Abstract:
:P element transposase catalyzes the mobility of P element DNA transposons within the Drosophila genome. P element transposase exhibits several unique properties, including the requirement for a guanosine triphosphate cofactor and the generation of long staggered DNA breaks during transposition. To gain insights into these features, we determined the atomic structure of the Drosophila P element transposase strand transfer complex using cryo-EM. The structure of this post-transposition nucleoprotein complex reveals that the terminal single-stranded transposon DNA adopts unusual A-form and distorted B-form helical geometries that are stabilized by extensive protein-DNA interactions. Additionally, we infer that the bound guanosine triphosphate cofactor interacts with the terminal base of the transposon DNA, apparently to position the P element DNA for catalysis. Our structure provides the first view of the P element transposase superfamily, offers new insights into P element transposition and implies a transposition pathway fundamentally distinct from other cut-and-paste DNA transposases.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Ghanim GE,Kellogg EH,Nogales E,Rio DCdoi
10.1038/s41594-019-0319-6subject
Has Abstractpub_date
2019-11-01 00:00:00pages
1013-1022issue
11eissn
1545-9993issn
1545-9985pii
10.1038/s41594-019-0319-6journal_volume
26pub_type
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