Abstract：
:Ansamitocins are extraordinarily potent antitumor agents. Ansamitocin P-3 (AP-3), which is produced by Actinosynnema pretiosum, has been developed as a cytotoxic drug for breast cancer. Despite its importance, AP-3 is of limited applicability because of the low production yield. A. pretiosum strain X47 was developed from A. pretiosum ATCC 31565 by mutation breeding and shows a relatively high AP-3 yield. Here, we analyzed the A. pretiosum X47 genome, which is ~8.13 Mb in length with 6693 coding sequences, 58 tRNA genes, and 15 rRNA genes. The DNA sequence of the ansamitocin biosynthetic gene cluster is highly similar to that of the corresponding cluster in A. pretiosum ATCC 31565, with 99.9% identity. However, RT-qPCR analysis showed that the expression levels of ansamitocin biosynthetic genes were significantly increased in X47 compared with the levels in the wild-type strain, consistent with the higher yield of AP-3 in X47. The annotated complete genome sequence of this strain will facilitate understanding the molecular mechanisms of ansamitocin biosynthesis and regulation in A. pretiosum and help further genetic engineering studies to enhance the production of AP-3.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Zhong C,Zong G,Qian S,Liu M,Fu J,Zhang P,Li J,Cao Gdoi
10.1007/s00284-018-1521-1subject
Has Abstractpub_date
2019-08-01 00:00:00pages
954-958issue
8eissn
0343-8651issn
1432-0991pii
10.1007/s00284-018-1521-1journal_volume
76pub_type
信件abstract：:A broad-host-range vector that expresses a unique artificial RNA in Pseudomonas putida has been developed. This vector was derived from the plasmid pBBR1MCS and incorporates regulatory regions from the Escherichia coli ribosomal operon, rrnB. These include the promoters P1 and P2, and the terminators T1 and T2. The ge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910017
更新日期：2000-02-01 00:00:00
abstract：:All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02150-9
更新日期：2020-11-01 00:00:00
abstract：:By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron-sulfur clusters. But so far, there were no articles to confirm the existence of iron-sulfur cluster in RhdA. In this study, R...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9476-x
更新日期：2009-11-01 00:00:00
abstract：:The diversities of soil bacterial communities in the Phragmites australis, Phalaris arundinacea, and non-plantation constructed wetlands were compared and analyzed by through high-throughput Illumina sequencing. At the phylum level, the ten dominant bacterial phyla in the three types of constructed wetlands were the s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01634-7
更新日期：2019-03-01 00:00:00
abstract：:Triterpenoid saponin derivatives oleanolic acid (OA) and ursolic acid (UA), but not betulinic acid (BA), were previously found to have strong antimicrobial activity against Streptococcus mutans. OA and UA inhibited the transcription of genes related to peptidoglycan biosynthesis, thereby preventing bacterial growth. H...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1344-5
更新日期：2018-01-01 00:00:00
abstract：:A Gram-negative, yellow pigmented bacterium designated strain CA5(T) that reduced selenite to elemental red selenium was isolated from soil. 16S rRNA gene sequence alignment identified the isolate as a novel Pseudomonas species with P. argentinensis, P. flavescens, and P. straminea as its closest relatives. Sequence a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9745-8
更新日期：2011-02-01 00:00:00
abstract：:We constructed recombinant phage particles displaying the Bacillus thuringiensis Cry1Ba4 active toxin using the pfUSE5 and pComb3X phagemid vectors. The recombinant phage particles were screened and evaluated for displayed biologically active Cry1Ba4 toxin against the target insect larvae. Concurrent expression of Cry...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0164-9
更新日期：2006-11-01 00:00:00
abstract：:The first cloning and characterization of the gene katA, encoding the major catalase (KatA), from Xanthomonas is reported. A reverse genetic approach using a synthesized katA-specific DNA probe to screen a X. campestris pv. phaseoli genomic library was employed. A positively hybridizing clone designated pKat29 that co...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3812-8
更新日期：2003-02-01 00:00:00
abstract：:The adhesive property to the intestinal mucin of Bifidobacterium lactis LKM512, B. longum, B. breve, B. bifidum, B. adolescentis, B. infantis, Bacteroides vulgatus, Bacteroides distasonis, Eubacterium aerofaciens, Clostridium perfringens, Escherichia coli, and Lactobacillus acidophilus were examined. Adhesive rate of ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0087-4
更新日期：2002-03-01 00:00:00
abstract：:To isolate a naturally occurring novel Bacillus thuringiensis strain, we investigated the distribution, toxicity, morphology, H serotype, and gene type of B. thuringiensis from residue samples of granary in Korea. A total of 163 B. thuringiensis isolates out of 411 samples producing spore and crystal were obtained. In...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900336
更新日期：1998-07-01 00:00:00
abstract：:One of the main requirements for probiotics is their ability to survive during passage through gastrointestinal tract and to maintain their activity at different adverse conditions. The aim of the study was to look for the strains of lactobacilli and streptococci with high adhesive properties even affected by inhibito...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1017-9
更新日期：2016-06-01 00:00:00
abstract：:Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006756
更新日期：1998-03-01 00:00:00
abstract：:The Gram-stain-negative, rod-shaped, aerobic strain, designated YC1039T, was isolated from a seamount northern Mariana Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YC1039T was related to the genus Mesoflavibacter and had highest 16S rRNA gene sequence...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1500-6
更新日期：2018-09-01 00:00:00
abstract：:Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900421
更新日期：1999-08-01 00:00:00
abstract：:Culture-based analysis was employed in parallel with PCR amplification of 16S rDNA, coupled with denaturing gradient gel electrophoresis (DGGE), to profile bacterial species associated with different developmental stages of the pine false webworm (PFW), Acantholyda erythrocephala, a sawfly pest responsible for inciden...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9243-4
更新日期：2008-12-01 00:00:00
abstract：:Recently, we showed that the southern green stink bug (SGSB), Nezara viridula (L.), can transmit Pantoea agglomerans (Ewing and Fife), an opportunistic bacterium, into green cotton bolls resulting in plant disease. Here, we hypothesized that our established model could be used to determine if the SGSB was a general, n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9452-5
更新日期：2009-10-01 00:00:00
abstract：:Potassium tellurite (K2TeO3) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K2TeO3 resistance in STEC is attributable to the ter gene cluster with terD an indicator of the c...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1444-x
更新日期：2018-06-01 00:00:00
abstract：:Adenosine 5'-phosphosulfate (APS) reductase is a key enzyme involved in the pathways of sulfate reduction and sulfide oxidation in the biological sulfur cycle. In this study, the gene of APS reductase from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the soluble protein was purified by ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9375-1
更新日期：2009-06-01 00:00:00
abstract：:This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9038-z
更新日期：2008-01-01 00:00:00
abstract：:The cbbF genes of the facultative chemoautotroph Alcaligenes eutrophus H16 are part of two highly homologous cbb operons. Both the chromosomal and the megaplasmid pHG1-borne copy of cbbF were cloned and sequenced. Subsequent analyses including comparison with known sequences from other organisms and heterologous expre...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00294635
更新日期：1995-07-01 00:00:00
abstract：:A new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3978-0
更新日期：2003-08-01 00:00:00
abstract：:Rhodococcus sp. Eu-32 has shown an extended novel dibenzothiophene desulfurization sulfur-specific 4S pathway and could remove significant amounts of organic sulfur from coal. Here, we present the draft genome sequence of Eu-32 with a genome size of approximately 5.61 Mb, containing 5065 protein coding sequences with ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01737-1
更新日期：2019-10-01 00:00:00
abstract：:The pathogenic capability of the tangerine pathotype of Alternaria alternata relies on the production of host-selective ACT toxin. Inoculation of A. alternata in leaves of the citrus quickly induced rapid lipid peroxidation, accumulation of hydrogen peroxide (H(2)O(2)), and cell death, indicative of host defensive res...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9795-y
更新日期：2011-03-01 00:00:00
abstract：:Amino acid residues in the active site of quinoline 2-oxidoreductase (Qor) that are deemed important for substrate binding and turnover were replaced by site-directed mutagenesis. The apparent k(cat) values for quinoline were reduced 2.4-, 38-, 40-, and 199-fold in the protein variants QorA259G, QorW331G, QorV373A, an...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4452-y
更新日期：2005-04-01 00:00:00
abstract：:Bacillus sp. TYF-LIM-B05, which is isolated from spoilage vinegar, is resistant to high temperature, high concentrated alcohol, acid, and salt, and can produce ethanol from mono-, di-, polysaccharide, and complex biomass as the sole carbon source. Thus, this strain is a potential candidate for consolidated bioprocessi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01833-2
更新日期：2020-03-01 00:00:00
abstract：:A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9T, was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9T belonge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1246-6
更新日期：2017-07-01 00:00:00
abstract：:A Gram-stain-negative, rod-shaped bacterial strain JS15-10A1T was isolated from oil production water. Its optimum growth was observed at 37 °C, pH 7.0, and 3% (w/v) NaCl. The 16S rRNA gene sequence of strain JS15-10A1T showed the highest similarities with Pseudomonas parafulva CB-1T (97.6%) and P. fulva IAM 1529T (97....
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-019-01798-2
更新日期：2020-04-01 00:00:00
abstract：:The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected str...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1428-2
更新日期：2018-05-01 00:00:00
abstract：:An isolate of Lactococcus lactis from fermented milk was found to produce a bacteriocin peptide. The isolate could grow in a medium with an initial pH of 11.0, in which it produced the bacteriocin extracellularly at the highest level. The level of the bacteriocin in the medium increased in parallel to the bacterial gr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4545-2
更新日期：2005-09-01 00:00:00
abstract：:The outer membrane protein of Photobacterium damsela (OMP-PD) and the gene encoding for this porin protein were isolated and characterized. The deduced amino acid sequence of the OMP-PD monomer has 338 amino acids and a calculated molecular weight of 36,951 Da. This sequence includes a 22-amino acid signal peptide at ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4111-8
更新日期：2004-03-01 00:00:00
