Abstract:
:The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylori and a strain with an inactive HpyIIIM were found to have rifampicin mutation frequencies of 2.93 x 10(-7) and 1.05 x 10(-7) (p > 0.05), respectively, indicating that HpyIIIM does not appear to be important in mismatch repair. Adherence of H. pylori in an in vitro model cell system was also unaffected by inactivation of HpyIIIM. Inactivation of HpyIIIM did not result in a decrease in fitness, as determined by liquid in vitro competition experiments.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Simala-Grant JL,Lam E,Keelan M,Taylor DEdoi
10.1007/s00284-004-4244-4keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
47-54issue
1eissn
0343-8651issn
1432-0991journal_volume
49pub_type
杂志文章abstract::Bacterial fish pathogens are pervasive in aquaculture. Control of bacterial fish pathogen is a difficult task among aquaculture practitioners. A large number of antibiotics are used for the control of prevalent bacterial pathogens in aquaculture. This may lead to drug resistance among pathogens and further treatment w...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1506-0
更新日期:2018-09-01 00:00:00
abstract::pH affected significantly the growth and the glucose fermentation pattern of Propionibacterium microaerophilum. In neutral conditions (pH 6.5-7.5), growth and glucose fermentation rate (qs) were optimum producing propionate, acetate, CO(2), and formate [which together represented 90% (wt/wt) of the end products], and ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3839-x
更新日期:2003-02-01 00:00:00
abstract::Variable-number-of-tandem-repeats (VNTR) markers are increasingly being used in population genetic studies of bacteria. They were recently developed for Pasteuria ramosa, an endobacterium that infects Daphnia species. In the present study, we genotyped P. ramosa in 18 infected hosts from the United Kingdom, Belgium, a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9104-1
更新日期:2008-05-01 00:00:00
abstract::The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P29...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1402-z
更新日期:2018-04-01 00:00:00
abstract::Favre and coworkers (Favre et al., Biochimie 73:235-244, 1991) previously reported that the Bordetella pertussis recA gene present at high copy number could promote a low frequency of recombination, but not bacteriophage induction in Escherichia coli RecA- mutants. Reexamination of these phenotypes demonstrated that, ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900029
更新日期:1996-03-01 00:00:00
abstract::Leucoagaricus gongylophorus, the fungus cultured by the leaf-cutting ant Atta sexdens, produces polysaccharidases that degrade leaf components by generating nutrients believed to be essential for ant nutrition. We evaluated pectinase, amylase, xylanase, and cellulase production by L. gongylophorus in laboratory cultur...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0431-1
更新日期:2006-07-01 00:00:00
abstract::The outer membrane protein of Photobacterium damsela (OMP-PD) and the gene encoding for this porin protein were isolated and characterized. The deduced amino acid sequence of the OMP-PD monomer has 338 amino acids and a calculated molecular weight of 36,951 Da. This sequence includes a 22-amino acid signal peptide at ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4111-8
更新日期:2004-03-01 00:00:00
abstract::The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. or...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1350-7
更新日期:2018-01-01 00:00:00
abstract::This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9098-8
更新日期:2008-09-01 00:00:00
abstract::Together with the worldwide Washoku (traditional Japanese foods and drinks) boom, interest in sake, a traditional Japanese alcoholic drink, is increasing around the world. There are few scientific analyses and studies on the production of sake or the final product itself. We show the diversity of bacterial contaminant...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-019-01718-4
更新日期:2020-04-01 00:00:00
abstract::One hundred fifty-five isolates of Bipolaris sorokiniana of wheat were studied for their morphopathological characterization. These isolates were grouped in five categories--black, brown/dull black, gray cottony growth, dull white/greenish black, and white--on the basis of their growth pattern. The frequency of the bl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-0035-z
更新日期:2007-08-01 00:00:00
abstract::Potassium tellurite (K2TeO3) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K2TeO3 resistance in STEC is attributable to the ter gene cluster with terD an indicator of the c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1444-x
更新日期:2018-06-01 00:00:00
abstract::To investigate whether Bombyx mori immunized with Bacillus subtilis spore displaying GP64 escape from the B. mori nucleopolyhedrovirus (BmNPV) attack, a recombinant integrative plasmid named pJS700-GP64 was constructed, which carries a recombinant cotC-Gp64 gene under the control of the cotC promoter. In this study, p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9867-7
更新日期:2011-05-01 00:00:00
abstract::Two strains of a novel basidiomycetous yeast species were isolated from the gut of wood-boring larvae collected in the Baotianman Nature Reserve, the central China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) regions showed that these yeasts belong ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0203-7
更新日期:2012-11-01 00:00:00
abstract::The dinI homolog of S. marcescens was cloned from a plasmid library by virtue of its ability to inhibit nuclease expression from the S. marcescens nucA gene integrated in the genome of E. coli. The S. marcescens DinI protein is 68% identical to DinI of E. coli. It has a similar effect on other SOS regulated genes and ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0072-y
更新日期:2002-01-01 00:00:00
abstract::The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9831-y
更新日期:2011-04-01 00:00:00
abstract::Bacteria use quorum sensing signalling in various functions, e.g. while forming biofilms, and inhibition of this signalling could be one way to control biofilm formation. The aim of this study was to evaluate the production of signalling molecules and its correlation with the biofilm formation capability of bacteria i...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0627-3
更新日期:2014-11-01 00:00:00
abstract::The dicarboxylic acids malate and fumarate increase ruminal pH, reduce methane production, increase propionate and total volatile fatty acid (VFA) production, and reduce lactic acid accumulation in a manner similar to ionophores. These acids stimulate the ruminal bacterium Selenomonas ruminantium to ferment lactate to...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9351-1
更新日期:2009-05-01 00:00:00
abstract::Biofilm-forming bacteria Pseudomonas aeruginosa is a common pathogen in mechanically ventilated newborns, which can cause life-threatening infections. Alginate of mucoid Pseudomonas aeruginosa biofilms is considered an important virulence factor which contributes to the resistance to antibiotics. Traditionally, ambrox...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9142-8
更新日期:2008-07-01 00:00:00
abstract::The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9977-2
更新日期:2011-09-01 00:00:00
abstract::A genetic fingerprinting analysis of Caribbean isolates of M. avium complex (MAC) from AIDS patients by a Southern blotting technique after Pstl digestion with nonradioactive DNA probes coding for single-copy sequences DT1 and DT6 was performed. In parallel, a selective amplification of a 187-bp fragment within the DT...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900127
更新日期:1996-12-01 00:00:00
abstract::All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02150-9
更新日期:2020-11-01 00:00:00
abstract::Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0133-8
更新日期:2006-03-01 00:00:00
abstract::In the present study, EMA (ethidium monoazide) treatment was applied to a silty-sand reference soil prior to DNA extraction to enable a differentiation between dead and living cells. For this purpose, a reference soil was spiked with Listeria monocytogenes cells or cell equivalents, respectively. With the purpose of e...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01772-y
更新日期:2019-12-01 00:00:00
abstract::Coral mucus-degrading bacteria were isolated by an enrichment culture procedure. The isolates were able to grow as pure cultures on 10% sterilized mucus in seawater, yielding 10(8) CFU/ml. The isolates, mostly Vibrio strains, were classified by classical and molecular methods. When carbon, nitrogen, and phosphorus com...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9100-5
更新日期:2008-05-01 00:00:00
abstract::The psychrotrophic bacterium Aeromonas hydrophila 7966 was subjected to cold shocks from 30 degrees C to 20 degrees C, 15 degrees C, 10 degrees C, or 5 degrees C, or were incubated at low temperature to determine its adaptative response. The cell protein patterns analyzed by two-dimensional electrophoresis revealed th...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4277-8
更新日期:2004-08-01 00:00:00
abstract::In recent years, Candida albicans infections treatment has become a growing problem because, among others, pathogenic strains are capable to develop resistance to the administered drugs. The elaboration of rapid and accurate method of resistance assessment is an important goal of many studies. They aim to avoid inappr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1039-3
更新日期:2016-08-01 00:00:00
abstract::A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase ch...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900035
更新日期:1996-04-01 00:00:00
abstract::Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not pr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3976-2
更新日期:2003-09-01 00:00:00
abstract::The antilisterial efficiency of three bacteriocins from lactic acid bacteria, lactocin 705 (produced by L. casei CRL705, 17000 AU/ml), enterocin CRL35 (produced by E. faecium CRL35, 17000 AU/ml), and nisin (2000 IU/ml), was tested in broth, individually and in combination against Listeria monocytogenes and Listeria in...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010159
更新日期:2000-12-01 00:00:00