Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea.

Abstract:

:Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage titers is noted at temperature 30-50 °C. A latent period of approximately 96 mins is indicated by the one-step growth curve. And, the burst size is about three virions per cell. Furthermore, genomic analysis of H188 reveals a genome size of 50364 bp with 43.63 % G+C content, and 76 putative open reading frames. There is no obvious similarity between H188 and other known phages by genomic comparison. Moreover, the H188 genome includes modules for phage structure, phage packaging, DNA replication and regulation, and some additional functions.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Li Y,Wang M,Liu Q,Song X,Wang D,Ma Y,Shao H,Jiang Y

doi

10.1007/s00284-015-0984-6

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

628-33

issue

5

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-015-0984-6

journal_volume

72

pub_type

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