Phage displayed Bacillus thuringiensis Cry1Ba4 toxin is toxic to Plutella xylostella.

Abstract:

:We constructed recombinant phage particles displaying the Bacillus thuringiensis Cry1Ba4 active toxin using the pfUSE5 and pComb3X phagemid vectors. The recombinant phage particles were screened and evaluated for displayed biologically active Cry1Ba4 toxin against the target insect larvae. Concurrent expression of Cry1Ba4 protoxin was carried out using the pETBlue -2 plasmid expression vector in Escherichia coli Tuner (DE3)pLacI and the protoxin was successfully expressed at a size of 129 kDa. In the bioassay, 3.30 mg crude extract of Cry1Ba4 protoxin, 9.35 x 10(9) TU and 7.70 x 10(9) TU of induced recombinant phage particles carrying Cry1Ba4 active toxin displayed on pComb3X and pFUSE5, respectively, demonstrated mortality of greater than 85% against Plutella xylostella (third-instar) within 48 hours. Thus, we have successfully displayed the Cry1Ba4 activated toxin on the surface of a phage and demonstrated toxicity towards larvae.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Nathan S,Aziz DH,Mahadi NM

doi

10.1007/s00284-006-0164-9

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

412-5

issue

5

eissn

0343-8651

issn

1432-0991

journal_volume

53

pub_type

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