Abstract:
:Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in PCR and hybridization assays to determine their distribution among other Brucella nomen species and biovars. The results indicated that flhB, fliP, fliR and fliF are present in Brucella melitensis, Brucella ovis, and Brucella suis biovars 1, 2 and 3. Similar homologos have been reported previously in Brucella abortus. Using RT-PCR assays, we were unable to detect any expression of these genes. It is not yet known whether the genes are the cryptic remnants of a flagellar system or are actively involved in a process contributing to pathogenicity or previously undetected motility, but they are distributed widely in Brucella and merit further study to determine their role.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Abdallah AI,Commander NJ,Woodward MJ,Spencer S,Hart CA,Winstanley Cdoi
10.1007/s00284-002-3789-3keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
241-5issue
4eissn
0343-8651issn
1432-0991journal_volume
46pub_type
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