Type III secretion homologs are present in Brucella melitensis, B. ovis, and B. suis biovars 1, 2, and 3.

Abstract:

:Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in PCR and hybridization assays to determine their distribution among other Brucella nomen species and biovars. The results indicated that flhB, fliP, fliR and fliF are present in Brucella melitensis, Brucella ovis, and Brucella suis biovars 1, 2 and 3. Similar homologos have been reported previously in Brucella abortus. Using RT-PCR assays, we were unable to detect any expression of these genes. It is not yet known whether the genes are the cryptic remnants of a flagellar system or are actively involved in a process contributing to pathogenicity or previously undetected motility, but they are distributed widely in Brucella and merit further study to determine their role.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Abdallah AI,Commander NJ,Woodward MJ,Spencer S,Hart CA,Winstanley C

doi

10.1007/s00284-002-3789-3

keywords:

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

241-5

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

46

pub_type

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