Analysis of Genetic Diversity and Development of SCAR Markers in a Mycogone perniciosa Population.

Abstract:

:The fungus Mycogone perniciosa is a major pathogen of the common button mushroom Agaricus bisporus. Analysis of genetic diversity in M. Perniciosa may assist in developing methods for prophylaxis and treatment of M. Perniciosa infections. For this, it is necessary to classify M. Perniciosa into relevant class groups quickly and efficiently. Random amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR), and sequence-related amplified polymorphism (SRAP) markers were used to obtain genetic fingerprints and assess the genetic variation among 49 strains of M. perniciosa collected from different areas of Fujian Province in China. Analysis of DNA sequence polymorphism revealed two major distinct groups (Group I and Group II). Specific DNA fragments that were identified through RAPD, ISSR, and SRAP markers were sequenced and used for the designing of stable sequence-characterized amplified region (SCAR) markers. The resulting SCAR markers were then validated against the classified groups of M. perniciosa.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Wang W,Li X,Chen B,Wang S,Li C,Wen Z

doi

10.1007/s00284-016-1020-1

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

9-14

issue

1

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-016-1020-1

journal_volume

73

pub_type

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