Expression and characterization of penicillin-binding proteins in Burkholderia cenocepacia.

Abstract:

:Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli. The fusion proteins were isolated and shown to bind beta-lactams, indicating these putative PBPs have penicillin-binding activity.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Specht KM,Sheetz KH,Alexander CM,Lamech LT,O'Connor LH,Walker DM,Stevenson HP

doi

10.1007/s00284-009-9537-1

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

274-9

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

60

pub_type

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