Abstract：
:We studied the usefulness of flow cytometry for detection of vancomycin resistance in Enterococcus faecalis by direct binding of commercially available fluorescent vancomycin to cells obtained from culture. The cells were stained with Vancomycin@FL, sonicated and additionally stained with propidium iodide (PI). Regarding to inductive mechanism of vanA-mediated vancomycin resistance, resistant reference strain was also pre-incubated with vancomycin. PI staining divided cells into two subpopulations. There were significantly lower mean FL1 fluorescence values and mean fluorescence per particle (FL1/FSC) in reference vancomycin-resistant strain than in reference and clinical strains sensitive to this antibiotic. Pre-incubation with vancomycin of vancomycin resistant enterococci strain modified Vancomycin@FL binding, however, cells remained easy to differ. We have demonstrated new, quick and sensitive method for detection of vancomycin resistant strains of E. faecalis. The study proved possibility of detection of vancomycin resistance caused by presence of vanA gene by staining cells with Vancomycin@FL. Flow cytometry approach study of E. faecalis vancomycin resistance by detection of Vancomycin@FL binding to the bacterial cells.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Jarzembowski T,Jóźwik A,Wiśniewska K,Witkowski Jdoi
10.1007/s00284-010-9628-zsubject
Has Abstractpub_date
2010-11-01 00:00:00pages
407-10issue
5eissn
0343-8651issn
1432-0991journal_volume
61pub_type
杂志文章abstract：:The search for a vaccine capable of conferring heterologous protection, through the identification of conserved and cross-protective antigens, remains an ongoing priority in leptospirosis research. In the present study, an in silico analysis was used to identify potentially protective lipoproteins from Leptospira inte...
journal_title：Current microbiology
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journal_title：Current microbiology
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更新日期：1994-03-01 00:00:00
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journal_title：Current microbiology
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