Abstract：
:Streptococcus bovis is commonly present in the rumen, but strains of S. bovis have also occasionally been isolated from human blood or fecal samples. Studies were undertaken with 16s rRNA gene sequences and DNA hybridizations to define the genetic relationships between these two groups of strains. Ruminal strains were found to yield genomic DNA restriction endonuclease digest patterns different from human strains when either the 16s rRNA gene amplified from ruminal S. bovis strain JB1 or a conserved universal 23s rRNA fragment was used as probes. A DNA probe based on the V1 region of the 16s rRNA of S. bovis JB1 was found to hybridize to DNAs of other ruminal S. bovis strains (K27FF4, 21-09-6C, five new ruminal isolates, and weak hybridization was found with DNAs from S. bovis 33317 (type strain), S. equinus 9812, and six other ruminal isolates. No hybridization occurred with strains representing different major human biotypes/homology groups (43143, 43144, 27960, V1387). All ruminal S. bovis strains had a guanosine plus cytosine DNA content of 37.4-38.8 mol% and, based on DNA-DNA genomic hybridizations, could be separated into two homology groups, one of which included S. equinus 9812 and S. bovis 33317. Both ruminal groups had less than 38% DNA homology to the human strains, indicating ruminal strains are clearly two separate species distinct from the human strains.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Nelms LF,Odelson DA,Whitehead TR,Hespell RBdoi
10.1007/BF00314583subject
Has Abstractpub_date
1995-11-01 00:00:00pages
294-300issue
5eissn
0343-8651issn
1432-0991journal_volume
31pub_type
杂志文章abstract：:The interactions between the proteolytic X2L strain of Oenococcus oeni and the non-proteolytic 12p strain of Pediococcus pentosaceus were assayed. The characteristics of cell growth, protein degradation, and amino acid production of both strains were determined in pure and mixed cultures. O. oeni showed poor cell grow...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0079-x
更新日期：2006-02-01 00:00:00
abstract：:A bacterium designated strain B113, able to degrade benzene, toluene, and ethylbenzene compounds (BTE), was isolated from gasoline-contaminated sediment at a gas station in Geoje, Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Acinetobacter. The biodegradati...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9268-8
更新日期：2009-01-01 00:00:00
abstract：:The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9977-2
更新日期：2011-09-01 00:00:00
abstract：:The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in th...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010199
更新日期：2001-03-01 00:00:00
abstract：:A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4-37 °C (optimum: 30 °C) and pH 4-9 (optimum: pH 6), as well as in the presence of 0-6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-sh...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-020-02296-6
更新日期：2021-01-02 00:00:00
abstract：:The regulation of the recA gene expression in the obligately anaerobic rumen bacterium Prevotella ruminicola was investigated by monitoring the recA-specific transcript level. P. ruminicola recA forms a monocistronic unit, but no SOS-box sequences resembling those of Escherichia coli or Bacillus subtilis can be identi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900306
更新日期：1998-05-01 00:00:00
abstract：:To understand the mechanism of development of cross-resistance in food pathogen Bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. Pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1090-0
更新日期：2016-10-01 00:00:00
abstract：:The yeast Candida glycerinogenes produces a high yield of glycerol only in response to a medium-osmotic stress, but little is known about the relationship between osmoadaptation and glycerol metabolism. The CgTPI gene encoding triose phosphate isomerase of C. glycerinogenes was cloned and sequenced, and its functional...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0070-9
更新日期：2007-08-01 00:00:00
abstract：:The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P29...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1402-z
更新日期：2018-04-01 00:00:00
abstract：:Nine domestic wine samples collected from a Japanese winery were examined for the presence of fumonisin B1 (FB1), fumonisin B2 (FB2), and fumonisin B3 (FB3), as well as ochratoxin A (OTA) and ochratoxin B (OTB). Wine samples spiked with 13C-labeled internal standards (13C34-FB1 and 13C20-OTA) were diluted with phospha...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02113-0
更新日期：2020-10-01 00:00:00
abstract：:A gene encoding the rice (Oryza sativa L.) 90-kDa heat shock protein (OsHsp90) was introduced into Escherichia coli using the pGEX-6p-3 expression vector with a glutathione-S-transferase (GST) tag to analyze the possible function of this protein under heat stress for the first time. We compared the survivability of E....
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9288-4
更新日期：2009-02-01 00:00:00
abstract：:AVPDS (Animal Viruses Probe dataset) is a dataset of virus-specific and conserve oligonucleotides for identification and diagnosis of viruses infecting animals. The current dataset contain 20,619 virus specific probes for 833 viruses and their subtypes and 3,988 conserved probes for 146 viral genera. Dataset of virus ...
journal_title：Current microbiology
pub_type： 信件
doi：10.1007/s00284-013-0477-4
更新日期：2014-03-01 00:00:00
abstract：:A bacterium that tested positive with antibodies specific for Escherichia coli O157 was isolated from beef during routine screening procedures. The bacterium was identified as E. fergusonii by biochemical testing and partial sequencing of 16S rRNA. The isolate was tested for the presence of genes encoding Shiga toxins...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0447-6
更新日期：2006-06-01 00:00:00
abstract：:A 11.2-kb fragment containing the ectABC genes of the biosynthetic pathway of ectoine from the Gram-positive, moderately halophilic bacterium Halobacillus dabanensis D-8(T) was obtained by inverse polymerase chain reaction. Subsequently, the entire ectABC cluster was cloned and analyzed. It revealed that the intergeni...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0396-0
更新日期：2006-09-01 00:00:00
abstract：:Although extensive information is available on the effect ultraviolet (UV) radiation has on Gram-negative marine bacteria, there is a scarcity of data concerning UV radiation and Gram-positive marine bacteria. The focus of this paper is on Microbacterium maritypicum, with the Gram-negative Vibrio natriegens being used...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0349-2
更新日期：2007-07-01 00:00:00
abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen, which can cause severe urinary tract infections (UTIs). Because of the high intrinsic antibiotic resistance of P. aeruginosa and its ability to develop new resistances during antibiotic treatment, these infections are difficult to eradicate. The antibiotic sus...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0026-y
更新日期：2012-01-01 00:00:00
abstract：:Rhodobacter sphaeroides is a bacterium that can produce hydrogen by interaction with hydrogenase and nitrogenase. We report a hydrogen production system using co-cultivation of hydrogenase in liquid medium and immobilized nitrogenase in Escherichia coli. The recombinant plasmid has been constructed to analyze the effe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0941-4
更新日期：2016-03-01 00:00:00
abstract：:The organization of the clusters of genes encoding proteins of the botulinum neurotoxin (BoNT) progenitor complex was elucidated in a strain of Clostridium botulinum producing type B and F neurotoxins. With PCR and sequencing strategies, the type B BoNT-gene cluster was found to be composed of genes encoding BoNT/B, n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900384
更新日期：1998-11-01 00:00:00
abstract：:Antibiotics are generally applied for treatment or as subtherapeutic agents to overcome diseases caused by pathogenic bacteria including Escherichia coli, Salmonella and Enterococcus species in poultry. However, due to their possible adverse effects on animal health and to maintain food safety, probiotics, prebiotics,...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02091-3
更新日期：2020-09-01 00:00:00
abstract：:Pasteurella haemolytica (Ph) is the most important cause of the bovine acute fibrinohemorrhagic pneumonia that occurs in market stressed calves after shipment to feedyards. Recent characterization of neuraminidase production by these organisms has shown that all 16 serotypes produce an immunologically similar form of ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900372
更新日期：1998-10-01 00:00:00
abstract：:The enumeration of Archaea in deep-sea sediment samples is still limited, although different methodological procedures have been applied. Among these, catalysed reporter deposition-fluorescence in situ hybridisation (CARD-FISH) technique is a promising tool for estimation of archaeal abundance in deep-sea sediment sam...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0056-5
更新日期：2012-03-01 00:00:00
abstract：:Pseudomonas aeruginosa is a serious pathogen involved in nosocomial infections. Its pathogenicity is owed to rich production of virulence factors (VIFs) regulated by several complex hierarchical signal systems depending on environmental conditions, medium composition, and the presence of certain active compounds in it...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0441-7
更新日期：2007-04-01 00:00:00
abstract：:A novel leucine aminopeptidase was purified from a Bacillus thuringiensis israelensis (Bti) culture. The purification stages included heating the concentrated supernatant to 65 degrees C for 90 min, anion-exchange chromatography by DEAE cellulose, and hydrophobic chromatography by phenyl Sepharose. The specific activi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9004-9
更新日期：2007-11-01 00:00:00
abstract：:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1217-y
更新日期：2017-04-01 00:00:00
abstract：:A Pseudomonas sp. that may be useful in bioremediation projects was isolated from soil. The strain is of potential value because it reduces selenite to elemental red selenium and is unusual in that it was resistant to high concentrations of both selenate and selenite. Exposure of the strain to 50, 100, and 150 mM sele...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9358-2
更新日期：2009-05-01 00:00:00
abstract：:Unicellular green algae, like Chlorella, offer a potentially useful system for the expression of heterologous proteins. However, the development of Chlorella as a bioreactor has been delayed owing to the lack of a stable transformation technique. Here we report on the use of micro-projectile bombardment to introduce t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900268
更新日期：1997-12-01 00:00:00
abstract：:Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1225-y
更新日期：2017-05-01 00:00:00
abstract：:Reindeer (Rangifer tarandus tarandus) may include large proportions of lichens in their winter diet. These dietary lichens are rich in phenolic secondary compounds, the most well-known being the antimicrobial usnic acid. Previous studies have shown that reindeer host rumen bacteria resistant to usnic acid and that usn...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0534-7
更新日期：2014-06-01 00:00:00
abstract：:A novel facultative anaerobic, Gram-stain-positive coccus, strain JS71T, was isolated from the human subgingival dental plaque of a periodontitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence had high sim...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01778-6
更新日期：2019-12-01 00:00:00
abstract：:A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterizatio...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900470
更新日期：1999-12-01 00:00:00
