Adherence of Lactobacillus to intestinal 407 cells in culture correlates with fibronectin binding.

Abstract:

:Lactobacilli are members of the normal mucosal microflora of most animals. Many isolates of Lactobacillus spp. are adherent to epithelial cells. In this study, using Lactobacillus acidophilus and L. agilis, we detected adherence in a pattern that suggested that the bacteria were binding to extracellular matrix proteins. Fluorescent microscopy, by using anti-fibronectin antibody, demonstrated that the isolates localize in those areas where fibronectin was detected. In addition, fibronectin pretreatment of the bacterial cells decreased adherence to Intestinal 407 epithelial cell monolayers. Cellular binding to fibronectin was detected by enzyme-linked immunosorbent assay and affinity binding to radio-labeled fibronectin. Fibronectin may be one of the eukaryotic receptors mediating attachment of Lactobacillus to mucosal surfaces.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Kapczynski DR,Meinersmann RJ,Lee MD

doi

10.1007/s002840010107

keywords:

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

136-41

issue

2

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284000

journal_volume

41

pub_type

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