Mycobacterium tuberculosis expresses ftsE gene through multiple transcripts.

abstract：:Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli....

journal_title：Current microbiology

authors： Specht KM,Sheetz KH,Alexander CM,Lamech LT,O'Connor LH,Walker DM,Stevenson HP

更新日期：2010-04-01 00:00:00

abstract：:Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...

journal_title：Current microbiology

authors： Guerlava P,Izac V,Tholozan JL

更新日期：1998-03-01 00:00:00

abstract：:The thermophilic fungus Thermomyces lanuginosus, which is able to use dextran as primary carbon source for growth, excreted during the early phases of growth an enzyme activity capable of degrading dextran. The activity peaked at 22 h and decreased rapidly after the culture entered the stationary phase, probably cause...

journal_title：Current microbiology

authors： Jensen B,Olsen J

更新日期：1996-09-01 00:00:00

abstract：:Sequence analysis of dnaE, hlyA, and asd housekeeping genes were used to determine the genetic relatedness of our collection of Vibrio cholerae isolated from patients and surface waters over a 5-year period in Iran. The results showed 41, 17, and 9 variable sites throughout the sequenced fragments of dnaE (837 bp), hl...

journal_title：Current microbiology

authors： Dashtbani-Roozbehani A,Bakhshi B,Pourshafie MR

更新日期：2013-07-01 00:00:00

abstract：:With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...

journal_title：Current microbiology

authors： Wilson M,Patel H,Noar JH

更新日期：1998-01-01 00:00:00

abstract：:A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...

journal_title：Current microbiology

authors： Chauhan VS,Singh B,Singh S,Bisen PS

更新日期：2001-02-01 00:00:00

abstract：:Expression from the secA gene, encoding a key component of the general secretory pathway of Escherichia coli, is influenced by the secretion status of the cell, autogenous translational repression, and translational coupling to the upstream gene, X. SecA binds to its mRNA in a region overlapping its ribosome binding s...

journal_title：Current microbiology

authors： Kiser KB,Schmidt MG

更新日期：1999-02-01 00:00:00

abstract：:A common activity in the global search for useful Cry toxins is the microscopic screening of bacterial colonies for the presence of Bacillus thuringiensis. High-throughput screens require that aliquots from large numbers of colonies be arrayed on a microscopic slide. However, precisely placing a small amount of bacter...

journal_title：Current microbiology

authors： Ammons D,Rampersad J

更新日期：2015-09-01 00:00:00

abstract：:The production of linolenic acid in mycelial lipids reached 0.31 mg/ml of culture broth when Mortierella isabellina was cultivated in a medium consisting of 2% octadecanol, 1% yeast extract, and 25 mmol/L of Mg2+ at 23 degrees C for 5 days. Cultivation conditions were studied, and the results showed that (i) a suitabl...

journal_title：Current microbiology

authors： Xian M,Kang Y,Yan J,Liu J,Bi Y,Zhen K

更新日期：2002-02-01 00:00:00

abstract：:A novel virulent phage, vB_KpnP_IME337, isolated from a hospital sewage in Beijing, China, that infects carbapenem-resistant Klebsiella pneumoniae KN2 capsular type was identified and characterized. Next-generation sequencing and genome analysis revealed that vB_KpnP_IME337 had a linear double-stranded genome with a l...

journal_title：Current microbiology

authors： Gao M,Wang C,Qiang X,Liu H,Li P,Pei G,Zhang X,Mi Z,Huang Y,Tong Y,Bai C

更新日期：2020-05-01 00:00:00

abstract：:Bifidobacteria are one of the major components in human microbiota that are suggested to function in maintaining human health. The colonization and cell number of Bifidobacterium species in human intestine vary with ageing. However, sequential changes of Bifidobacterium species ranging from newborns to centenarians re...

journal_title：Current microbiology

authors： Kato K,Odamaki T,Mitsuyama E,Sugahara H,Xiao JZ,Osawa R

更新日期：2017-08-01 00:00:00

abstract：:Interactions between Bacillus anthracis (B. anthracis) and host cells are of particular interest given the implications of anthrax as a biological weapon. Inhaled B. anthracis endospores encounter alveolar macrophages as the first line of defense in the innate immune response. Yet, the consequences of this interaction...

journal_title：Current microbiology

authors： Porasuphatana S,Cao GL,Tsai P,Tavakkoli F,Huwar T,Baillie L,Cross AS,Shapiro P,Rosen GM

更新日期：2010-12-01 00:00:00

abstract：:We studied the usefulness of flow cytometry for detection of penicillin resistance in E. faecalis and S. aureus by direct binding of commercially available fluorescent penicillin, Bocillin FL, to cells obtained from culture. There were significantly lower percentages of fluorescent cells and median and mean fluorescen...

journal_title：Current microbiology

authors： Jarzembowski T,Wiśniewska K,Józwik A,Bryl E,Witkowski J

更新日期：2008-08-01 00:00:00

abstract：:A genetic fingerprinting analysis of Caribbean isolates of M. avium complex (MAC) from AIDS patients by a Southern blotting technique after Pstl digestion with nonradioactive DNA probes coding for single-copy sequences DT1 and DT6 was performed. In parallel, a selective amplification of a 187-bp fragment within the DT...

journal_title：Current microbiology

authors： Sola C,Devallois A,Goh KS,Legrand E,Rastogi N

更新日期：1996-12-01 00:00:00

abstract：:The complex gut microbiota plays a key role in host metabolism and health. However, the core microbial communities in the different aged Bactrian camels remain totally unclear. We used high-throughput 16S rRNA gene sequencing to examine the temporal variability of the fecal microbiota in Bactrian camels. At 2 months o...

journal_title：Current microbiology

authors： He J,Hai L,Orgoldol K,Yi L,Ming L,Guo F,Li G,Ji R

更新日期：2019-07-01 00:00:00

abstract：:Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and construct...

journal_title：Current microbiology

authors： Lian C,Zhou Y,Feng F,Chen L,Tang Q,Yao Q,Chen K

更新日期：2014-04-01 00:00:00

abstract：:A Gram positive, thermophilic, and filamentous bacterium, designated strain GIESS004(T), was isolated from a sludge compost from Guangzhou, China. Growth occurred at 45-55 °C (optimum 50-53 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). The organism formed cream-yellow colonies with radial wrinkles. Aerial mycelium was not...

journal_title：Current microbiology

authors： Yu Z,Wu C,Yang GQ,Zhou SG

更新日期：2015-01-01 00:00:00

abstract：:This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...

journal_title：Current microbiology

authors： Shi Y,Wu S,Li W,Liu D,Ma G,Zhang Z,Yuan L,Li H,Zhang S,Mushtaq N,Xu J

更新日期：2020-12-01 00:00:00

abstract：:A moderately psychrophilic bacterium Corynebacterium paurometabolum MTCC 6841 (gram positive, short rod type) producing extracellular alkaline lipase was isolated from Lake Naukuchiatal, Uttaranchal, India. The bacterium was able to grow within a broad range of pH (5-10). Soyabean oil and olive oil served as the best ...

journal_title：Current microbiology

authors： Joshi GK,Kumar S,Tripathi BN,Sharma V

更新日期：2006-05-01 00:00:00

abstract：:This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...

journal_title：Current microbiology

authors： Nongkhlaw FM,Joshi SR

更新日期：2016-01-01 00:00:00

abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...

journal_title：Current microbiology

authors： Bhatti M,MacRobert A,Henderson B,Wilson M

更新日期：2002-08-01 00:00:00

abstract：:The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...

journal_title：Current microbiology

authors： Zhang H,Fan H,Lu C

更新日期：2010-12-01 00:00:00

abstract：:Lysozyme is an important and widespread component of the innate immune response that constitutes the first line of defense against bacterial pathogens. The bactericidal effect of this enzyme relies on its capacity to hydrolyze the bacterial cell wall and also on a nonenzymatic mechanism involving its cationic antimicr...

journal_title：Current microbiology

authors： Hébert L,Bidaud P,Goux D,Benachour A,Laugier C,Petry S

更新日期：2014-03-01 00:00:00

abstract：:Bradysia difformis is one of the most damaging pests in mushroom production in China. In this study, eight Bacillus thuringiensis strains were analyzed for insecticidal activity in B. difformis. The strain JW-1 showed the highest insecticidal activity against B. difformis larvae, but did not inhibit the mycelial growt...

journal_title：Current microbiology

authors： Wang FF,Qu SX,Lin JS,Li HP,Hou LJ,Jiang N,Luo X,Ma L

更新日期：2020-10-01 00:00:00

abstract：:Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...

journal_title：Current microbiology

authors： Janda JM,Abbott SL,Khashe S

更新日期：1999-08-01 00:00:00

abstract：:A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...

journal_title：Current microbiology

authors： Park S,Choi J,Park JM,Yoon JH

更新日期：2018-11-01 00:00:00

abstract：:The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...

journal_title：Current microbiology

authors： Garcia M,Procópio L

更新日期：2020-06-01 00:00:00

abstract：:A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogeneti...

journal_title：Current microbiology

authors： López NI,Pettinari MJ,Stackebrandt E,Tribelli PM,Põtter M,Steinbüchel A,Méndez BS

更新日期：2009-11-01 00:00:00

abstract：:Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment len...

journal_title：Current microbiology

authors： Radice F,Orlandi V,Massa V,Cavalca L,Demarta A,Wood TK,Barbieri P

更新日期：2006-05-01 00:00:00

abstract：:The diversities of soil bacterial communities in the Phragmites australis, Phalaris arundinacea, and non-plantation constructed wetlands were compared and analyzed by through high-throughput Illumina sequencing. At the phylum level, the ten dominant bacterial phyla in the three types of constructed wetlands were the s...

journal_title：Current microbiology

authors： Fu W,Wang Y,Wei W,Li P

更新日期：2019-03-01 00:00:00