An assessment of histone-modification antibody quality.

Abstract:

:We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).

journal_name

Nat Struct Mol Biol

authors

Egelhofer TA,Minoda A,Klugman S,Lee K,Kolasinska-Zwierz P,Alekseyenko AA,Cheung MS,Day DS,Gadel S,Gorchakov AA,Gu T,Kharchenko PV,Kuan S,Latorre I,Linder-Basso D,Luu Y,Ngo Q,Perry M,Rechtsteiner A,Riddle NC,Schwar

doi

10.1038/nsmb.1972

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

91-3

issue

1

eissn

1545-9993

issn

1545-9985

pii

nsmb.1972

journal_volume

18

pub_type

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