Abstract:
:The fungus Mycogone perniciosa is a major pathogen of the common button mushroom Agaricus bisporus. Analysis of genetic diversity in M. Perniciosa may assist in developing methods for prophylaxis and treatment of M. Perniciosa infections. For this, it is necessary to classify M. Perniciosa into relevant class groups quickly and efficiently. Random amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR), and sequence-related amplified polymorphism (SRAP) markers were used to obtain genetic fingerprints and assess the genetic variation among 49 strains of M. perniciosa collected from different areas of Fujian Province in China. Analysis of DNA sequence polymorphism revealed two major distinct groups (Group I and Group II). Specific DNA fragments that were identified through RAPD, ISSR, and SRAP markers were sequenced and used for the designing of stable sequence-characterized amplified region (SCAR) markers. The resulting SCAR markers were then validated against the classified groups of M. perniciosa.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Wang W,Li X,Chen B,Wang S,Li C,Wen Zdoi
10.1007/s00284-016-1020-1subject
Has Abstractpub_date
2016-07-01 00:00:00pages
9-14issue
1eissn
0343-8651issn
1432-0991pii
10.1007/s00284-016-1020-1journal_volume
73pub_type
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