Abstract:
:Magnetotactic bacteria (MTB) are capable of synthesizing nano-sized, intracellular membrane-bound magnetosomes. To learn more about the genetic factors involved in magnetosome formation, transposon mutagenesis was carried out by conjugation using a hyperactive mariner transposon to obtain nonmagnetic mutants of Magnetospirillum magneticum AMB-1. A mutant with defect in uvrA gene encoding the DNA binding subunit of the UvrABC complex responsible for the process of nucleotide excision repair, was obtained. Growth, magnetosome formation and maintenance of magnetosome island (MAI) were further analyzed in the absence of UvrA. Interruption of uvrA led to decreased capacity to form magnetosome when cultured in the presence of oxygen. The deficiency in UvrA also resulted in an accelerated loss of the MAI under aerobic conditions indicating that the nucleotide excision repair system guards against the instability of the MAI. The incapacity of MTB to efficiently initiate recombination mediated by RecA rescued the instability of MAI observed in uvrA mutant. Elevated recombination activity resulting from the accumulation of unrepaired mutations may thus account for the instability of MAI in the absence of UvrA.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Bo T,Wang K,Ge X,Chen G,Liu Wdoi
10.1007/s00284-012-0131-6subject
Has Abstractpub_date
2012-07-01 00:00:00pages
98-107issue
1eissn
0343-8651issn
1432-0991journal_volume
65pub_type
杂志文章abstract::Invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. Candida albicans is the main pathological agent followed by Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis. These pathogens colonize different host tissues in humans as they are able t...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-014-0651-3
更新日期:2014-11-01 00:00:00
abstract::Pathogens have evolved an array of strategies to establish a productive infection. The extracellular proteins secreted by pathogens are one of unique mechanisms to evade the host innate immune response. Many secretory proteins transported by the bacterial secretion systems have been widely investigated in Salmonella. ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02201-1
更新日期:2020-11-01 00:00:00
abstract::The lipid modification of membrane proteins was investigated in Acholeplasma laidlawii by metabolic labeling and by chemical analysis. A S-glycerylcysteine residue was identified from membrane proteins and we reported the strong preference for saturated acyl chains into the lipid modification. Differential release of ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010332
更新日期:2001-12-01 00:00:00
abstract::Lactobacilli are members of the normal mucosal microflora of most animals. Many isolates of Lactobacillus spp. are adherent to epithelial cells. In this study, using Lactobacillus acidophilus and L. agilis, we detected adherence in a pattern that suggested that the bacteria were binding to extracellular matrix protein...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010107
更新日期:2000-08-01 00:00:00
abstract::Thirty-one Listeria strains were tested for sensitivity to four class IIa bacteriocins, namely, enterocin A, mesentericin Y105, divercin V41, and pediocin AcH, and to nisin A. Class IIa bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, wherea...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010081
更新日期:2000-07-01 00:00:00
abstract::The glucose 1-phosphate uridylyltransferase (GalU) is absolutely required for the biosynthesis of capsular polysaccharide, the sine qua non virulence factor of Streptococcus pneumoniae. The pneumococcal GalU protein was overexpressed in Escherichia coli, and purified. GalU showed a pI of 4.23, and catalyzed the revers...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4466-0
更新日期:2005-10-01 00:00:00
abstract::To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9334-2
更新日期:2009-03-01 00:00:00
abstract::Predation by Bdellovibrio-like organisms (BLOs) results in bacterial community succession in aquatic ecosystems. The effects of nutrient loading on the distribution and phylogeny of BLOs remain largely unknown. To this end, we present our findings on BLO diversity from four north-Indian lakes that are variable in thei...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9385-z
更新日期:2009-07-01 00:00:00
abstract::Coral mucus-degrading bacteria were isolated by an enrichment culture procedure. The isolates were able to grow as pure cultures on 10% sterilized mucus in seawater, yielding 10(8) CFU/ml. The isolates, mostly Vibrio strains, were classified by classical and molecular methods. When carbon, nitrogen, and phosphorus com...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9100-5
更新日期:2008-05-01 00:00:00
abstract::Triterpenoid saponin derivatives oleanolic acid (OA) and ursolic acid (UA), but not betulinic acid (BA), were previously found to have strong antimicrobial activity against Streptococcus mutans. OA and UA inhibited the transcription of genes related to peptidoglycan biosynthesis, thereby preventing bacterial growth. H...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1344-5
更新日期:2018-01-01 00:00:00
abstract::The taxonomic position of bacterial strain, designated 15J9-4T, recovered from a beach soil sample on Jeju Island, South Korea, was established using a polyphasic approach. Strain 15J9-4T was assigned to phylum Bacteroidetes within the family Cytophagaceae based on 16S rRNA gene similarities. The closest phylogenetic ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1408-6
更新日期:2018-04-01 00:00:00
abstract::Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgr1p depletion on cell growth. Tagging Cgr1p with the gre...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010180
更新日期:2001-01-01 00:00:00
abstract::Quorum sensing is a system of stimuli and response correlated to population density and involves in pathogen infection, colonization, and pathogenesis. Quorum quenching enzymes as quorum sensing inhibitors have been identified in a number of bacteria and been used to control by triggering the pathogenic phenotype. The...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01739-z
更新日期:2019-12-01 00:00:00
abstract::Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14-60 days a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0237-x
更新日期:2013-02-01 00:00:00
abstract::Although extensive information is available on the effect ultraviolet (UV) radiation has on Gram-negative marine bacteria, there is a scarcity of data concerning UV radiation and Gram-positive marine bacteria. The focus of this paper is on Microbacterium maritypicum, with the Gram-negative Vibrio natriegens being used...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0349-2
更新日期:2007-07-01 00:00:00
abstract::The 5' untranslated region and the orf1 sequence from the cry2Aa1 operon from Bacillus thuringiensis subsp. kurstaki NRD-12 were sequenced and compared to that from strain HD-1. The start codon described in HD-1 does not yield in NRD-12 a protein of the expected size of 20 kDa, but a 10-amino acid peptide. A second, h...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006828
更新日期:1999-07-01 00:00:00
abstract::Bacteria were isolated from rabbit faeces using equine caecal fluid as a growth medium. Two new isolates of the genus Streptococcus are described in terms of their biochemical properties. One of these has a 16S rRNA gene with 97.7%, and the other 98.5%, identity to Streptococcus thoraltensis. While S. thoraltensis has...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9619-0
更新日期:2010-10-01 00:00:00
abstract::The microbiota in solar salterns plays an important role in salt production quantitatively and qualitatively. Bohai Bay coast is the major sea salt producing area in China. However, few ecological characterization studies of the Bohai Bay salt ponds, particularly of their microbial diversity, have been conducted. This...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0916-5
更新日期:2016-01-01 00:00:00
abstract::Nine phage isolates infectious for Vibrio vulnificus and falling into four morphological groups were isolated from estuarine waters collected in Louisiana. Of the 60 V. vulnificus strains tested, 87% were susceptible to one or more of the isolates. With the exception of V. fluvialis, Vibrio species other than vulnific...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00369859
更新日期:1995-06-01 00:00:00
abstract::A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5A(T), was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5A(T) belong to the genus Spirosoma in the family Spirosomaceae, and the highest ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0584-x
更新日期:2014-09-01 00:00:00
abstract::High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation ti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0006-8
更新日期:2002-04-01 00:00:00
abstract::Porcine parvovirus (PPV) can cause reproductive failure in swine, resulting in economic losses to the industry. Antiviral effects of diammonium glycyrrhizinate (DG) have been reported on several animal viruses; however, to date it has yet to be tested on PPV. In this study, the antiviral activity of DG on swine testis...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0540-9
更新日期:2014-07-01 00:00:00
abstract::Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006756
更新日期:1998-03-01 00:00:00
abstract::Findings show 21 fungal isolates belonging to eight genera recovered from Egyptian soils that have the potential to attack L-methionine under submerged conditions. Aspergillus flavipes had the most methioninolytic activity, giving the highest yield of L-methioninase (10.78 U/mg protein), rate of methionine uptake (93....
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9311-9
更新日期:2009-03-01 00:00:00
abstract::The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0243-z
更新日期:2013-01-01 00:00:00
abstract::The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were chara...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006784
更新日期:1999-03-01 00:00:00
abstract::Escherichia coli (E. coli) has been widely used as a host organism for producing recombinant proteins such as biocatalysts, antibody fragments, and therapeutic hormones. To enhance recombinant protein production, many E. coli strains have been genetically engineered on practical purposes. In this study, we developed t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01730-8
更新日期:2019-11-01 00:00:00
abstract::A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0-8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular pola...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01823-4
更新日期:2020-02-01 00:00:00
abstract::In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0317-6
更新日期:2013-06-01 00:00:00
abstract::The low molecular weight (LMW) heat shock protein (HSP), HSP16.6, in the unicellular cyanobacterium, Synechocystis sp. PCC 6803, protects cells from elevated temperatures. A 95% reduction in the survival of mutant cells with an inactivated hsp16.6 was observed after exposure for 1 h at 47 degrees C. Wild-type cell sur...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910056
更新日期:2000-04-01 00:00:00