Structures of human DNA polymerases ν and θ expose their end game.

abstract：:The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein-RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to s...

journal_title：Nature structural & molecular biology

authors： Chao JA,Patskovsky Y,Almo SC,Singer RH

更新日期：2008-01-01 00:00:00

abstract：:The Ca(2+) sensor synaptotagmin-1 is thought to trigger membrane fusion by binding to acidic membrane lipids and SNARE proteins. Previous work has shown that binding is mediated by electrostatic interactions that are sensitive to the ionic environment. However, the influence of divalent or polyvalent ions, at physiolo...

journal_title：Nature structural & molecular biology

authors： Park Y,Seo JB,Fraind A,Pérez-Lara A,Yavuz H,Han K,Jung SR,Kattan I,Walla PJ,Choi M,Cafiso DS,Koh DS,Jahn R

更新日期：2015-10-01 00:00:00

abstract：:Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions. Here we show that fusion driven by neuronal SNAREs is inhibited by the regulatory protein complexin. Furthermore, inner-leaflet mixing is strongly impaired relative to total lipid mixing, indicating that inhibition by c...

journal_title：Nature structural & molecular biology

authors： Schaub JR,Lu X,Doneske B,Shin YK,McNew JA

更新日期：2006-08-01 00:00:00

abstract：:Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells that is triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation followed by degradation of nascent peptides. However, endogenous RQC-inducing sequences and the mechanism underlying the...

journal_title：Nature structural & molecular biology

authors： Matsuo Y,Tesina P,Nakajima S,Mizuno M,Endo A,Buschauer R,Cheng J,Shounai O,Ikeuchi K,Saeki Y,Becker T,Beckmann R,Inada T

更新日期：2020-04-01 00:00:00

abstract：:Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryot...

journal_title：Nature structural & molecular biology

authors： Losey HC,Ruthenburg AJ,Verdine GL

更新日期：2006-02-01 00:00:00

abstract：:Aquaporin (AQP) folding in the endoplasmic reticulum is characterized by two distinct pathways of membrane insertion that arise from divergent residues within the second transmembrane segment. We now show that in AQP1 these residues (Asn49 and Lys51) interact with Asp185 at the C terminus of TM5 to form a polar, quate...

journal_title：Nature structural & molecular biology

authors： Buck TM,Wagner J,Grund S,Skach WR

更新日期：2007-08-01 00:00:00

abstract：:Desensitization is a universal feature of ligand-gated ion channels. Using the crystal structure of the GluR2 L483Y mutant channel as a guide, we attempted to build non-desensitizing kainate-subtype glutamate receptors. Success was achieved for GluR5, GluR6 and GluR7 with intermolecular disulfide cross-links but not b...

journal_title：Nature structural & molecular biology

authors： Weston MC,Schuck P,Ghosal A,Rosenmund C,Mayer ML

更新日期：2006-12-01 00:00:00

abstract：:Integral membrane proteins are generally believed to have unique membrane topologies. However, it has been suggested that dual-topology proteins that adopt a mixture of two opposite orientations in the membrane may exist. Here we show that the membrane orientations of five dual-topology candidates identified in Escher...

journal_title：Nature structural & molecular biology

authors： Rapp M,Granseth E,Seppälä S,von Heijne G

更新日期：2006-02-01 00:00:00

abstract：:P element transposase catalyzes the mobility of P element DNA transposons within the Drosophila genome. P element transposase exhibits several unique properties, including the requirement for a guanosine triphosphate cofactor and the generation of long staggered DNA breaks during transposition. To gain insights into t...

journal_title：Nature structural & molecular biology

authors： Ghanim GE,Kellogg EH,Nogales E,Rio DC

更新日期：2019-11-01 00:00:00

abstract：:Induced transcription through CAG repeats in human cells increases repeat contraction approximately 15-fold in both confluent and proliferating cells. Repeats are stabilized against contraction by siRNA knockdown of MSH2, MSH3 or XPA, but not by knockdown of MSH6, XPC or FEN1. These results define a pathway for CAG.CT...

journal_title：Nature structural & molecular biology

authors： Lin Y,Dion V,Wilson JH

更新日期：2006-02-01 00:00:00

abstract：:Tripeptidyl peptidase II (TPP II) is the largest known eukaryotic protease (6 MDa). It is believed to act downstream of the 26S proteasome, cleaving tripeptides from the N termini of longer peptides, and it is implicated in numerous cellular processes. Here we report the structure of Drosophila TPP II determined by a ...

journal_title：Nature structural & molecular biology

authors： Chuang CK,Rockel B,Seyit G,Walian PJ,Schönegge AM,Peters J,Zwart PH,Baumeister W,Jap BK

更新日期：2010-08-01 00:00:00

abstract：:Platelet-activating-factor receptor (PAFR) responds to platelet-activating factor (PAF), a phospholipid mediator of cell-to-cell communication that exhibits diverse physiological effects. PAFR is considered an important drug target for treating asthma, inflammation and cardiovascular diseases. Here we report crystal s...

journal_title：Nature structural & molecular biology

authors： Cao C,Tan Q,Xu C,He L,Yang L,Zhou Y,Zhou Y,Qiao A,Lu M,Yi C,Han GW,Wang X,Li X,Yang H,Rao Z,Jiang H,Zhao Y,Liu J,Stevens RC,Zhao Q,Zhang XC,Wu B

更新日期：2018-06-01 00:00:00

abstract：:Ribosomes catalyze the formation of peptide bonds between aminoacyl esters of transfer RNAs within a catalytic center composed of ribosomal RNA only. Here we show that the reaction of P-site formylmethionine (fMet)-tRNA(fMet) with a modified A-site tRNA substrate, Phelac-tRNA(Phe), in which the nucleophilic amino grou...

journal_title：Nature structural & molecular biology

authors： Bieling P,Beringer M,Adio S,Rodnina MV

更新日期：2006-05-01 00:00:00

abstract：: ...

journal_title：Nature structural & molecular biology

authors： Horowitz S,Salmon L,Koldewey P,Ahlstrom LS,Martin R,Quan S,Afonine PV,van den Bedem H,Wang L,Xu Q,Trievel RC,Brooks CL 3rd,Bardwell JCA

更新日期：2018-11-01 00:00:00

abstract：:Complex forms of cellular motility, including cell division, organelle trafficking or signal amplification in the auditory system, require strong coordination of the myosin motors involved. The most basic mechanism of coordination is via direct mechanical interactions of individual motor heads leading to modification ...

journal_title：Nature structural & molecular biology

authors： Sellers JR,Veigel C

更新日期：2010-05-01 00:00:00

abstract：:The function of human XPA protein, a key subunit of the nucleotide excision repair pathway, has been examined with site-directed substitutions in its putative DNA-binding cleft. After screening for repair activity in a host-cell reactivation assay, we analyzed mutants by comparing their affinities for different substr...

journal_title：Nature structural & molecular biology

authors： Camenisch U,Dip R,Schumacher SB,Schuler B,Naegeli H

更新日期：2006-03-01 00:00:00

abstract：:STING functions as both an adaptor protein signaling cytoplasmic double-stranded DNA and a direct immunosensor of cyclic diguanylate monophosphate (c-di-GMP). The crystal structures of the C-terminal domain of human STING (STING(CTD)) and its complex with c-di-GMP reveal how STING recognizes c-di-GMP. In response to c...

journal_title：Nature structural & molecular biology

authors： Shang G,Zhu D,Li N,Zhang J,Zhu C,Lu D,Liu C,Yu Q,Zhao Y,Xu S,Gu L

更新日期：2012-06-24 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Weill L,Belloc E,Castellazzi CL,Méndez R

更新日期：2019-08-01 00:00:00

abstract：:Polycomb repressive complex 2 (PRC2) trimethylates histone H3 at lysine 27 to mark genes for repression. We measured the dynamics of PRC2 binding on recombinant chromatin and free DNA at the single-molecule level using total internal reflection fluorescence (TIRF) microscopy. PRC2 preferentially binds free DNA with mu...

journal_title：Nature structural & molecular biology

authors： Choi J,Bachmann AL,Tauscher K,Benda C,Fierz B,Müller J

更新日期：2017-12-01 00:00:00

abstract：:Obesity and type II diabetes are closely linked metabolic syndromes that afflict >100 million people worldwide. Although protein tyrosine phosphatase 1B (PTP1B) has emerged as a promising target for the treatment of both syndromes, the discovery of pharmaceutically acceptable inhibitors that bind at the active site re...

journal_title：Nature structural & molecular biology

authors： Wiesmann C,Barr KJ,Kung J,Zhu J,Erlanson DA,Shen W,Fahr BJ,Zhong M,Taylor L,Randal M,McDowell RS,Hansen SK

更新日期：2004-08-01 00:00:00

abstract：:During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation comp...

journal_title：Nature structural & molecular biology

authors： Bernecky C,Plitzko JM,Cramer P

更新日期：2017-10-01 00:00:00

abstract：:The endosomal sorting complex required for transport (ESCRT) machinery, including ESCRT-III, localizes to the midbody and participates in the membrane-abscission step of cytokinesis. The ESCRT-III protein charged multivesicular body protein 1B (CHMP1B) is required for recruitment of the MIT domain-containing protein s...

journal_title：Nature structural & molecular biology

authors： Yang D,Rismanchi N,Renvoisé B,Lippincott-Schwartz J,Blackstone C,Hurley JH

更新日期：2008-12-01 00:00:00

abstract：:Structural maintenance of chromosome (SMC) complexes are central regulators of chromosome architecture that are essential in all domains of life. For decades, the structural biology field has been debating how these conserved protein complexes use their intricate ring-like structures to structurally organize DNA. Here...

journal_title：Nature structural & molecular biology

authors： Eeftens J,Dekker C

更新日期：2017-12-07 00:00:00

abstract：:Mycobacterium tuberculosis uses a proteasome system that is analogous to the eukaryotic ubiquitin-proteasome pathway and is required for pathogenesis. However, the bacterial analog of ubiquitin, prokaryotic ubiquitin-like protein (Pup), is an intrinsically disordered protein that bears little sequence or structural re...

journal_title：Nature structural & molecular biology

authors： Wang T,Darwin KH,Li H

更新日期：2010-11-01 00:00:00

abstract：:Herpesviruses, which cause many incurable diseases, infect cells by fusing viral and cellular membranes. Whereas most other enveloped viruses use a single viral catalyst called a fusogen, herpesviruses, inexplicably, require two conserved fusion-machinery components, gB and the heterodimer gH-gL, plus other nonconserv...

journal_title：Nature structural & molecular biology

authors： Chowdary TK,Cairns TM,Atanasiu D,Cohen GH,Eisenberg RJ,Heldwein EE

更新日期：2010-07-01 00:00:00

abstract：:We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibo...

journal_title：Nature structural & molecular biology

authors： Egelhofer TA,Minoda A,Klugman S,Lee K,Kolasinska-Zwierz P,Alekseyenko AA,Cheung MS,Day DS,Gadel S,Gorchakov AA,Gu T,Kharchenko PV,Kuan S,Latorre I,Linder-Basso D,Luu Y,Ngo Q,Perry M,Rechtsteiner A,Riddle NC,Schwar

更新日期：2011-01-01 00:00:00

abstract：:Noncoding RNAs, mobile elements, and alternative splicing are all critical for the regulation of gene expression. Here we show that a conserved noncoding RNA acquires a new function due to the insertion of a mobile element. We identified a noncoding RNA, termed 5S-OT, which is transcribed from 5S rDNA loci in eukaryot...

journal_title：Nature structural & molecular biology

authors： Hu S,Wang X,Shan G

更新日期：2016-11-01 00:00:00

abstract：:A fundamental question is how enzymes can accelerate chemical reactions. Catalysis is not only defined by actual chemical steps, but also by enzyme structure and dynamics. To investigate the role of protein dynamics in enzymatic turnover, we measured residue-specific protein dynamics in hyperthermophilic and mesophili...

journal_title：Nature structural & molecular biology

authors： Wolf-Watz M,Thai V,Henzler-Wildman K,Hadjipavlou G,Eisenmesser EZ,Kern D

更新日期：2004-10-01 00:00:00

abstract：:Heterochromatin causes epigenetic repression that can be transmitted through multiple cell divisions. However, the mechanisms underlying silencing and stability of heterochromatin are not fully understood. We show that heterochromatin differs from euchromatin in histone turnover and identify histone deacetylase (HDAC)...

journal_title：Nature structural & molecular biology

authors： Aygün O,Mehta S,Grewal SI

更新日期：2013-05-01 00:00:00

abstract：:The N-terminal acetylation of Sir3 is essential for heterochromatin establishment and maintenance in yeast, but its mechanism of action is unknown. The crystal structure of the N-terminally acetylated BAH domain of Saccharomyces cerevisiae Sir3 bound to the nucleosome core particle reveals that the N-terminal acetylat...

journal_title：Nature structural & molecular biology

authors： Arnaudo N,Fernández IS,McLaughlin SH,Peak-Chew SY,Rhodes D,Martino F

更新日期：2013-09-01 00:00:00