species producing acetic acid. This sulfate-reducing bacterium (SRB) Desulfovibrio strain B is an incomplete oxidizer, unable to carry out the terminal oxidat...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0010-z
更新日期：2002-06-01 00:00:00
abstract：:The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region we...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0578-8
更新日期：2014-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1019-7
更新日期：2016-06-01 00:00:00
abstract：:In Neurospora crassa, multinucleate macroconidia are used for genetic transformation. The barrier for such a transformation can be either at the cell membrane level or at the nuclear membrane level. For assessment of these possibilities, a forced heterokaryon (containing two genetically marked nuclei and auxotrophic f...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0004-x
更新日期：2002-05-01 00:00:00
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doi：10.1007/s00284-005-0079-x
更新日期：2006-02-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9107-y
更新日期：2008-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01833-2
更新日期：2020-03-01 00:00:00
abstract：:A Gram-stain-negative, rod-shaped bacterial strain JS15-10A1T was isolated from oil production water. Its optimum growth was observed at 37 °C, pH 7.0, and 3% (w/v) NaCl. The 16S rRNA gene sequence of strain JS15-10A1T showed the highest similarities with Pseudomonas parafulva CB-1T (97.6%) and P. fulva IAM 1529T (97....
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-019-01798-2
更新日期：2020-04-01 00:00:00
abstract：:This report presents results from a study on the bacterial diversity of cigarette brands collected from military personnel during the U.S. Army's investigation of a series of cases of acute eosinophilic pneumonitis in military personnel deployed in Operation Iraqi Freedom. Eight species of Bacillus, including five new...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4491-z
更新日期：2005-07-01 00:00:00
abstract：:Acinetobacter baumannii is an emerging opportunistic pathogen that primarily infects critically ill patients in nosocomial settings and there is a need for identifying new alternative therapeutic agents against these organisms. Ceragenins are non-peptide, membrane-active agents that mimic the antimicrobial properties ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01949-w
更新日期：2020-08-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9608-3
更新日期：2010-10-01 00:00:00
abstract：:The organization of the clusters of genes encoding proteins of the botulinum neurotoxin (BoNT) progenitor complex was elucidated in a strain of Clostridium botulinum producing type B and F neurotoxins. With PCR and sequencing strategies, the type B BoNT-gene cluster was found to be composed of genes encoding BoNT/B, n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900384
更新日期：1998-11-01 00:00:00
abstract：:Growth on aniline by three purple non-sulfur bacteria (Rhodospirillum rubrum ATCC 11170, Rhodobacter sphaeroides DSM 158, and Rubrivivax benzoatiliticus JA2) as nitrogen, or carbon source could not be demonstrated. However in its presence, production of indole derivatives was observed with all the strains tested. At l...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9609-2
更新日期：2010-10-01 00:00:00
abstract：:Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1225-y
更新日期：2017-05-01 00:00:00
