The metabolism of aluminum citrate and biosynthesis of oxalic acid in Pseudomonas fluorescens.

Abstract:

:(13)CNMR and (1)HNMR studies revealed that aluminum citrate (Al-citrate) was metabolized intracellularly and that oxalic acid was an important product in the Al-stressed cells. This dicarboxylic acid was produced via the oxidation of glyoxylate, a precursor generated through the cleavage of isocitrate. In the control cells, citrate was biotransformed essentially with the aid of regular tricarboxylic cycle (TCA) enzymes. However, these control cells were able neither to uptake nor to metabolize Al-citrate. Al-stressed cells obtained at 38-40 h of growth showed maximal Al-citrate uptake and biotransforming activities. At least a fourfold increase in the activity of the enzyme isocitrate lyase (ICL, E. C. 4.1.3.1) has been observed in the Al-stressed cells compared with the control cells. The transport of Al-citrate was sensitive to p-dinitrophenol and sodium azide, but not to dicyclohexylcarbodiimide. Experiments with the dye 9-aminoacridine revealed that the translocation of Al-citrate led to an increase in intracellular pH. Thus, it appears that after the uptake of Al-citrate, this complex is metabolized intracellularly.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Appanna VD,Hamel RD,Lévasseur R

doi

10.1007/s00284-002-3944-x

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

32-9

issue

1

eissn

0343-8651

issn

1432-0991

journal_volume

47

pub_type

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