Abstract:
:We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Egelhofer TA,Minoda A,Klugman S,Lee K,Kolasinska-Zwierz P,Alekseyenko AA,Cheung MS,Day DS,Gadel S,Gorchakov AA,Gu T,Kharchenko PV,Kuan S,Latorre I,Linder-Basso D,Luu Y,Ngo Q,Perry M,Rechtsteiner A,Riddle NC,Schwardoi
10.1038/nsmb.1972subject
Has Abstractpub_date
2011-01-01 00:00:00pages
91-3issue
1eissn
1545-9993issn
1545-9985pii
nsmb.1972journal_volume
18pub_type
杂志文章abstract:: ...
journal_title:Nature structural & molecular biology
pub_type: 评论,信件
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章,评审
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abstract::TRIMCyps are primate antiretroviral proteins that potently inhibit HIV replication. Here we describe how rhesus macaque TRIMCyp (RhTC) has evolved to target and restrict HIV-2. We show that the ancestral cyclophilin A (CypA) domain of RhTC targets HIV-2 capsid with weak affinity, which is strongly increased in RhTC by...
journal_title:Nature structural & molecular biology
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2011-06-05 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2016-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章,评审
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pub_type: 已发布勘误
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更新日期:2018-08-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2017-05-04 00:00:00
abstract:: ...
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pub_type: 新闻
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更新日期:2018-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2011-06-19 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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