Direct detection and quantification of horizontal gene transfer by using flow cytometry and gfp as a reporter gene.

abstract：:Low-substrate regulated microaerophilic behavior (LSRMB) was observed in 10-54% of the bacteria isolated from several fresh-water lakes or ponds, subsurface soils, activated sludge, and Antarctic dry valley soils. Five Pseudomonas and two Bacillus type species showed LSRMB. A subsurface Pseudomonas jessenii strain was...

journal_title：Current microbiology

authors： Mazumder R,Pinkart HC,Alban PS,Phelps TJ,Benoit RE

更新日期：2000-08-01 00:00:00

abstract：:Immobilized cells of Enterobacter agglomerans, able to reduce azo dyes enzymatically, were used as a biocatalyst for the decolorization of synthetic medium containing the toxic azo dye methyl red (MR). This bacterial strain exhibits high ability to completely decolorize 100 mg/L of MR after only 6 h of incubation unde...

journal_title：Current microbiology

authors： Moutaouakkil A,Zeroual Y,Dzayri FZ,Talbi M,Lee K,Blaghen M

更新日期：2004-02-01 00:00:00

abstract：:High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation ti...

journal_title：Current microbiology

authors： Puri S,Beg QK,Gupta R

更新日期：2002-04-01 00:00:00

abstract：:The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P29...

journal_title：Current microbiology

authors： Diop K,Andrieu C,Michelle C,Armstrong N,Bittar F,Bretelle F,Fournier PE,Raoult D,Fenollar F

更新日期：2018-04-01 00:00:00

abstract：:Adenosine 5'-phosphosulfate (APS) reductase is a key enzyme involved in the pathways of sulfate reduction and sulfide oxidation in the biological sulfur cycle. In this study, the gene of APS reductase from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the soluble protein was purified by ...

journal_title：Current microbiology

authors： Zheng C,Zhang Y,Liu Y,Wu A,Xia L,Zeng J,Liu J,Qiu G

更新日期：2009-06-01 00:00:00

abstract：:Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the...

journal_title：Current microbiology

authors： Ueno A,Hasanuzzaman M,Yumoto I,Okuyama H

更新日期：2006-03-01 00:00:00

abstract：:Several rhizobial strains possess the ability to modulate leguminous plants ethylene levels by producing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase. While the effect of ACC deaminase has been studied in several rhizobia belonging to the Alphaproteobacteria class, not much is understood about its impa...

journal_title：Current microbiology

authors： Nascimento FX,Tavares MJ,Glick BR,Rossi MJ

更新日期：2018-08-01 00:00:00

abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...

journal_title：Current microbiology

authors： Kim HE,Lee IS,Kim JH,Hahn KW,Park UJ,Han HS,Park KR

更新日期：2003-04-01 00:00:00

abstract：:Cyanide has been proved to be degraded by Klebsiella oxytoca. In order to examine the physiological responses of cyanide degradation by this bacterium, two-dimensional (2-DE) electrophoresis approach and MALDI-TOF-MS allow us to identify 106 proteins spots that were significantly altered in the presence of 1 mM cyanid...

journal_title：Current microbiology

authors： Tang P,Hseu YC,Chou HH,Huang KY,Chen SC

更新日期：2010-03-01 00:00:00

abstract：:The invertase of Lactobacillus reuteri CRL 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kDa. The enzyme was stable below 45 degrees C over a wide pH range (4.5-7.0) with maximum activity at pH 6.0 and 37 degrees C. The invertase activity was significantly inhibited by bivalent meta...

journal_title：Current microbiology

authors： Cuezzo de Ginés S,Maldonado MC,Font de Valdez G

更新日期：2000-03-01 00:00:00

abstract：:A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...

journal_title：Current microbiology

authors： Porteous LA,Armstrong JL,Seidler RJ,Watrud LS

更新日期：1994-11-01 00:00:00

abstract：:A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4-37 °C (optimum: 30 °C) and pH 4-9 (optimum: pH 6), as well as in the presence of 0-6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-sh...

journal_title：Current microbiology

authors： Zhang M,Shen L,Shi J,Chen L,Luo Y,Sun J,Fan D,Hara S,Bao Z

更新日期：2021-01-02 00:00:00

abstract：:Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein wa...

journal_title：Current microbiology

authors： Chen H,Li M,Huang G,Mai W,Chen K,Zhou Y

更新日期：2014-08-01 00:00:00

abstract：:In November 2007, 450 m(3) of treated wastewater leaked into the drinking water distribution system contaminating the drinking water of over 10,000 inhabitants of Nokia, Southern Finland. Nearly 1,000 people visited the health centre because of gastroenteritis during the following 5 weeks. A wide range of enteric path...

journal_title：Current microbiology

authors： Lienemann T,Pitkänen T,Antikainen J,Mölsä E,Miettinen I,Haukka K,Vaara M,Siitonen A

更新日期：2011-04-01 00:00:00

abstract：:By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidi...

journal_title：Current microbiology

authors： Pooler MR,Hartung JS

更新日期：1995-12-01 00:00:00

abstract：:Previously we isolated six actinomycetes strains, 9-4, 10-1, 10-2, 10-3, 10-6, and 21-4, that produce phospholipase D (PLD) with high transphosphatidylation activity. In this study, we identified these strains, and the PLD activities were compared with those of reference strains. 16S rDNA sequences and DNA-DNA hybridi...

journal_title：Current microbiology

authors： Nakazawa Y,Suzuki R,Uchino M,Sagane Y,Kudo T,Nagai T,Sato H,Takano K

更新日期：2010-05-01 00:00:00

abstract：:Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cy...

journal_title：Current microbiology

authors： Jia J,Li Z,Cao J,Jiang Y,Liang C,Liu M

更新日期：2013-10-01 00:00:00

abstract：:Vegetative insecticidal protein (Vip3) from Bacillus thuringiensis shows high activity against lepidopteran insects. Cytolytic δ-endotoxin (Cyt) also has high toxicity to dipteran larvae and synergism with other crystal proteins (Cry), but synergism between Cyt and Vip3 proteins has not been tested. We analyzed for sy...

journal_title：Current microbiology

authors： Yu X,Liu T,Sun Z,Guan P,Zhu J,Wang S,Li S,Deng Q,Wang L,Zheng A,Li P

更新日期：2012-04-01 00:00:00

abstract：:The nucleotide sequences of 16S rDNAs (coding for the small subunit ribosomal RNAs) were used to identify Xylella fastidiosa, a nutritionally fastidious plant pathogenic bacterium. The near-complete 16S rDNAs from nine strains of Xyl. fastidiosa, including seven pathotypes and one strain of Xanthomonas campestris pv. ...

journal_title：Current microbiology

authors： Chen J,Banks D,Jarret RL,Chang CJ,Smith BJ

更新日期：2000-01-01 00:00:00

abstract：:Amino acid residues in the active site of quinoline 2-oxidoreductase (Qor) that are deemed important for substrate binding and turnover were replaced by site-directed mutagenesis. The apparent k(cat) values for quinoline were reduced 2.4-, 38-, 40-, and 199-fold in the protein variants QorA259G, QorW331G, QorV373A, an...

journal_title：Current microbiology

authors： Purvanov V,Fetzner S

更新日期：2005-04-01 00:00:00

abstract：:Thirty-one Listeria strains were tested for sensitivity to four class IIa bacteriocins, namely, enterocin A, mesentericin Y105, divercin V41, and pediocin AcH, and to nisin A. Class IIa bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, wherea...

journal_title：Current microbiology

authors： Ennahar S,Deschamps N,Richard J

更新日期：2000-07-01 00:00:00

abstract：:With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second clone is 69.8-78.7% identical to that of Cry1B group. The nomenclature assignmen...

journal_title：Current microbiology

authors： Kuo WS,Lin JH,Tzeng CC,Kao SS,Chak KF

更新日期：2000-04-01 00:00:00

abstract：:In this study, fatty-acid content and patterns were analyzed in order to distinguish Nostoc flagelliforme, an edible terrestrial cyanobacterium, from other Nostoc species and representatives typical of its close neighbors (genera Anabaena, Microcystis, and Synechococcus). According to the Kenyon-Murata classification ...

journal_title：Current microbiology

authors： Liu XJ,Chen F,Jiang Y

更新日期：2003-12-01 00:00:00

abstract：:A single strain, designated BF49(T), was isolated from a biofilm of a tufa deposit from the Westerhöfer rivulet, Lower Saxony, Germany. The G+C content of the genomic DNA of strain BF49(T) was 69 mol% and the predominant ubiquinone was Q-8. Major fatty acids were C(16:1)omega7c/15 iso 2OH and C(16:0). Comparative 16S ...

journal_title：Current microbiology

authors： Stackebrandt E,Frühling A,Cousin S,Brambilla E,Lünsdorf H,Verbarg S

更新日期：2008-04-01 00:00:00

abstract：:Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pl...

journal_title：Current microbiology

authors： Rai AK,Tiwari SP

更新日期：1999-11-01 00:00:00

abstract：:The organization of the clusters of genes encoding proteins of the botulinum neurotoxin (BoNT) progenitor complex was elucidated in a strain of Clostridium botulinum producing type B and F neurotoxins. With PCR and sequencing strategies, the type B BoNT-gene cluster was found to be composed of genes encoding BoNT/B, n...

journal_title：Current microbiology

authors： Santos-Buelga JA,Collins MD,East AK

更新日期：1998-11-01 00:00:00

abstract：:Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main fl...

journal_title：Current microbiology

authors： Castañón-Rodríguez JF,Pérez-Rodríguez N,de Souza Oliveira RP,Aguilar-Uscanga MG,Domínguez JM

更新日期：2016-10-01 00:00:00

abstract：:Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many species, including pigs, horses and humans. Biofilm formation is essential for pathogenesis, and the ability to resist antibiotic treatment results in difficult-to-treat and persistent infections. However, the ability o...

journal_title：Current microbiology

authors： Yi L,Wang Y,Ma Z,Zhang H,Li Y,Zheng JX,Yang YC,Fan HJ,Lu CP

更新日期：2014-09-01 00:00:00

abstract：:Diseases triggered by microorganisms can be controlled by vaccines, which need neutralizing antigens. Hence, it is very crucial to identify extremely efficient immunogens for immune prevention. Botulism, a fatal neuroparalytic disease, is caused by botulinum neurotoxins produced by the anaerobic, Gram-positive spore-f...

journal_title：Current microbiology

authors： Sharma A,Ponmariappan S,Sarita R,Alam SI,Kamboj DV,Shukla S

更新日期：2018-05-01 00:00:00

abstract：:Bifidobacteria are one of the major components in human microbiota that are suggested to function in maintaining human health. The colonization and cell number of Bifidobacterium species in human intestine vary with ageing. However, sequential changes of Bifidobacterium species ranging from newborns to centenarians re...

journal_title：Current microbiology

authors： Kato K,Odamaki T,Mitsuyama E,Sugahara H,Xiao JZ,Osawa R

更新日期：2017-08-01 00:00:00