Abstract：
:The search for a vaccine capable of conferring heterologous protection, through the identification of conserved and cross-protective antigens, remains an ongoing priority in leptospirosis research. In the present study, an in silico analysis was used to identify potentially protective lipoproteins from Leptospira interrogans serovar Copenhageni. Eight putative lipoproteins were selected (LIC10009, LIC10054, LIC10091, LIC11058, LIC11567, LIC13059, LIC13305, and LIC20172), cloned and expressed in Escherichia coli and purified by affinity chromatography. The recombinant proteins were used to inoculate mice and the subsequent humoral immune response was evaluated by ELISA. Seven of the potential lipoproteins induced a significant IgG response. Furthermore, all of the recombinant proteins were recognized by antibodies present in the sera of severe leptospirosis patients. These putative lipoproteins exhibited potential for further evaluation as prospective vaccine candidates.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Hartwig DD,Seixas FK,Cerqueira GM,McBride AJ,Dellagostin OAdoi
10.1007/s00284-010-9865-1subject
Has Abstractpub_date
2011-04-01 00:00:00pages
1337-41issue
4eissn
0343-8651issn
1432-0991journal_volume
62pub_type
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