Analysis of the Endophytic Bacteria Community Structure and Function of Panax notoginseng Based on High-Throughput Sequencing.

Abstract:

:Panax notoginseng has long been used as a Chinese herb with high medicinal value. The endophytic bacteria in this medicinal plant have multiple biological functions. High-throughput sequencing is a rapidly evolving technique that helps profile the endophytic bacterial community structure of medicinal plants. However, few studies on the endophytic bacteria in P. notoginseng, particularly in dry P. notoginseng roots as a raw medicinal material, have been conducted. In this study, fresh P. notoginseng and dry P. notoginseng were analysed using high-throughput sequencing on an Illumina MiSeq platform to explore the diversity and functions of the endophytic bacteria in different parts of P. notoginseng. The results showed that a total of 201 operational taxonomic units were obtained from fresh P. notoginseng and dry P. notoginseng. The dominant phyla in the fresh and dry P. notoginseng were Proteobacteria (85.9%) and Firmicutes (99.9%), respectively, whereas the dominant genera in these samples were Enterobacter (84.4%) and Bacillus (99.6%), respectively. Fresh P. notoginseng exhibited a higher degree of endophytic bacterial diversity than dry P. notoginseng, but functional prediction of metabolism indicated that the relative abundance of the metabolic function of terpenoids and polyketides synthesis in the dry sample was higher than that in the fresh sample. Our study indicates significant differences in the diversity and metabolic function of the endophytic bacteria between fresh and dry P. notoginseng, providing useful information for the exploitation and utilization of endophytic bacteria resources from P. notoginseng.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Zhang C,Ma X,Zhu R,Liu Z,Gu M,Zhang J,Li Y,Xu Y,Zhu D

doi

10.1007/s00284-020-02068-2

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

2745-2750

issue

10

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-020-02068-2

journal_volume

77

pub_type

杂志文章
  • pH and Not Cell Morphology Modulate pLIP2 Induction in the Dimorphic Yeast Yarrowia lipolytica.

    abstract::The dimorphic yeast Yarrowia lipolytica has become an emerging cell factory for recombinant proteins production. Expression vectors involving LIP2 promoter (pLIP2) have been developed and used successfully. However, the relationship between dimorphic transition (i.e., cell morphology) and pLIP2 regulation is still unc...

    journal_title:Current microbiology

    pub_type: 信件

    doi:10.1007/s00284-017-1207-0

    authors: Sassi H,Delvigne F,Kallel H,Fickers P

    更新日期:2017-03-01 00:00:00

  • Borosilicate Glass Fiber-Optic Biosensor for the Detection of Escherichia coli.

    abstract::Polyclonal antibodies against Escherichia coli and fluorescent, secondary, antibodies were immobilized on borosilicate glass fibers pre-treated with 3-glycidyloxypropyl trimethoxysilane (GPS). Light with an average wavelength of 627 nm, emitted by a diode placed at one end of the glass fiber, was detected by an ultras...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1359-y

    authors: Maas MB,Maybery GHC,Perold WJ,Neveling DP,Dicks LMT

    更新日期:2018-02-01 00:00:00

  • Metabolomics Study of Subsurface Wastewater Infiltration System Under Fluctuation of Organic Load.

    abstract::Subsurface Wastewater Infiltration System (SWIS) is a sewage ecological treatment technology with low investment, energy consumption, and operating cost. SWIS soil contains a large variety of microorganisms. The metabolic process and production of microorganisms are an important basis for qualitatively describing the ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01830-5

    authors: Yang L,Li Y,Su F,Li H

    更新日期:2020-02-01 00:00:00

  • Regulation of alternative oxidase at the transcription stage in Aspergillus niger under the conditions of citric acid production.

    abstract::The citric acid-producing fungus Aspergillus niger WU-2223L possesses a cyanide-insensitive respiratory pathway catalyzed by alternative oxidase. The regulation of the alternative oxidase under the conditions of citric acid production was determined from the transcription level of the alternative oxidase gene (aox1). ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9369-z

    authors: Hattori T,Kino K,Kirimura K

    更新日期:2009-04-01 00:00:00

  • Whole-Genome Analysis of an Extensively Drug-Resistance Empedobacter falsenii Strain Reveals Distinct Features and the Presence of a Novel Metallo-ß-Lactamase (EBR-2).

    abstract::The spread of antibiotic resistance is rapidly threatening the effectiveness of antibiotics in the clinical setting. Many infections are being caused by known and unknown pathogenic bacteria that are resistant to many or all antibiotics currently available. Empedobacter falsenii is a nosocomial pathogen that can cause...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1498-9

    authors: Collins C,Almuzara M,Saigo M,Montaña S,Chiem K,Traglia G,Mussi MA,Tolmasky M,Iriarte A,Vay C,Ramirez MS

    更新日期:2018-08-01 00:00:00

  • Fungal communities in decaying sapwood and heartwood of a conifer Keteleeria evelyniana.

    abstract::Fungal communities in decaying sapwood and heartwood of K. evelyniana were demonstrated through construction of four 18 S rRNA gene libraries. The 210 sequenced clones were clustered into 11 subgroups, belonging to Basidiomycota (71.9%) and to Ascomycota (22.4%) and unclassified (1 subgroup; 5.7%). The heartwood displ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-007-9092-6

    authors: Zhang HB,Yang MX,Tu R,Gao L,Zhao ZW

    更新日期:2008-04-01 00:00:00

  • Luteimonas weifangensis sp. nov., Isolated from Bensulfuron-Methyl Contaminated Watermelon Soil.

    abstract::A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02210-0

    authors: Zhang H,Zhao HZ,Liu J,Qin W,Huang X

    更新日期:2020-11-01 00:00:00

  • Identification of household bacterial community and analysis of species shared with human microbiome.

    abstract::Microbial populations in indoor environments, where we live and eat, are important for public health. Various bacterial species reside in the kitchen, and refrigerators, the major means of food storage within kitchens, can be a direct source of food borne illness. Therefore, the monitoring of microbiota in the refrige...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0401-y

    authors: Jeon YS,Chun J,Kim BS

    更新日期:2013-11-01 00:00:00

  • Changes in bacterial communities accompanied by aggregation in a fed-batch composting reactor.

    abstract::The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9107-y

    authors: Watanabe K,Nagao N,Toda T,Kurosawa N

    更新日期:2008-05-01 00:00:00

  • Description of a novel actinobacterium Microbacterium assamensis sp. nov., isolated from water sample collected from the river Brahmaputra, Assam, India.

    abstract::A Gram-positive, yellow pigmented actinobacterium, strain S2-48(T) was isolated from water sample collected from the river Brahmaputra, Assam, India and subjected to a polyphasic taxonomic study. Most of the physiological and biochemical properties, major fatty acids (C(15:0) Anteiso, iso C(16:0) and C(17:0) Anteiso),...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9822-z

    authors: Kaur I,Dhanjal S,Korpole S,Bora TC,Mayilraj S

    更新日期:2011-03-01 00:00:00

  • Roles of RPS41 in Biofilm Formation, Virulence, and Hydrogen Peroxide Sensitivity in Candida albicans.

    abstract::In eukaryotes, loss of cytoplasmic ribosomal proteins (RPs) results in a reduced growth rate and other phenotypic defects. The ability to transition from a unicellular budding yeast to a filamentous form is very important for biofilm formation and virulence in Candida albicans. Our recent study found that loss of the ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-016-1019-7

    authors: Lu H,Xiong J,Shang Q,Jiang Y,Cao Y

    更新日期:2016-06-01 00:00:00

  • Identification and Characterization of Pediococcus Species from Piper betle (Betel) Leaves.

    abstract::Betel vine is an edible creeper used in folk medicine to aid digestion since time immemorial. It is an ideal candidate deemed for the bioprospection of endophytic microorganisms with valuable attributes. This study aimed at the characterization of potential bacteria from fermented betel leaves. We report the presence ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02270-2

    authors: Keerthana C,Narayanan RB

    更新日期:2021-01-01 00:00:00

  • Evaluating Protocols for Porcine Faecal Microbiome Recollection, Storage and DNA Extraction: from the Farm to the Lab.

    abstract::There is a growing interest in understanding the role of the gut microbiome on productive and meat quality-related traits in livestock species in order to develop new useful tools for improving pig production systems and industry. Faecal samples are analysed as a proxy of gut microbiota and here the selection of suita...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1429-1

    authors: Muiños-Bühl A,González-Recio O,Muñoz M,Óvilo C,García-Casco J,Fernández AI

    更新日期:2018-06-01 00:00:00

  • Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria.

    abstract::In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biof...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-016-1080-2

    authors: Olofsson TC,Butler É,Lindholm C,Nilson B,Michanek P,Vásquez A

    更新日期:2016-10-01 00:00:00

  • Insecticidal activity of the protein encoded by the cryV gene of Bacillus thuringiensis kurstaki INA-02.

    abstract::A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase ch...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900035

    authors: Sasaki J,Asano S,Iizuka T,Bando H,Lay BW,Hastowo S,Powell GK,Yamamoto T

    更新日期:1996-04-01 00:00:00

  • Biofilm formation by Salmonella enterica serovar 1,4,[5],12:i:- Portuguese isolates: a phenotypic, genotypic, and socio-geographic analysis.

    abstract::Biofilm-forming ability is well established as an important virulence factor. However, there are no studies available regarding biofilm formation of Salmonella Typhimurium 1,4,[5],12:i:-, the new pandemic serovar in Europe. To address this problem, biofilm expression by Salmonella 1,4,[5],12:i:- was evaluated using 13...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0523-x

    authors: Seixas R,Machado J,Bernardo F,Vilela C,Oliveira M

    更新日期:2014-05-01 00:00:00

  • Possible occurrence of a Crabtree effect in the production of lactic and butyric acids by a floc-forming bacterial consortium.

    abstract::Lactic and butyric acid production by bacterial flocs in a continuous culture obeyed different physiological constraints. The butyric acid rate of production was constant and independent of the growth rate [0.012 +/- 0.001 gBUT/(L.h)], whereas lactic fermentation occurred only beyond a critical growth rate (0.25 +/- 0...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-003-4169-3

    authors: Thierie J,Penninckx MJ

    更新日期:2004-03-01 00:00:00

  • Antiviral effect of diammonium glycyrrhizinate on cell infection by porcine parvovirus.

    abstract::Porcine parvovirus (PPV) can cause reproductive failure in swine, resulting in economic losses to the industry. Antiviral effects of diammonium glycyrrhizinate (DG) have been reported on several animal viruses; however, to date it has yet to be tested on PPV. In this study, the antiviral activity of DG on swine testis...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0540-9

    authors: Li P,Zou H,Ren Y,Zarlenga DS,Ren X

    更新日期:2014-07-01 00:00:00

  • First Evidence of Intracellular Bacteria Cardinium in Thermophilic Mite Microzetorchestes emeryi (Acari: Oribatida): Molecular Screening of Bacterial Endosymbiont Species.

    abstract::We undertook the issue of the distribution of intracellular bacteria among Oribatida (Acari). Six genera of bacteria were detected by PCR and Sanger DNA sequencing: Wolbachia, Cardinium, Rickettsia, Spiroplasma, Arsenophonus, and Hamiltonella. Our research, for the first time, revealed the presence of Cardinium in Mic...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01717-5

    authors: Konecka E,Olszanowski Z

    更新日期:2019-09-01 00:00:00

  • Correlation between in vitro secretion of virulence-associated proteins of Campylobacter jejuni and colonization of chickens.

    abstract::The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0295-z

    authors: Biswas D,Fernando UM,Reiman CD,Willson PJ,Townsend HG,Potter AA,Allan BJ

    更新日期:2007-03-01 00:00:00

  • Differences in composition and mucosal adhesion of bifidobacteria isolated from healthy adults and healthy seniors.

    abstract::Fifty-one Bifidobacterium strains were isolated from the feces of healthy adults (30-40 years old) and seniors (older than 70 years of age). B. adolescentis, B. breve, B. infantis, and B. longum were isolated from the healthy adults and B. adolescentis and B. longum from elderly subjects. The tested bacteria bound, in...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002840010315

    authors: He F,Ouwehand AC,Isolauri E,Hosoda M,Benno Y,Salminen S

    更新日期:2001-11-01 00:00:00

  • Fission Yeast srm1 is Involved in Stress Response and Cell Cycle.

    abstract::Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this st...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1241-y

    authors: Gevrekci AÖ

    更新日期:2017-06-01 00:00:00

  • Isolation and characterization of new cyclohexylacetic acid-degrading bacteria.

    abstract::Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9158-0

    authors: Iwaki H,Nakai E,Nakamura S,Hasegawa Y

    更新日期:2008-08-01 00:00:00

  • Tinea Gladiatorum and Dermatophyte Contamination Among Wrestlers and in Wrestling Halls: A Systematic Review and Meta-analysis.

    abstract::To present an overview of the existing epidemiological evidence regarding the occurrence of tinea gladiatorum in wrestlers and dermatophyte contamination in wrestling halls, five electronic databases including "PubMed," "Scopus," "Google Scholar," "Science Direct," and "Web of Science" were searched from inception to ...

    journal_title:Current microbiology

    pub_type: 杂志文章,meta分析

    doi:10.1007/s00284-019-01816-3

    authors: Kermani F,Moosazadeh M,Hosseini SA,Bandalizadeh Z,Barzegari S,Shokohi T

    更新日期:2020-04-01 00:00:00

  • Bacterial growth on coral mucus.

    abstract::Coral mucus-degrading bacteria were isolated by an enrichment culture procedure. The isolates were able to grow as pure cultures on 10% sterilized mucus in seawater, yielding 10(8) CFU/ml. The isolates, mostly Vibrio strains, were classified by classical and molecular methods. When carbon, nitrogen, and phosphorus com...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9100-5

    authors: Sharon G,Rosenberg E

    更新日期:2008-05-01 00:00:00

  • Analysis of cry gene profiles in Bacillus thuringiensis strains isolated during epizootics in Cydia pomonella L.

    abstract::The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crys...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-007-0085-2

    authors: Konecka E,Kaznowski A,Ziemnicka J,Ziemnicki K,Paetz H

    更新日期:2007-09-01 00:00:00

  • Secondary endosymbionts of psyllids have been acquired multiple times.

    abstract::Previous studies have established that psyllids (Hemiptera, Psylloidea) contain primary endosymbionts, designated as Carsonella ruddii, which cospeciate with the psyllid host. This association appears to be the consequence of a single infection of a psyllid ancestor with a bacterium. Some psyllids may have additional ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002840010138

    authors: Thao ML,Clark MA,Baumann L,Brennan EB,Moran NA,Baumann P

    更新日期:2000-10-01 00:00:00

  • Antimicrobial susceptibility profiles of Salmonella enterica serotypes recovered from pens of commercial feedlot cattle using different types of composite samples.

    abstract::Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9347-x

    authors: Alam MJ,Renter D,Taylor E,Mina D,Moxley R,Smith D

    更新日期:2009-04-01 00:00:00

  • Microbulbifer arenaceous sp. nov., a new endolithic bacterium isolated from the inside of red sandstone.

    abstract::An endolithic bacterium, strain RSBr-1, was isolated from the inside of a piece of red sandstone from coastal areas of Scotland. RSBr-1 was gram negative, oxidase and catalase positive, and cells were non-motile rods. Sodium was required for growth. The optimum sodium chloride concentration and pH for growth were 4% a...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-003-4006-8

    authors: Tanaka T,Yan L,Burgess JG

    更新日期:2003-11-01 00:00:00

  • Identification of a novel virulence-related gene in Streptococcus suis type 2 strains.

    abstract::The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9643-0

    authors: Zhang H,Fan H,Lu C

    更新日期:2010-12-01 00:00:00